Genomic DNA Extraction from Ferula jaeschkeana Vatke (Apiaceae) Optimized for Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) analysis

AbstractThe random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique was applied to two closely-related echinostome species, Echinostoma trivolvis and E. caproni, to demonstrate interspecffic polymorphisms of genomic DNA. Band patterns generated using five individual primers showed that these two echinostomes were genetically distinct, although they share genomic DNA to some extent.

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Differentiation ofAeromonasgenomospecies using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

Journal of Applied Bacteriology ◽

10.1111/j.1365-2672.1996.tb03235.x ◽

1996 ◽

Vol 80 (4) ◽

pp. 402-410 ◽

Cited By ~ 18

Author(s):

H.J. Oakey ◽

J.T. Ellis ◽

L.F. Gibson

Keyword(s):

Polymerase Chain Reaction ◽

Dna Polymerase ◽

Random Amplified Polymorphic Dna ◽

Chain Reaction ◽

Rapd Pcr ◽

Polymerase Chain

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Genetic variability of Brazilian Toxoplasma gondii strains detected by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and simple sequence repeat anchored-PCR (SSR-PCR)

Infection Genetics and Evolution ◽

10.1016/j.meegid.2004.03.002 ◽

2004 ◽

Vol 4 (2) ◽

pp. 131-142 ◽

Cited By ~ 36

Author(s):

A FERREIRA ◽

R VITOR ◽

A CARNEIRO ◽

G BRANDAO ◽

M MELO

Keyword(s):

Polymerase Chain Reaction ◽

Dna Polymerase ◽

Simple Sequence Repeat ◽

Random Amplified Polymorphic Dna ◽

Sequence Repeat ◽

Chain Reaction ◽

Rapd Pcr ◽

Anchored Pcr ◽

Polymerase Chain ◽

Simple Sequence

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Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR)

Molecular Toxicology Protocols - Methods in Molecular Biology ◽

10.1007/978-1-62703-739-6_6 ◽

2014 ◽

pp. 71-81 ◽

Cited By ~ 3

Author(s):

Kamaleshwar P. Singh

Keyword(s):

Dna Methylation ◽

Polymerase Chain Reaction ◽

Dna Polymerase ◽

Random Amplified Polymorphic Dna ◽

Chain Reaction ◽

Rapd Pcr ◽

Polymerase Chain

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Use of Genetic Polymorphisms Detected by the Random-Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) for Differentiation and Identification of Aedes aegypti Subspecies and Populations

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.1992.47.893 ◽

1992 ◽

Vol 47 (6) ◽

pp. 893-901 ◽

Cited By ~ 111

Author(s):

Mary E. Ballinger-Crabtree ◽

William C. Black ◽

Barry R. Miller

Keyword(s):

Polymerase Chain Reaction ◽

Aedes Aegypti ◽

Genetic Polymorphisms ◽

Dna Polymerase ◽

Random Amplified Polymorphic Dna ◽

Chain Reaction ◽

Rapd Pcr ◽

Polymerase Chain

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DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD–PCR)

The Canadian Entomologist ◽

10.4039/ent131229-2 ◽

1999 ◽

Vol 131 (2) ◽

pp. 229-230 ◽

Cited By ~ 3

Author(s):

C.K. Chan ◽

D.J. Petersen ◽

T.C. Vrain

Keyword(s):

Polymerase Chain Reaction ◽

Dna Polymerase ◽

Acyrthosiphon Pisum ◽

Random Amplified Polymorphic Dna ◽

Aphis Gossypii ◽

Aphis Fabae ◽

Chain Reaction ◽

Rapd Pcr ◽

Laboratory Colonies ◽

Polymerase Chain

Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namely Acyrthosiphon pisum (Harris), Aphis fabae Scopoli, Aphis frangulae group, and Aphis gossypii Glover.

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