Sero-survey of Hepatitis B surface antigen amongst pregnant women attending Infectious Disease Hospital Bayara, Bauchi State, Nigeria

Hepatitis B virus (HBV) continues to cause serious health problems in developing countries. Neonatal infection with HBV, which is often acquired during delivery, carries a high risk resulting in persistent infection. This research aims to detect the prevalence of Hepatitis B surface Antigen (HBsAg) among pregnant women in our location of study. One hundred and eighty (180) sera samples were screened among pregnant women aged 13-49, using standard enzyme-linked immunosorbent assay (ELISA) method. Structured questionnaire were administered to the subjects to obtain demographic and other relevant data. Overall result showed that 31 (17.2%) were found to be positive for HBsAg among the total subjects screened. The highest prevalence was found among those aged 20-29 with 11 (6.1%) seropositivity (x2=7.902; P=0.048). Considering occupational distribution of volunteer subjects, a high prevalence of 12 (6.7%); P<0.05 was recorded among house wives, which shows a measure of significance compared to other women screened. Furthermore, based on various risk factors subjects with history of surgery and use of unsterilized sharp instruments recorded 15 (8.3%) prevalence (P=0.233; P>0.05). How ever, women in their second trimester of pregnancy recorded a higher prevalence of 23 (12.8%):(P=0.080; P<0.05). This study therefore emphasizes the public health importance of HBV among pregnant women and equally suggests that children born to women with Hepatitis B Virus, be closely monitored for infection beyond the one and the half years of age, this also calls for a proper enlightenment on the dangers posed by the virus, while a well designed vaccination schedule is advocated among the general population.

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Discrepant Hepatitis B Surface Antigen Results in Pregnant Women Screened to Identify Hepatitis B Virus Infection

The Journal of Pediatrics ◽

10.1016/j.jpeds.2014.06.043 ◽

2014 ◽

Vol 165 (4) ◽

pp. 773-778 ◽

Cited By ~ 3

Author(s):

Steven L. Veselsky ◽

Tanja Y. Walker ◽

Nancy Fenlon ◽

Chong-Gee Teo ◽

Trudy V. Murphy

Keyword(s):

Hepatitis B Virus ◽

Virus Infection ◽

Hepatitis B ◽

Pregnant Women ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Hepatitis B Virus Infection ◽

B Virus

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Expression Level of Small Envelope Protein in Addition to Sequence Divergence inside Its Major Hydrophilic Region Contributes to More Efficient Surface Antigen Secretion by Hepatitis B Virus Subgenotype D2 than Subgenotype A2

Viruses ◽

10.3390/v12090967 ◽

2020 ◽

Vol 12 (9) ◽

pp. 967

Author(s):

Qianru Wang ◽

Shuwen Fu ◽

Jing Zhang ◽

Quan Yuan ◽

Jisu Li ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Surface Antigen ◽

Envelope Protein ◽

Hepatitis B Surface Antigen ◽

Site Directed Mutagenesis ◽

Enzyme Linked Immunosorbent Assay ◽

S Protein ◽

Huh7 Cells ◽

B Virus

Hepatitis B surface antigen (HBsAg) promotes persistent hepatitis B virus (HBV) infection. It primarily corresponds to small (S) envelope protein secreted as subviral particles. We previously found that genotype D clones expressed less S protein than genotype A clones but showed higher extracellular/intracellular ratio of HBsAg suggesting more efficient secretion. The current study aimed to characterize the underlying mechanism(s) by comparing a subgenotype A2 clone (geno5.4) with a subgenotype D2 clone (geno1.2). Five types of full-length or subgenomic constructs were transfected to Huh7 cells at different dosage. HBsAg was quantified by enzyme linked immunosorbent assay while envelope proteins were detected by Western blot. We found that ratio of extracellular/intracellular HBsAg decreased at increasing amounts of DNA transfected. Conflicting findings from two types of subgenomic construct confirmed stronger secretion inhibitory effect of the genotype D-derived large envelope protein. Chimeric constructs followed by site-directed mutagenesis revealed geno1.2 specific V118/T127 and F161/A168 in the S protein as promoting and inhibitory of HBsAg secretion, respectively. In conclusion, more efficient HBsAg secretion by subgenotype D2 than subgenotype A2 is attributed to lower level of S protein expression in addition to V118 and T127 in S protein, although its F161 and A168 sequences rather reduce HBsAg secretion.

