Preparation and Characterization of β-Tricalcium Phosphate Powders with High Solubility for Chelate-Setting Calcium-Phosphate Cements

2017 ◽  
Vol 758 ◽  
pp. 194-198
Author(s):  
Kohei Nagata ◽  
Toshiisa Konishi ◽  
Michiyo Honda ◽  
Mamoru Aizawa
Keyword(s):  
Tricalcium Phosphate ◽  
Single Phase ◽  
Inositol Phosphate ◽  
Heating Temperature ◽  
Japanese Industrial Standard ◽  
Bone Filler ◽  
Setting Mechanism ◽  
Median Particle

We have previously developed a novel chelate-setting β-tricalcium phosphate (β-TCP) cement with non-fragmentation property in vivo. This novel cement has been set on the basis of chelate-setting mechanism of inositol phosphate (IP6). In this study, β-TCP powders were synthesized by mechanochemical method, and the as-prepared powders were heated at 600-1300°C for 1 h. Some properties of the resulting powders were examined. The crystalline phase of the resulting powders in the range of 600-1100°C was of β-TCP single phase. In the cases at 1200°C and 1300°C, the resulting powders were composed of β-TCP and α-TCP. Median particle sizes of the resulting powders increased with heating temperature from 5.35 μm up to 47.7 μm. Dissolution rate of Ca2+ ions from the β-TCP powders was measured by Japanese Industrial Standard T 0330-3. When the heating temperature was at 700°C, the Ca2+ ions solubility was highest among examined ones. The β-TCP powder heated at 700°C for 1 h will be expected as the starting powder for paste-like artificial bone filler with excellent bioresorbability.

scholarly journals Fabrication of Novel Biodegradableα-Tricalcium Phosphate Cement Set by Chelating Capability of Inositol Phosphate and Its Biocompatibility

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Toshiisa Konishi ◽  
Minori Mizumoto ◽  
Michiyo Honda ◽  
Yukiko Horiguchi ◽  
Kazuya Oribe ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Single Phase ◽  
Milling Time ◽  
Inositol Phosphate ◽  
Pure Water ◽  
Tartrate Resistant Acid Phosphatase ◽  
Setting Mechanism ◽  
Tcp Phase

Biodegradableα-tricalcium phosphate (α-TCP) cement based on the chelate-setting mechanism of inositol phosphate (IP6) was developed. This paper examined the effect of the milling time ofα-TCP powder on the material properties of the cement. In addition, biocompatibility of the result cementin vitrousing osteoblasts andin vivousing rabbit models will be studied as well. Theα-TCP powders were ballmilled using ZrO2beads in pure water for various durations up to 270 minutes, with a single-phaseα-TCP obtained at ballmilling for 120 minutes. The resulting cement was mostly composed ofα-TCP phase, and the compressive strength of the cement was8.5±1.1 MPa, which suggested that the cements set with keeping the crystallite phase of starting cement powder. The cell-culture test indicated that the resulting cements were biocompatible materials.In vivostudies showed that the newly formed bones increased with milling time at a slight distance from the cement specimens and grew mature at 24 weeks, and the surface of the cement was resorbed by tartrate-resistant acid phosphatase-(TRAP-)positive osteoclast-like cells until 24 weeks of implantation. The presentα-TCP cement is promising for application as a novel paste-like artificial bone with biodegradability and osteoconductivity.

Preparation of α-Tricalcium Phosphate Powders Surface-Modified with Inositol Phosphate for Cement Fabrication

2014 ◽  
Vol 631 ◽  
pp. 113-118
Author(s):  
Toshiisa Konishi ◽  
Michiyo Honda ◽  
Tomohiko Yoshioka ◽  
Satoshi Hayakawa ◽  
Mamoru Aizawa
Keyword(s):  
Ball Milling ◽  
Tricalcium Phosphate ◽  
Single Phase ◽  
Inositol Phosphate ◽  
Potential Candidate ◽  
Preparation Process ◽  
The Novel ◽  
Powder Preparation ◽  
Setting Mechanism ◽  
Surface Modified

We have previously developed biodegradable β-tricalcium phosphate (β-TCP) cement based on the chelate-setting mechanism of inositol phosphate (IP6). The β-TCP cement powder for the cement fabrication was prepared via a novel powder preparation process, in which the starting β-TCP powders were prepared by simultaneous ball-milling and surface-modification in the IP6 solution. In the present study, the novel powder preparation process was applied to an α-TCP powder, and effect of milling time and beads size for ball-milling on the material properties of the α-TCP powders was investigated. The α-TCP powder ball-milled in 1000 ppm IP6 solution for 4 h with 2 mm-diameter beads was composed of single phase α-TCP with the smallest particle size of 2.2 µm. Dissolution of 4 h-milled α-TCP powder was approximately twice higher than that of starting α-TCP powder before ball-milling. The α-TCP powder with high dissolution property prepared via the novel powder preparation process is potential candidate for fabrication of the chelate-setting cement.

