Genomic DNA extraction and amplification of Leishmania donovani using polymerase chain reaction (PCR) from archived, Giemsa- stained slides

Neospora caninum is a coccidian parasite causing abortion in cattle and neurological problems in horses. Dogs are definitive hosts of N. caninum. Polymerase chain reaction is the most specific method used for the detection of N. caninum oocytes. In the present study, a total of 100 fecal samples were collected from naturally infected dogs. Of the 100 samples analyzed, 11 of them were detected with Hammondia/Neospora-like oocytes. Genomic DNA was isolated using a commercially available DNA extraction kit. The Nc5 gene specific to N. caninum was amplified by PCR and two of the eleven samples with Hammondia/Neospora-like oocytes formed ~337 bp repeatable band. In conclusion, N. caninum, which has been shown to cause neurological disorders in dogs and to abortion in cattle, was detected in naturally infected dogs in Van Province in Polymerase chain reaction.

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Comparison of three DNA extraction methods for polymerase chain reaction (PCR) analysis of bacterial genomic DNA

African Journal of Microbiology Research ◽

10.5897/ajmr2013.6459 ◽

2014 ◽

Vol 8 (6) ◽

pp. 598-602 ◽

Cited By ~ 20

Author(s):

B. Ahmed Omar ◽

H. Asghar Atif ◽

M. Elhassan Mogahid

Keyword(s):

Polymerase Chain Reaction ◽

Dna Extraction ◽

Genomic Dna ◽

Extraction Methods ◽

Pcr Analysis ◽

Chain Reaction ◽

Polymerase Chain ◽

Dna Extraction Methods

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Use of Multiple Displacement Amplification as Pre-polymerase Chain Reaction (Pre-PCR) to amplify genomic DNA of siphonapterids preserved for long periods in scientific collections

Parasites & Vectors ◽

10.1186/1756-3305-3-86 ◽

2010 ◽

Vol 3 (1) ◽

pp. 86 ◽

Cited By ~ 5

Author(s):

Daniel M Avelar ◽

Pedro M Linardi

Keyword(s):

Polymerase Chain Reaction ◽

Genomic Dna ◽

Multiple Displacement Amplification ◽

Chain Reaction ◽

Polymerase Chain ◽

Scientific Collections

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Use of the polymerase chain reaction for detection of Fusarium graminearum in bulgur wheat

Food Science and Technology ◽

10.1590/s0101-20612012005000027 ◽

2012 ◽

Vol 32 (1) ◽

pp. 201-208 ◽

Cited By ~ 1

Author(s):

Carla Bertechini Faria ◽

Giovana Caputo Almeida-Ferreira ◽

Karina Bertechine Gagliardi ◽

Tatiane Cristina Albuquerque Alves ◽

Dauri José Tessmann ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Fusarium Graminearum ◽

Genomic Dna ◽

Solid Medium ◽

Food Contamination ◽

Chain Reaction ◽

Specific Pcr ◽

Culture Characteristics ◽

Mycotoxigenic Fungi ◽

Polymerase Chain

The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific PCR reaction. The F. graminearum species was detected only in samples that were heavily contaminated. In the second method, samples of bulgur wheat were inoculated on a solid medium, and isolates having F. graminearum culture characteristics were obtained. The DNA extracted from these isolates was tested in F. graminearum specific PCR reactions. An isolate obtained had its trichothecene genotype identified by PCR. The established methodology could be used in surveys of food contamination with F. graminearum.

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