scholarly journals Identification of a differentially expressed thymidine kinase gene related to tapping panel dryness syndrome in the rubber tree (Hevea brasiliensis Muell. Arg.) by random amplified polymorphic DNA screening

2010 ◽  
Vol 1 (1) ◽  
pp. 7 ◽  
Author(s):  
Perumal Venkatachalam ◽  
Natesan Geetha ◽  
Padmanabhan Priya ◽  
Arjunan Thulaseedharan
Keyword(s):  
Thymidine Kinase ◽  
Hevea Brasiliensis ◽  
Random Amplified Polymorphic Dna ◽  
Rubber Tree ◽  
Nucleotide Metabolism ◽  
Polymorphic Band ◽  
Predicted Amino Acid Sequence ◽  
Affecting Factors ◽  
Healthy Tree ◽  
Tapping Panel Dryness

Tapping panel dryness (TPD) syndrome is one of the latex yield affecting factors in the rubber tree (Hevea brasiliensis Mull. Arg.). Therefore, identification of a DNA marker will be highly useful for screening progenies in breeding programs. The major goal of this study was to detect genetic variations and/or identification of gene fragments among 37 Hevea clones by the random amplified polymorphic DNA “fingerprinting” technique. Different levels of DNA polymorphism were detected with various primers and a distinct polymorphic band (2.0 kb) was obtained with OPA-17 primer. It was cloned into a plasmid vector for further sequence characterization and the nucleotide sequence shows homology with a novel putative plant thymidine kinase (TK) gene, designated as HbTK (Hevea brasiliensis thymidine kinase; GenBank accession number AY130829). The protein HbTK has 67%, 65%, 64%, and 63% similarity to TK genes of Medicago, Oryza, Arabidopsis, and Lyco- persicon, respectively, and it was highly conserved in all species analyzed. The predicted amino acid sequence contained conserved domains of TK proteins in the C-terminal half. Southern blot analysis indicated that HbTK is one of the members of a small gene family. Northern blot results revealed that the expression of the HbTK gene was up-regulated in mature bark tissues of the healthy tree while it was down-regulated in the TPD-affected one. These results suggest that this gene may play important roles in maintaining active nucleotide metabolism during cell division at the tapped site of bark tissues in the healthy tree under stress (tapping) conditions for normal latex biosynthesis.

Genome-wide identification of lncRNAs, miRNAs, mRNAs, and their regulatory networks involved in tapping panel dryness in rubber tree (Hevea brasiliensis)

2021 ◽  
Author(s):  
Hui Liu ◽  
Yiyu Hu ◽  
Kun Yuan ◽  
Chengtian Feng ◽  
Qiguang He ◽  
...  
Keyword(s):  
Hevea Brasiliensis ◽  
Regulatory Networks ◽  
Functional Characterization ◽  
Rubber Tree ◽  
Healthy Tree ◽  
Rubber Biosynthesis ◽  
Genome Wide ◽  
Tapping Panel Dryness

Abstract Noncoding RNAs (ncRNAs) play pivotal roles in various biological processes in plants. However, the role of ncRNAs in tapping panel dryness (TPD) of rubber tree (Hevea brasiliensis) is largely unknown. Here, the whole transcriptomes of bark tissues from healthy and TPD trees were performed to identify differentially expressed long ncRNAs (DELs), microRNAs/miRNA (DEMs), genes (DEGs), and their regulatory networks involved in TPD. A total of 263 DELs, 174 DEMs, and 1,574 DEGs were identified in the bark of TPD tree compared with that of healthy tree. KEGG analysis revealed that most of the DEGs and targets of DELs and DEMs were mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction. Additionally, the majority of DEGs and DELs related to rubber biosynthesis were down-regulated in TPD trees. Furthermore, 98 DEGs and 44 DELs were targeted by 54 DEMs, 190 DEGs were identified as putative targets of 56 DELs, and two and 44 DELs were predicted as precursors and endogenous target mimics (eTMs) of two and six DEMs, respectively. Based on these, the DEL-DEM-DEG regulatory network involved in TPD was constructed, and 13 hub DELs, three hub DEMs and two hub DEGs were identified. The results provide novel insights into the regulatory roles of ncRNAs underlying TPD and lay a foundation for future functional characterization of lncRNAs, miRNAs, and genes involved in TPD in rubber tree.

scholarly journals Histo- and cytopathology of trunk phloem necrosis, a form of rubber tree (Hevea brasiliensis Müll. Arg.) tapping panel dryness

2011 ◽  
Vol 59 (6) ◽  
pp. 563 ◽  
Author(s):  
Elisabeth de Faÿ
Keyword(s):  
Hevea Brasiliensis ◽  
Plant Stress ◽  
Rubber Tree ◽  
Structural Features ◽  
Transmission Electron ◽  
Parenchyma Cells ◽  
Microscopic Features ◽  
Phloem Necrosis ◽  
Cyanide Metabolism ◽  
Tapping Panel Dryness

Trunk phloem necrosis (TPN) is a physiological disease of rubber tree (Hevea brasiliensis Müll. Arg.) discovered in the 1980s. It has been distinguished from rubber tree tapping panel dryness (TPD) by its macroscopic symptoms and presumed origin. But little attention has been paid to its microscopic features, and there is now some evidence that both syndromes could be linked to an impaired cyanide metabolism. In order to characterise TPN and compare it with TPD microscopically, the inner phloem of tapping panels was investigated by light and transmission electron microscopy in healthy trees and TPN-affected trees. TPN-affected phloem presented numerous and varied structural and ultrastructural features. There were signs of cellular deterioration in a great number of specialised cells, i.e. laticifers and sieve tubes, and not very specialised cells, i.e. parenchyma cells and companion cells. There were also signs of cellular dedifferentiation in other parenchymatous cells, e.g. in tylosoids and hyperplasic cells. These cells were derived from parenchyma cells that ensheath laticifers in which the latex coagulated. Numerous structural features of TPN are common to TPD, notably tylosoids associated with in situ coagulated latex, which are also known to be early structural markers of TPD and cyanide-induced. It is therefore concluded that TPN is identical to or a variant of TPD, and is a degenerative disease of rubber tree trunk phloem resembling plant stress response, programmed cell death and plant tumourigenesis in some aspects.

Involvement of HbMC1-mediated cell death in tapping panel dryness of rubber tree (Hevea brasiliensis)

2018 ◽  
Vol 39 (3) ◽  
pp. 391-403 ◽  
Author(s):  
Hui Liu ◽  
Yongxuan Wei ◽  
Zhi Deng ◽  
Hong Yang ◽  
Longjun Dai ◽  
...  
Keyword(s):  
Cell Death ◽  
Hevea Brasiliensis ◽  
Rubber Tree ◽  
Tapping Panel Dryness

EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SUNITA BORDE ◽  
ASAWARI FARTADE ◽  
AMOL THOSAR ◽  
RAHUL KHAWAL
Keyword(s):  
Fresh Water ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Genomic Variation ◽  
Polymorphic Band ◽  
Rapd Profile ◽  
Band Size ◽  
Pcr Product ◽  
The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

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