Fabrication and Characterization of Chelate-Setting β-Tricalcium Phosphate Cements with Enhanced Bioresorbability

2016 ◽  
Vol 720 ◽  
pp. 157-161
Author(s):  
Kohei Nagata ◽  
Toshiisa Konishi ◽  
Michiyo Honda ◽  
Mamoru Aizawa
Keyword(s):  
Dissolution Rate ◽  
Ball Milling ◽  
Tricalcium Phosphate ◽  
Inositol Phosphate ◽  
Pure Water ◽  
High Specific Surface Area ◽  
Cement Pastes ◽  
Sodium Hydrogen Phosphate ◽  
Japanese Industrial Standard ◽  
Human Cancellous Bone

A novel chelate-setting β-tricalcium phosphate (β-TCP) cement with anti-washout properties have been fabricated previously. This cement has been set on the basis of chelating ability of inositol phosphate (IP6). In this study, the ball-milling and surface-modification conditions of starting β-TCP cement powders were optimized in terms of bioresorbability. Starting powders were prepared by simultaneously ball-milling at 300 rpm for 3 h with 1 mm diameter ZrO2 beads and surface-modifying with 40 cm3 of 3000 ppm IP6 solution. The resulting starting powder was consisted of β-TCP single phase, and had high specific surface area of 48.3 m2∙g-1. Cement pastes were prepared by mixing the starting powder and the aqueous solution composed of 2.5 mass% sodium hydrogen phosphate, 1.5 mass% citric acid and 1.0 mass% sodium alginate at a powder/liquid ratio of 1/0.90 [g∙cm-3] for 2 min. After setting in pure water for 72 h, compressive strength of the cement specimens was higher than that of human cancellous bone. Dissolution rate of Ca2+ ions was measured by according to Japanese Industrial Standard T 0330-3. The results of Ca2+ ions dissolution rate test demonstrated that the cement specimens derived from the above starting powder were the highest dissolution rate among examined ones. This cement would be expected as bone fillers with high bioresobability.

Fabrication of βTCP with Fully-Interconnected Porous Structure

2011 ◽  
Vol 493-494 ◽  
pp. 135-138
Author(s):  
Taro Nikaido ◽  
Kanji Tsuru ◽  
Fumikazu Daitou ◽  
Melvin L. Munar ◽  
Shigeki Matsuya ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Porous Structure ◽  
Tricalcium Phosphate ◽  
Single Phase ◽  
Sintering Temperature ◽  
Crystal Phase ◽  
Dicalcium Phosphate Dihydrate ◽  
Bone Filler ◽  
Phosphate Dihydrate ◽  
Significant Difference

Calcium phosphate foam could be an ideal bone filler and scaffold for tissue engineering. This paper describes fabrication method of β-tricalcium phosphate (βTCP) foam with fully-interconnected porous structure by employing magnesium oxide (MgO) as βTCP stabilizer. The foam was prepared using the so-called ceramics foam method. MgO was added to calcium carbonate and dicalcium phosphate dihydrate so that 0, 1, 2, 3, 4, 6 and 8 mol% calcium would be substituted by magnesium (Mg) in βTCP structure. After sintering at 1500°C, crystal phase of the obtained foam included α-tricalcium phosphate (αTCP) when no Mgor less than 3 mol% Mg was added. In contrast, crystal phase was single phase βTCP when 3 mol% or higher Mg was added. The compressive strength was approximately 15 kPa and the porosity was above 95% for all specimens. No significant difference was observed between αTCP and βTCP foams in compressive strength and porosity when the sintering temperature was the same.

