Fifty million years of Archean evolution is recorded in the Manitouwadge greenstone belt and the Wawa-Quetico boundary region, from ca. 2720 Ma volcanism and subvolcanic plutonism associated with massive sulphide deposits and hydrothermal alteration zones, to 2689-2687 Ma and 2680-2677 Ma synkinematic plutonism. In the greenstone belt, greywackes were deposited after 2693 Ma, post-dating local volcanism by at least 25 Ma, and requiring that the volcanic-sedimentary contact is an unconformity or a fault. In migmatitic greywackes in the Quetico subprovince, detrital zircons limit the depositional age to <2690 Ma, permitting correlation of greywackes across the Wawa-Quetico subprovince boundary. Upward-facing inclined F2 folds that deform the volcanic-sedimentary contact are bracketed by the 2687 ± 2 Ma Loken Lake pluton, which shows strong D2 fabrics, and by 2680+4-3 Ma foliated granite which cuts D2 fabrics. Dextral transpression producing regional F3 folds and the overall east-west trends of the Wawa-Quetico boundary region post-dated the 2680 ± 2 Ma Nama Creek pluton. Field relationships and isotopic ages support correlation of greywackes across the subprovince boundary, and demonstrate that most or all of the ductile deformation post-dated sedimentation. Ductile structures, especially those associated with dextral transpression, are not directly related to juxtaposition of the Wawa and Quetico subprovinces, as these were already contiguous, either through sedimentation on a volcanic substrate or as a result of earlier cryptic structures. Our results imply that the belt-like configuration of the subprovinces, emphasized in accretionary models of the Superior Province, is a relatively late feature that overprints older, tectonically significant structures.