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Development and Technical and Clinical Validation of a Quantitative Enzyme-Linked Immunosorbent Assay for the Detection of Human Antibodies to Hepatitis B Surface Antigen in Recipients of Recombinant Hepatitis B Virus Vaccine

Clinical and Vaccine Immunology ◽

10.1128/cvi.00431-08 ◽

2009 ◽

Vol 16 (8) ◽

pp. 1236-1246 ◽

Cited By ~ 18

Author(s):

Pierre Cambron ◽

Jeanne-Marie Jacquet ◽

Bernard Hoet ◽

Marc Lievens

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Immunosorbent Assay ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Geometric Mean ◽

Enzyme Linked Immunosorbent Assay ◽

Analytical Sensitivity ◽

Limit Of Quantitation ◽

B Virus

ABSTRACT Pending removal from the market of a commercial assay (the AUSAB [Abbott Laboratories] enzyme immunoassay [EIA]) for the determination of antibodies to hepatitis B surface antigen (HBsAg), a new in-house quantitative enzyme-linked immunosorbent assay (ELISA) to measure antibodies against HBsAg (anti-HBs) was developed (anti-HBs in-house). Specific anti-HBs antibodies were sandwiched between the precoated HBsAg ad and ay subtypes purified from plasma from hepatitis B virus (HBV) human carriers and the recombinant HBsAg adw2 subtype tagged with horseradish peroxidase. The assay was calibrated against the 1st International Reference Preparation for anti-hepatitis B immunoglobulin (lot 1977-W1042). Analytical sensitivity and the limit of quantitation were estimated at 0.43 mIU/ml and 2.0 mIU/ml, respectively. Overall reproducibility was 11.86%, and accuracy was estimated to be 94.89%. More than 4,000 samples from seven clinical trials were tested with the anti-HBs in-house assay and compared to results generated with AUSAB EIA and AUSAB radioimmunoassay (RIA). During the technical validation, the anti-HBs in-house assay was compared to the AUSAB RIA as a reference (n = 919). Overall assessment of concordance and Deming's regression analysis were performed. The coefficient of correlation between the AUSAB RIA and anti-HBs in-house assay was 0.9815 with a slope of 0.9187. The overall agreement between anti-HBs in-house and AUSAB RIA was 97.61%, considering the clinical cutoffs at 3.3 mIU/ml and 1.0 mIU/ml for the respective assays. From a clinical perspective, seroprotection rates and anti-HBs geometric mean antibody concentrations for individual studies calculated with either the in-house assay or the reference assays were similar. Conclusions of individual studies were confirmed. The performance characteristics of the in-house assay are acceptable. There is no evidence that use of the new assay would lead to different clinical conclusions from the reference method.

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Evaluation of hepatitis B virus transmission and antiviral therapy among hepatitis B surface antigen-positive pregnant women

Journal of Obstetrics and Gynaecology Research ◽

10.1111/jog.12821 ◽

2015 ◽

Vol 41 (12) ◽

pp. 1870-1876 ◽

Cited By ~ 6

Author(s):

Suda Tekin Koruk ◽

Ayse Batirel ◽

Sukran Kose ◽

Sila Cetin Akhan ◽

Bilgehan Aygen ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Pregnant Women ◽

Antiviral Therapy ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Virus Transmission ◽

B Virus

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Seroprevalence of Hepatitis B (HBsAg) Infection among Pregnant Women Attending Specialist Hospital Yola, Adamawa State, Nigeria

Journal of Applied Life Sciences International ◽

10.9734/jalsi/2021/v24i530237 ◽

2021 ◽

pp. 41-47

Author(s):

