scholarly journals Trends in the use of tissue culture, applications and future aspects

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Baan Munim Twaij ◽  
Zena H. Jazar ◽  
Md. Nazmul Hasan
Keyword(s):  
Tissue Culture ◽  
Secondary Metabolites ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Biological Activities ◽  
Significant Advance ◽  
Scale Production ◽  
Culture Techniques ◽  
Plant Culture ◽  
Culture Development

Plant tissue culture has developed widely incorporated into biotechnology, the agricultural systems being a key factor to support many pharmaceutical and industrial outcomes. Since 1902 there is vast progress in plant culture and its application has emerged having great diversity in the science filed.  Due to development and desire to grow on high scale production in the past few decades, tissue culture techniques were manipulated for improvement of plant growth, biological activities, transformation, and secondary metabolites production. A significant advance in techniques has been sought to deal with problems of low concentrations of secondary metabolites in whole plants. The augmented use of plant culture is due to a superior perceptive of plant oriented compounds and secondary metabolites from economically important plants. Due to development in modern techniques, several particular protocols have been developed for the production of a wide array of secondary metabolites of plants on a commercial scale. Plant tissue culture has to lead to significant contributions in recent times and today they constitute an indispensable tool in the advancement of agricultural sciences and modern agriculture. This review would enable us to have an analysis of plant tissue culture development for agriculture, human health and wellbeing in general.

scholarly journals ANALYSIS OF SECONDARY METABOLITES OF CALLUS OF RAMBUTAN Nephelium lappaceum L

Agric ◽  
2021 ◽  
Vol 33 (1) ◽  
pp. 13-22
Author(s):  
Fahrauk Faramayuda ◽  
Elfahmi Elfahmi ◽  
Weni Widy Astuti
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Metabolites ◽  
Culture Techniques ◽  
Solid Media ◽  
New Information ◽  
Nephelium Lappaceum ◽  
The Right ◽  
Culture Development

Rambutan plant (Nephelium lappaceum L.) is a member of the Sapindaceae family. The rambutan plant is one of the natural ingredients that can be developed as traditional medicine. Rambutan peel has the potential for good antioxidant and anticancer activity. Rambutan fruit does not grow every time it needs efforts to produce the active substance in rambutan, using plant tissue culture techniques. The use of the correct variety of mediums and hormones at the right concentration is the key to thriving tissue culture. Explants derived from rambutan leaves were planted precisely on solid media Murashige and Skoog (MS) and WoddyPlant Medium (WPM) containing Indole-3-Butyric Acid (IBA) and Kinetin. After seven days, the callus was subcultured, then after 35 days, the subculture callus was collected and dried. Dry callus and rambutan leaves (Wild type) were macerated with n-hexane, ethyl acetate, and ethanol. The concentrated extract was then applied to a GF 254 silica gel plate with the mobile phase Toluene-Acetone (7: 3) and n-hexane-EthylAsetate (3: 7). The results showed that the concentration of IBA 2 ppm and kinetin three ppm was the best combination because it produced callus. TLC results of rambutan leave with plant tissue culture containing flavonoids and triterpenoids. This study provides new information regarding the induction of rambutan callus and can become the basis for producing active metabolites in rambutan with cell suspension culture development.  

scholarly journals Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

2022 ◽  
Vol 12 (2) ◽  
pp. 838
Author(s):  
Maulidia Rahmawati ◽  
Choirul Mahfud ◽  
Gianfranco Risuleo ◽  
Nurul Jadid
Keyword(s):  
Tissue Culture ◽  
Silver Nanoparticles ◽  
Secondary Metabolites ◽  
Animal Welfare ◽  
Secondary Metabolite ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Well Being ◽  
Therapeutic Agents ◽  
Scale Production

Plant tissue culture plays an important role in plant biotechnology due to its potential for massive production of improved crop varieties and high yield of important secondary metabolites. Several efforts have been made to ameliorate the effectiveness and production of plant tissue culture, using biotic and abiotic factors. Nowadays, the addition of nanoparticles as elicitors has, for instance, gained worldwide interest because of its success in microbial decontamination and enhancement of secondary metabolites. Nanoparticles are entities in the nanometric dimension range: they possess unique physicochemical properties. Among all nanoparticles, silver-nanoparticles (AgNPs) are well-known for their antimicrobial and hormetic effects, which in appropriate doses, led to the improvement of plant biomass as well as secondary metabolite accumulation. This review is focused on the evaluation of the integration of nanotechnology with plant tissue culture. The highlight is especially conveyed on secondary metabolite enhancement, effects on plant growth and biomass accumulation as well as their possible mechanism of action. In addition, some perspectives of the use of nanomaterials as potential therapeutic agents are also discussed. Thus, the information provided will be a good tool for future research in plant improvement and the large-scale production of important secondary metabolites. Elicitation of silver-nanoparticles, as well as nanomaterials, function as therapeutic agents for animal well-being is expected to play a major role in the process. However, nanosized supramolecular aggregates have received an increased resonance also in other fields of application such as animal welfare. Therefore, the concluding section of this contribution is dedicated to the description and possible potential and usage of different nanoparticles that have been the object of work and expertise also in our laboratories.

Plant tissue culture techniques—Tools in plant micropropagation

2011 ◽  
Vol 22 ◽  
pp. S130 ◽  
Author(s):  
Ahmet Onay ◽  
Hakan Yildirim ◽  
Yelda Ozden Tokatli ◽  
Hulya Akdemir ◽  
Veysel Suzerer
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Culture Techniques ◽  
Plant Micropropagation ◽  
Tissue Culture Techniques

Biotechnology and the Industrialization of Horticulture in India

2002 ◽  
Vol 31 (3) ◽  
pp. 187-192 ◽  
Author(s):  
C. Raghava Reddy ◽  
E. Haribabu
Keyword(s):  
Tissue Culture ◽  
Plant Breeding ◽  
Production Process ◽  
Multinational Corporations ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Manufacturing Industry ◽  
Division Of Labour ◽  
Culture Techniques ◽  
Breeding Techniques

This paper delineates changes in the organization of the production of horticultural plants as a result of the introduction of plant tissue culture techniques in India. Conventionally, horticultural plantlets have been produced in farmer-managed nurseries by using traditional plant breeding techniques such as grafting, budding, layering, seed propagation, etc. Over several centuries, the production process was organized as a craft, based on empirical experience. During the last decade, many multinational corporations and large Indian industrial companies have made substantial investments in horticulture by deploying tissue culture. In a comparative study of nurseries using conventional plant breeding techniques and plant tissue culture, it was observed that production processes had undergone several changes as a result of the introduction of tissue culture. In traditional nurseries the production process was organized according to the simple division of labour. In contrast, plant tissue culture technology was introduced within a complex organizational structure with a formal hierarchy similar to that of the manufacturing industry. Plant tissue culture has ushered in the industrialization of horticulture.

Sign in / Sign up

Export Citation Format

Share Document