scholarly journals DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES

2018 ◽  
Vol 63 (2) ◽  
pp. 3947-3949
Author(s):  
Mahmood Ali Kaykhaei ◽  
Massoud Kaykhaii ◽  
Mohammad Hashemi ◽  
Mona Sargazi
Keyword(s):  
Urine Samples ◽  
Total Iodine ◽  
Dispersive Liquid Liquid Microextraction ◽  
Iodine In Urine ◽  
Liquid Microextraction

Dispersive Liquid-Liquid Microextraction Based on Ionic Liquid and Spectrophotometric Determination of Bilirubin in Biological Samples

2020 ◽  
Vol 16 (5) ◽  
pp. 652-659
Author(s):  
Asiye A. Avan ◽  
Hayati Filik
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Biological Samples ◽  
Urine Samples ◽  
Spectrophotometric Detection ◽  
Full Color ◽  
Dispersive Liquid Liquid Microextraction ◽  
Urine And Serum ◽  
Liquid Microextraction

Background: An Ionic Liquid-based based Dispersive Liquid-Liquid Microextraction (IL-DLLME) method was not applied to preconcentration and determination of bilirubin. Ionic Liquids (ILs) are new chemical compounds. In recent years, Ionic Liquids (ILs) have been employed as alternative solvents to toxic organic solvents. Due to these perfect properties, ILs have already been applied in many analytical extraction processes, presenting high extraction yield and selectivity for analytes. Methods: In this study, IL-DLLME was applied to biological samples (urine and serum) for the spectrophotometric detection of bilirubin. For bilirubin analysis, the full-color development was based on the reaction with periodate in the presence of hydrochloric acid. The high affinity of bilirubin for the ionic liquid phase gave extraction percentages above 98% in 0.3 M HCl solution. Results: Several IL-extraction parameters were optimized and room temperature ionic liquid 1-butyl- 1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide and ethanol were used as extraction and disperser solution. The linear range was found in the range of 0.5-6.0 μM (0.3-3.5 μg mL-1) and the limits of detection of the proposed method was 0.5 μM (0.3 μg mL-1). The proposed method was applied for the preconcentration and separation of trace bilirubin in real urine samples. Also, the recoveries for bilirubin in spiked biological samples (urine and serum) were found to be acceptable, between 95-102%. Conclusion: The proposed IL-DLLMEapproach was employed for the enrichment and determination of trace levels of bilirubin in urine samples using NaIO4 as an oxidizing agent and Uv-vis spectrophotometric detection. The periodate oxidation of bilirubin is rapid, effective, selective, and simple to perform. The method contains only HCl, NaOI4, and an anionic surfactant. The method may be useful for economizing in the consumption of reagents in bilirubin determining. The IL-DLLMEmethod ensures a high yield and has a low toxicity no skin sensitization, no mutagenicity and no ecotoxicity in an aquatic environment since only very low quantities of an IL is required. For full-color formation, no any extra auxiliary reagents are required. Besides, the IL-DLLME technique uses a low-cost instrument such as Uv-vis which is present in most of the medical laboratories.

Separation and Determination of Triclosan and Bisphenol A in Water, Beverage, and Urine Samples by Dispersive Liquid–Liquid Microextraction Combined with Capillary Zone Electrophoresis–UV Detection

2013 ◽  
Vol 96 (2) ◽  
pp. 459-465 ◽  
Author(s):  
Huili Wang ◽  
Aina Zhang ◽  
Wenwei Wang ◽  
Minghua Zhang ◽  
Huanqiang Liu ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Capillary Zone Electrophoresis ◽  
Uv Detection ◽  
Extraction Time ◽  
Urine Samples ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Abstract Dispersive liquid–liquid microextraction combined with capillary zone electrophoresis–UV detection was developed for analyzing triclosan (TCS) and bisphenol A (BPA) in water, beverage, and urine samples. The factors influencing microextraction efficiencies, such as the kind and volume of extraction and dispersive solvent, the extraction time, and the salt effect, were optimized. A background electrolyte composed of 8 mM sodium tetraborate at pH 9.8 was used as the running buffer. Detection was performed at 214 nm. Under the optimum conditions (sample volume, 5.0 mL; extraction solvent, tetrachloroethane, 22.0 μL; dispersive solvent, tetrahydrofuran, 1.0 mL; extraction time, fewer than 5 s; and without salt addition), the enrichment factors were 110.2 and 82.0 for TCS and BPA, respectively. The linear range was 0.02–2 μg/mL with correlation coefficients of 0.9966–0.9969. LODs were in the range of 4.0–8.0 ng/mL. The environmental water, beverage, and urine samples (at spiking levels of 0.1 and 0.4 μg/mL) were successfully analyzed by the proposed method; the recoveries compared to previous methods were in the range of 81.2–103.3%. As a result, this method can be successfully applied for the rapid and convenient determination of TCS and BPA in water, beverage, and urine samples.

Ultrasound-assisted dispersive liquid–liquid microextraction followed by GC–MS/MS analysis for the determination of valproic acid in urine samples

Bioanalysis ◽  
2015 ◽  
Vol 7 (19) ◽  
pp. 2451-2459 ◽  
Author(s):  
Rajeev Jain ◽  
Manoj Kumar Gupta ◽  
Abhishek Chauhan ◽  
Vivek Pandey ◽  
Mohana Krishna Reddy Mudiam
Keyword(s):  
Valproic Acid ◽  
Urine Samples ◽  
Ms Analysis ◽  
Ultrasound Assisted ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction
Sign in / Sign up

Export Citation Format

Share Document