scholarly journals ANALYSIS OF SECONDARY METABOLITES OF CALLUS OF RAMBUTAN Nephelium lappaceum L

Agric ◽  
2021 ◽  
Vol 33 (1) ◽  
pp. 13-22
Author(s):  
Fahrauk Faramayuda ◽  
Elfahmi Elfahmi ◽  
Weni Widy Astuti
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Metabolites ◽  
Culture Techniques ◽  
Solid Media ◽  
New Information ◽  
Nephelium Lappaceum ◽  
The Right ◽  
Culture Development

Rambutan plant (Nephelium lappaceum L.) is a member of the Sapindaceae family. The rambutan plant is one of the natural ingredients that can be developed as traditional medicine. Rambutan peel has the potential for good antioxidant and anticancer activity. Rambutan fruit does not grow every time it needs efforts to produce the active substance in rambutan, using plant tissue culture techniques. The use of the correct variety of mediums and hormones at the right concentration is the key to thriving tissue culture. Explants derived from rambutan leaves were planted precisely on solid media Murashige and Skoog (MS) and WoddyPlant Medium (WPM) containing Indole-3-Butyric Acid (IBA) and Kinetin. After seven days, the callus was subcultured, then after 35 days, the subculture callus was collected and dried. Dry callus and rambutan leaves (Wild type) were macerated with n-hexane, ethyl acetate, and ethanol. The concentrated extract was then applied to a GF 254 silica gel plate with the mobile phase Toluene-Acetone (7: 3) and n-hexane-EthylAsetate (3: 7). The results showed that the concentration of IBA 2 ppm and kinetin three ppm was the best combination because it produced callus. TLC results of rambutan leave with plant tissue culture containing flavonoids and triterpenoids. This study provides new information regarding the induction of rambutan callus and can become the basis for producing active metabolites in rambutan with cell suspension culture development.  

scholarly journals Trends in the use of tissue culture, applications and future aspects

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Baan Munim Twaij ◽  
Zena H. Jazar ◽  
Md. Nazmul Hasan
Keyword(s):  
Tissue Culture ◽  
Secondary Metabolites ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Biological Activities ◽  
Significant Advance ◽  
Scale Production ◽  
Culture Techniques ◽  
Plant Culture ◽  
Culture Development

Plant tissue culture has developed widely incorporated into biotechnology, the agricultural systems being a key factor to support many pharmaceutical and industrial outcomes. Since 1902 there is vast progress in plant culture and its application has emerged having great diversity in the science filed.  Due to development and desire to grow on high scale production in the past few decades, tissue culture techniques were manipulated for improvement of plant growth, biological activities, transformation, and secondary metabolites production. A significant advance in techniques has been sought to deal with problems of low concentrations of secondary metabolites in whole plants. The augmented use of plant culture is due to a superior perceptive of plant oriented compounds and secondary metabolites from economically important plants. Due to development in modern techniques, several particular protocols have been developed for the production of a wide array of secondary metabolites of plants on a commercial scale. Plant tissue culture has to lead to significant contributions in recent times and today they constitute an indispensable tool in the advancement of agricultural sciences and modern agriculture. This review would enable us to have an analysis of plant tissue culture development for agriculture, human health and wellbeing in general.

Plant tissue culture techniques—Tools in plant micropropagation

2011 ◽  
Vol 22 ◽  
pp. S130 ◽  
Author(s):  
Ahmet Onay ◽  
Hakan Yildirim ◽  
Yelda Ozden Tokatli ◽  
Hulya Akdemir ◽  
Veysel Suzerer
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Culture Techniques ◽  
Plant Micropropagation ◽  
Tissue Culture Techniques

Biotechnology and the Industrialization of Horticulture in India

2002 ◽  
Vol 31 (3) ◽  
pp. 187-192 ◽  
Author(s):  
C. Raghava Reddy ◽  
E. Haribabu
Keyword(s):  
Tissue Culture ◽  
Plant Breeding ◽  
Production Process ◽  
Multinational Corporations ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Manufacturing Industry ◽  
Division Of Labour ◽  
Culture Techniques ◽  
Breeding Techniques

This paper delineates changes in the organization of the production of horticultural plants as a result of the introduction of plant tissue culture techniques in India. Conventionally, horticultural plantlets have been produced in farmer-managed nurseries by using traditional plant breeding techniques such as grafting, budding, layering, seed propagation, etc. Over several centuries, the production process was organized as a craft, based on empirical experience. During the last decade, many multinational corporations and large Indian industrial companies have made substantial investments in horticulture by deploying tissue culture. In a comparative study of nurseries using conventional plant breeding techniques and plant tissue culture, it was observed that production processes had undergone several changes as a result of the introduction of tissue culture. In traditional nurseries the production process was organized according to the simple division of labour. In contrast, plant tissue culture technology was introduced within a complex organizational structure with a formal hierarchy similar to that of the manufacturing industry. Plant tissue culture has ushered in the industrialization of horticulture.

scholarly journals Plant Tissue Culture- A New Tool for Vegetable Improvement (Indian scenario): A Review

2021 ◽  
Author(s):  
Priyanka Bijalwan ◽  
Shilpa .
Keyword(s):  
Tissue Culture ◽  
Embryo Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Plant Cells ◽  
Meristem Culture ◽  
Culture Techniques ◽  
Short Period ◽  
Plant Sciences

In vitro culture of plant cells/tissues is now routine using a range of explant types from many of the important vegetable and fruit crops. Successful technologies include isolation, culture of tissues, cells, protoplasts, organs, embryos, ovules, anthers and microspores and regeneration from them of complete plantlets. The development of plant tissue culture technology represents one of the most exciting advances in plant sciences. For example, the prospect of being able to introduce, develop, produce, transfer and conserve the existing gene pool of plant sciences by using tissue culture methods opens up new opportunities for researches and entrepreneurs. The term plant tissue culture should denote in vitro cultivation of plant cells or tissues in an unorganized mass, i.e., callus culture. Plant tissue culture techniques, in combination with recombinant DNA technology, are the essential requirements for the development of transgenic plants. However, culture techniques like anther/pollen/ovule culture, meristem culture can themselves be utilized for crop improvement or may serve as an aid to conventional breeding. In recent, isolated microspore culture has developed as a breeding tool and an experimental system for various genetic manipulations. The inherent potentiality of a plant cell to give rise to a whole plant, a capacity which is often retained even after a cell has undergone final differentiation in the plant body, is described as ‘cellular totipotency’. On the other hand, production of virus-free plants via meristem culture can reduce losses caused by phyto-pathogens. Embryo culture has many potential uses ranging from overcoming seed dormancy to facilitation of inter-specific hybridization. Protoplast fusion technique can be used for the transfer of cytoplasmic male sterility from one species to another in a short period of time. In cabbage, male sterile cybrids are being utilized by seed companies to produce hybrid seeds on commercial scale and at competitive rates. Plant tissue culture and cell culture are providing useful methods for germplasm storage either by low temperature storage of organized tissue, or cryopreservation of cell or embryo culture.

Alginate-based solid media for plant tissue culture

1989 ◽  
Vol 31 (1) ◽  
Author(s):  
KurtIngar Draget ◽  
Kjetill �stgaard ◽  
Olav Smidsr�d
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Solid Media
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