Comparative Study on Bioresorbability of Chelate-Setting Cements with Various Calcium-Phosphate Phase Using Rabbit Model

2012 ◽  
Vol 529-530 ◽  
pp. 167-172 ◽  
Author(s):  
Toshiisa Konishi ◽  
Shuhei Takahashi ◽  
Minori Mizumoto ◽  
Michiyo Honda ◽  
Koki Kida ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Inositol Phosphate ◽  
Rabbit Model ◽  
The Novel ◽  
Calcium Phosphate Cements ◽  
Phosphate Phase ◽  
Setting Mechanism ◽  
Calcium Phosphate Phase

We have developed novel calcium-phosphate cements (CPCs) based on the chelate-setting mechanism of inositol phosphate (IP6) using hydroxyapatite (HAp), β-tricalcium phosphate (β-TCP) and α-TCP as starting materials. These cements (IP6-HAp, IP6-β-TCP and IP6-α-TCP cements) have different bioresorbability due to the chemical composition of starting materials. In the present study, biocompatibility and bioresorbability of the above three cements and commercially available cement (Biopex®-R) was histologically evaluated in vivo using rabbit model for 4, 8, and 24 weeks, in addition to their dissolution in vitro. The dissolution of these cements increased in the order of IP6-HAp, IP6-β-TCP and IP6-α-TCP cements. The newly-formed bones were directly in contact with both the IP6-HAp and Biopex®-R cement specimens. As for the IP6-β-TCP and IP6-α-TCP cements, newly-formed bones were formed time-dependently slightly apart from the cement specimens. Resorption rate for Biopex®-R, IP6-HAp, IP6-β-TCP, and IP6-α-TCP cements after 24 weeks implantation were of 7.2, 5.0, 13.7, and 16.2%, respectively, compared to original cements. The present chelate-setting cements with different bioresorbability are promising candidates for application as the novel CPCs.

Fabrication of novel bioresorbable β-tricalcium phosphate cement on the basis of chelate-setting mechanism of inositol phosphate and its evaluation

2011 ◽  
Vol 119 (1385) ◽  
pp. 35-42 ◽  
Author(s):  
Shuhei TAKAHASHI ◽  
Toshiisa KONISHI ◽  
Koji NISHIYAMA ◽  
Minori MIZUMOTO ◽  
Michiyo HONDA ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Inositol Phosphate ◽  
Setting Mechanism

Biodegradable β-tricalcium phosphate cement with anti-washout property based on chelate-setting mechanism of inositol phosphate

2013 ◽  
Vol 24 (6) ◽  
pp. 1383-1394 ◽  
Author(s):  
Toshiisa Konishi ◽  
Shuhei Takahashi ◽  
Zhi Zhuang ◽  
Kohei Nagata ◽  
Minori Mizumoto ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Inositol Phosphate ◽  
Setting Mechanism

Characterization of Three Calcium Phosphate Microporous Granulated Bioceramics

2014 ◽  
Vol 936 ◽  
pp. 687-694 ◽  
Author(s):  
Nelson H.A. Camargo ◽  
Priscila F. Franczak ◽  
Enori Gemelli ◽  
Bruna Ditzel da Costa ◽  
Aury Nunes de Moraes
Keyword(s):  
Controlled Release ◽  
Calcium Phosphate ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Bone Structure ◽  
Calcium Phosphates ◽  
Morphological Characteristics ◽  
Biological Environment

The calcium phosphate microporous bioceramics, and hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) biphasic compositions, in the granular form of microporous biomaterials, are research themes and present potential biomedical applications in rebuilding and repairing maxillofacial bone and tooth structure and in orthopedic applications. This is associated with microstructural characteristics of biocompatibility and bioactivity and osteoconductivity properties that these biomaterials offer when appliedin vivoor in simulated environment. Another differential point of these biomaterials is the solubilization capacity that they present when applied in the biological environment. These compositions of calcium phosphates (hydroxyapatite matrix and/or β-tricalcium phosphate) allow for the gradual release of calcium and phosphate ions for the biological environment, which are absorbed and promote the formation of new bone tissue. These materials are also promising in applications in the field of traumatology as in the repair of traumatized bone tissue and drugs controlled release and bone structure treatments. The favorable results of these biomaterials as bone reconstruction matrix and drugs controlled release are associated with crystallographic characteristics, morphology, surface and solubility that these biomaterials present when in contact with body fluids. This work aimed to describe three types of calcium phosphate microporous granulated biomaterials. The biomaterials used were provided by the Biomaterials Group from Universidade do Estado de Santa Catarina - UDESC and are: hydroxyapatite, β-tricalcium phosphate and biphasic composition 60% hydroxyapatite/40% β-tricalcium phosphate. The Scanning Electron Microscopy technique (SEM) was used for carrying out the morphological characterization and microstructure studies of granulated biomaterials. The X-Ray Diffractometry (XRD) served for characterization of crystalline phases. Arthur Method was used for determining open porosity and hydrostatic density of biomaterials. The BET technique served to support determination of the surface area of microporous granulated biomaterials. The results are encouraging and show that these biomaterials present promising morphological characteristics and microporous microstructure as wettability and capillarity. These characteristics may contribute to biomaterial osteointegration by new tissue, bone formation and mineralization process.

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