Godly Chessed ◽

Kefas Hellamada Kwala ◽

Aminu Innocent Asika ◽

Sati Danjuma

Keyword(s):

Hepatitis B Virus ◽

Blood Transfusion ◽

Hepatitis B ◽

Marital Status ◽

Pregnant Women ◽

Surface Antigen ◽

Educational Level ◽

Hepatitis B Surface Antigen ◽

Public Health Problem ◽

B Virus

Infection due to Hepatitis B virus is a serious public health problem worldwide, in spite of productive vaccine. Pregnant women infected with hepatitis B virus can transmit the infection to their fetuses and newborns. The aim of the study was to determine the seroprevalence of Hepatitis B infection among pregnant women attending antenatal care at Specialist Hospital Yola. A total of three hundred and thirteen (313) pregnant women were randomly selected for this study. Hepatitis B status was determined by the presence of Hepatitis B surface antigen (HBsAg). A structured questionnaire was used for the data collection. Sera from volunteers were collected and tested for hepatitis B surface antigen. Seroprevalence was determined based on age group, educational level, occupation, marital status, religion and history of blood transfusion. The seroprevalence of HBsAg among consented antenatal attendees was 17(5.43%). The study found highest seroprevalence of HBsAg among the pregnant women in the age bracket 26-35 years 52.94%, 52.94% in women with primary school education, 41.18% among the unemployed, 94.12% among the married women, 70.59% among Muslim women, and 76.47% among women that had blood transfusion. The study also revealed that there is a significant relationship between HBsAg and (educational level, marital status) of the study participants (P < 0.05). The Seroprevalence of HBsAg among antenatal attendees at Specialist Hospital Yola, Nigeria, is on the high side. Therefore, early screening of pregnant women for hepatitis at antenatal clinic, would contribute greatly to the timely management of the disease and will prevent possible transmission to their neonates.

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Seroprevalence of Hepatitis B Virus among antenatal clinic attendees in Gamawa Local Government Area, Bauchi State, Nigeria

10.21203/rs.2.10126/v1 ◽

2019 ◽

Author(s):

Garba Umar Mustapha ◽

Abdulrasul Ibrahim ◽

Muhammad Shakir Balogun ◽

Chukwuma David Umeokonkwo ◽

Aisha Indo Mamman

Keyword(s):

Hepatitis B Virus ◽

Local Government ◽

Hepatitis B ◽

Pregnant Women ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Local Government Area ◽

Hbv Infection ◽

Risk Of Death ◽

B Virus

Abstract Background: Hepatitis B is a potentially life-threatening liver infection and a major global health problem. It causes chronic infection and puts people at high risk of death from cirrhosis and liver cancer. WHO estimated 257 million people are living with hepatitis B virus (HBV) infection and in 2015 alone HBV resulted in to 887,000 deaths globally. We determined the prevalence and associated factors of hepatitis B virus infection among Antenatal Care (ANC) attendees in Gamawa Local Government Area, Bauchi State. Methods: We conducted a descriptive cross-sectional, health facility-based study between March and April 2018. We used systematic random sampling technique to recruit 210 pregnant women aged 15-49 years. With a structured questionnaire, we interviewed the respondents and collected blood sample to test for hepatitis B surface antigen. We calculated frequencies, means, proportions, and tested for associations using Epi Info 7.2 and Microsoft Excel. Results: The mean age of respondents was 24.5 ± 6.0 years; 53.3% of whom were younger than 25 years. All were married, 87.1% had no formal education and up to 90.5% were employed. Overall, 6.7% tested positive for HBsAg; women aged ≥35 years had the highest prevalence (10.5%). None with tertiary education tested positive and women married before 18 years had 6.5% prevalence. Conclusions: The prevalence of HBsAg among pregnant women in Gamawa LGA was 6.7% which is quite lower than the national prevalence reported. We recommended improved surveillance of HBV infection and screening of women attending ANC. Keywords: Hepatitis B virus, Hepatitis B Surface antigen, Prevalence, Pregnancy, Health facilities, Hepatitis B

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