Molecular Detection of Neospora caninum from Naturally Infected Dogs in Van Province, East Turkey

Neospora caninum is a coccidian parasite causing abortion in cattle and neurological problems in horses. Dogs are definitive hosts of N. caninum. Polymerase chain reaction is the most specific method used for the detection of N. caninum oocytes. In the present study, a total of 100 fecal samples were collected from naturally infected dogs. Of the 100 samples analyzed, 11 of them were detected with Hammondia/Neospora-like oocytes. Genomic DNA was isolated using a commercially available DNA extraction kit. The Nc5 gene specific to N. caninum was amplified by PCR and two of the eleven samples with Hammondia/Neospora-like oocytes formed ~337 bp repeatable band. In conclusion, N. caninum, which has been shown to cause neurological disorders in dogs and to abortion in cattle, was detected in naturally infected dogs in Van Province in Polymerase chain reaction.

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Genomic DNA extraction and amplification of Leishmania donovani using polymerase chain reaction (PCR) from archived, Giemsa- stained slides

Sri Lankan Journal of Infectious Diseases ◽

10.4038/sljid.v11i1.8323 ◽

2021 ◽

Vol 11 (1) ◽

pp. 23

Author(s):

A. Amarasinghe ◽

D. Iddawela

Keyword(s):

Polymerase Chain Reaction ◽

Dna Extraction ◽

Genomic Dna ◽

Leishmania Donovani ◽

Chain Reaction ◽

Genomic Dna Extraction ◽

Polymerase Chain

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Molecular detection and occurrence of equine theileriosis in Arabian horses in Al-Najaf province/Iraq

Brazilian Journal of Veterinary Research and Animal Science ◽

10.11606/issn.1678-4456.bjvras.2020.166996 ◽

2020 ◽

Vol 57 (3) ◽

pp. e166996

Author(s):

Hayder Mohammad Al-Rammahi ◽

Abdulameer Abed Hatem ◽

Asaad Chasib Al-Atabi

Keyword(s):

Polymerase Chain Reaction ◽

Dna Extraction ◽

Molecular Detection ◽

Molecular Technique ◽

Chain Reaction ◽

Blood Samples ◽

Equine Piroplasmosis ◽

Polymerase Chain

This study was designed to detect equine piroplasmosis using the molecular technique in Al-Najaf province during the season that showed an increment in tick activities. Blood samples were collected from 110 horses with more than two signs of piroplasmosis. After DNA extraction, the product was examined by a polymerase chain reaction to amplify 18SrRNA. The results showed that the overall percentage of equine theileriosis was 38.18%. According to gender, the percentage of infection was 43.48% and 29.27% in females and males, respectively. Significant variations appeared between infected horses according to age, and the percentage of infection was 50% and 35.22% in less than 2 years and more than 2 years age, respectively. Moreover, the percentage of infection was 62.5% and 19.35% in animals with and without acariasis, respectively. Significant variations were also seen in equine theileriosis according to geographical areas, and the higher percentage was reported in Hera district (60.87%), while the lowest percentage was in the center of Al-Najaf (21.43%). This difference may be due to different distribution of vector of disease (tick), which may be the availability of the suitable weather that helped in the multiplication of the intermediate vectors. In conclusion, this study proved the variations in the occurrences of equine piroplasmosis according to gender, age, and geographical areas.

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