scholarly journals Bacillus anthracisToxins Inhibit Human Neutrophil NADPH Oxidase Activity

2006 ◽  
Vol 176 (12) ◽  
pp. 7557-7565 ◽  
Author(s):  
Matthew A. Crawford ◽  
Caroline V. Aylott ◽  
Raymond W. Bourdeau ◽  
Gary M. Bokoch
Keyword(s):  
Nadph Oxidase ◽  
Human Neutrophil ◽  
Oxidase Activity ◽  
Nadph Oxidase Activity

scholarly journals Relation of human neutrophil phorbol ester receptor occupancy and NADPH- oxidase activity

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 333-339 ◽  
Author(s):  
AI Tauber ◽  
DB Brettler ◽  
EA Kennington ◽  
PM Blumberg
Keyword(s):  
Nadph Oxidase ◽  
Phorbol Ester ◽  
Respiratory Burst ◽  
Human Neutrophil ◽  
Oxidase Activity ◽  
Receptor Occupancy ◽  
Human Neutrophils ◽  
Binding Studies ◽  
Nadph Oxidase Activity ◽  
Phorbol Ester Receptor

Abstract Phorbol esters are potent stimulants of the respiratory burst of the human neutrophil as assessed by superoxide (O2-) generation in whole cells and by NADPH-oxidase activity in a broken-cell 27,000-g particulate fraction. Phorbol 12-myristate, 13-acetate (PMA) and phorbol 12,13-dibutyrate (PDBu) stimulate production of O2- by human neutrophils with ED50 concentrations of 3.9 +/- 2.1 and 41.7 +/- 7.1 nM, respectively. The relation of biologic activity to receptor occupancy was assessed with binding studies of PMA and PDBu. Phorbol ester binding revealed a single high affinity phorbol ester receptor present at 7.6 x 10(5) sites/cell. The binding affinities for PMA and PDBu, 4.9 nM and 38.4 nM, respectively, agreed quantitatively with that of biologic potencies. Because of the high concentration of phorbol ester receptors (up to 125 nM) and the large amount of nonspecific binding at high cell density, apparent discrepancies between ED50′s for NADPH-oxidase and whole cell O2- generation were noted. With the use of low cell concentrations, quantitative agreement between intact cell production of O2-, NADPH-oxidase activity, and receptor binding was found. These results further support the identity of the NADPH-oxidase as the enzymatic source of respiratory burst O2- production in human neutrophils.

scholarly journals Relation of human neutrophil phorbol ester receptor occupancy and NADPH- oxidase activity

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 333-339
Author(s):  
AI Tauber ◽  
DB Brettler ◽  
EA Kennington ◽  
PM Blumberg
Keyword(s):  
Nadph Oxidase ◽  
Phorbol Ester ◽  
Respiratory Burst ◽  
Human Neutrophil ◽  
Oxidase Activity ◽  
Receptor Occupancy ◽  
Human Neutrophils ◽  
Binding Studies ◽  
Nadph Oxidase Activity ◽  
Phorbol Ester Receptor

Phorbol esters are potent stimulants of the respiratory burst of the human neutrophil as assessed by superoxide (O2-) generation in whole cells and by NADPH-oxidase activity in a broken-cell 27,000-g particulate fraction. Phorbol 12-myristate, 13-acetate (PMA) and phorbol 12,13-dibutyrate (PDBu) stimulate production of O2- by human neutrophils with ED50 concentrations of 3.9 +/- 2.1 and 41.7 +/- 7.1 nM, respectively. The relation of biologic activity to receptor occupancy was assessed with binding studies of PMA and PDBu. Phorbol ester binding revealed a single high affinity phorbol ester receptor present at 7.6 x 10(5) sites/cell. The binding affinities for PMA and PDBu, 4.9 nM and 38.4 nM, respectively, agreed quantitatively with that of biologic potencies. Because of the high concentration of phorbol ester receptors (up to 125 nM) and the large amount of nonspecific binding at high cell density, apparent discrepancies between ED50′s for NADPH-oxidase and whole cell O2- generation were noted. With the use of low cell concentrations, quantitative agreement between intact cell production of O2-, NADPH-oxidase activity, and receptor binding was found. These results further support the identity of the NADPH-oxidase as the enzymatic source of respiratory burst O2- production in human neutrophils.

Human neutrophil NADPH-oxidase activity is inhibited by lazaroids

Inflammation ◽  
1996 ◽  
Vol 20 (2) ◽  
pp. 139-150 ◽  
Author(s):  
A. J. Theron ◽  
R. Anderson
Keyword(s):  
Nadph Oxidase ◽  
Human Neutrophil ◽  
Oxidase Activity ◽  
Nadph Oxidase Activity

scholarly journals Neuroprotective Benefits of Exercise and MitoQ on Memory Function, Mitochondrial Dynamics, Oxidative Stress, and Neuroinflammation in D-Galactose-Induced Aging Rats

2021 ◽  
Vol 11 (2) ◽  
pp. 164
Author(s):  
Jae-Hoon Jeong ◽  
Jung-Hoon Koo ◽  
Jang Soo Yook ◽  
Joon-Yong Cho ◽  
Eun-Bum Kang
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Cognitive Impairment ◽  
Nadph Oxidase ◽  
Learning And Memory ◽  
Oxidase Activity ◽  
Mitochondrial Dynamics ◽  
Nadph Oxidase Activity ◽  
Aging Rats ◽  
Positive Effects

Exercise and antioxidants have health benefits that improve cognitive impairment and may act synergistically. In this study, we examined the effects of treadmill exercise (TE) and mitochondria-targeted antioxidant mitoquinone (MitoQ), individually or combined, on learning and memory, mitochondrial dynamics, NADPH oxidase activity, and neuroinflammation and antioxidant activity in the hippocampus of D-galactose-induced aging rats. TE alone and TE combined with MitoQ in aging rats reduced mitochondrial fission factors (Drp1, Fis1) and increased mitochondrial fusion factors (Mfn1, Mfn2, Opa1). These groups also exhibited improved NADPH oxidase activity and antioxidant activity (SOD-2, catalase). TE or MitoQ alone decreased neuroinflammatory response (COX-2, TNF-α), but the suppression was greater with their combination. In addition, aging-increased neuroinflammation in the dentate gyrus was decreased in TE but not MitoQ treatment. Learning and memory tests showed that, contrarily, MitoQ alone demonstrated some similar effects to TE but not a definitive improvement. In conclusion, this study demonstrated that MitoQ exerted some positive effects on aging when used as an isolated treatment, but TE had a more effective role on cognitive impairment, oxidative stress, inflammation, and mitochondria dysfunction. Our findings suggest that the combination of TE and MitoQ exerted no synergistic effects and indicated regular exercise should be the first priority in neuroprotection of age-related cognitive decline.

scholarly journals Regulation of NADPH Oxidase Activity in Phagocytes

2010 ◽  
Vol 285 (43) ◽  
pp. 33197-33208 ◽  
Author(s):  
Franck Debeurme ◽  
Antoine Picciocchi ◽  
Marie-Claire Dagher ◽  
Didier Grunwald ◽  
Sylvain Beaumel ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Oxidase Activity ◽  
Nadph Oxidase Activity
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