Gingival crevicular fluid and serum levels of resistin in obese and non-obese subjects with and without periodontitis and association with single nucleotide polymorphism at −420

The relationship between nitric oxide production and metabolic disorders and the role of endothelial nitric oxide synthase (eNOS or NOS3) in metabolic syndrome (MS) remain poorly understood and need deeper investigation. In this context the role of the NOS3 gene in pathogenesis of MS is of special interest. The aim of the study was to investigate association of NOS3 single nucleotide polymorphism C774T with risk of MS in the Slavic population of the Kaliningrad region and the relationship of this polymorphic variant with some parameters of endothelial dysfunction. The study included 128 patients (48 men and 80 women aged from 36 to 52 years) with MS. The control group consisted of 126 healthy volunteers (60 men and 66 women aged from 30 to 40 years). Genotyping was performed by real-time PCR. Serum nitrite levels were determined spectrophotometrically by the Griess method. Serum levels of endothelin-1 and eNOS were evaluated by ELISA. The study has shown association of T allele (OR=2.06; p=0.0004; CI: 1.38-3.08) and CT genotype (OR=1.97; p=0.014; CI: 1.14-3.40 ) C774T polymorphism of the NOS3 gene with risk of MS in the Slavic population of the Kaliningrad region. Allele C (OR=0.48; p=0.0004; CI: 0.32-0.72) and homozygous CC genotype (OR=0.41; p=0.001; CI: 0.24-0,69) C774T polymorphism of the NOS3 gene were associated with reduced risk of the development of MS. Significant differences in serum levels of eNOS and endothelin-1 depended on the CT and TT genotypes of C774T polymorphism of the NOS3 gene in MS.

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Correlation of C-reactive protein levels, gene polymorphism and platelets count in Dengue infection

Journal of the Pakistan Medical Association ◽

10.47391/jpma.326 ◽

2020 ◽

pp. 1-16

Author(s):

Sana Shamshad ◽

Sara Khan ◽

Ghazala Kaukab Raja ◽

Muhammad Sheeraz Ahmad ◽

Muhammad Javaid Asad ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Dengue Infection ◽

Serum Levels ◽

Amplification Refractory Mutation System ◽

Acid Extraction ◽

Nucleotide Polymorphism ◽

C Reactive Protein ◽

Single Nucleotide ◽

Protein Levels ◽

Reactive Protein

Abstract Objective: To determine the correlation of polymorphism in C-reactive protein gene with variation in serum levels in dengue patients. Methods: The cross-sectional study was conducted at Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan, from October 2017 to October 2018, and comprised blood samples from dengue patients which were used to measure the serum levels of C-reactive protein. Deoxyribonucleic acid extraction followed by tetra amplification-refractory mutation system polymerase chain reaction was done to analyse the genotype variation T>G for single nucleotide polymorphism rs199953854 using allele-specific primers. Correlation of serum C-reactive protein levels with the C-reactive protein polymorphism in dengue patients was explored. Data was analysed using SPSS 21. Results: Of the 200 patients, 108(54%) had very high C-reactive protein levels, 48(24%) had levels slightly higher than the upper limit, 14(7%) had low and 30(15%) had normal levels. Also, 162(81%) patients had low platelets count. Amplification of only T alleles was noted. Conclusion: C-reactive protein levels were found to be increased with suppressed platelets count in dengue patients. Single nucleotide polymorphism rs199953854 appeared to have no polymorphism. Key Words: C-reactive protein, Dengue infection, rs199953854, Continuous...

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Role of theDGATGene C79T Single-Nucleotide Polymorphism in French Obese Subjects

Obesity Research ◽

10.1038/oby.2003.160 ◽

2003 ◽

Vol 11 (10) ◽

pp. 1163-1167 ◽

Cited By ~ 11

Author(s):

Sylvie Kipfer Coudreau ◽

Patrick Tounian ◽

Geneviève Bonhomme ◽

Philippe Froguel ◽

Jean-Philippe Girardet ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Obese Subjects

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Single nucleotide polymorphism rs7908486 of the tcf7l2 gene is highly associated with obesity in the Iraqi population

Archives of Biological Sciences ◽

10.2298/abs201213056m ◽

2020 ◽

pp. 56-56

Author(s):

Amera Mohammed ◽

Tahreer Al-Thuwaini ◽

Mohammed Al-Shuhaib

Keyword(s):

Single Nucleotide Polymorphism ◽

Fasting Blood Glucose ◽

Single Strand Conformation Polymorphism ◽

Tcf7l2 Gene ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Banding Patterns ◽

Specific Pcr ◽

Obese Subjects ◽

Tight Association

This study was conducted to assess the possible association between polymorphisms of the transcription factor 7-like 2 (TCF7L2) gene and obese Iraqi adults. DNA was obtained from 158 obese subjects and 142 controls. Two specific PCR fragments were designed to incorporate two highly frequent single nucleotide polymorphism (SNP)s within TCF7L2, rs11196208 and rs7908486. Both amplified loci were genotyped by PCR-single-strand conformation polymorphism (SSCP) followed by sequencing. Logistic regression analysis was performed to detect the association between both genetic variants and obesity. Concerning rs11196208-based amplicons, PCR-SSCP genotyping showed homogeneous banding patterns for all investigated samples. In contrast with rs11196208, three SSCP banding patterns in the rs7908486-based amplicons, GG, AG and AA, were observed. Individuals with the AA genotype showed significantly higher (P<0.05) body mass index (BMI), waist circumference (WC), fasting blood glucose (FBG), and insulin values than those with either AG or GG genotypes. Association analysis revealed that individuals with the A allele exhibited a greater risk of obesity than individuals with the B allele. Data indicate that rs7908486 SNP exerted a tight association with obesity. The study suggests implementing TCF7L2 rs7908486-based genotyping in the early detection of obesity.

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IL9 Single Nucleotide Polymorphism and Serum Levels of Interleukin 9 in Children with Asthma

Pediatric Allergy Immunology and Pulmonology ◽

10.1089/ped.2015.0547 ◽

2016 ◽

Vol 29 (1) ◽

pp. 34-37

Author(s):

Sedigheh Bahrami Mahneh ◽

Masoud Movahedi ◽

Mohammad Ali Bahar ◽

Arezou Rezaei ◽

Maryam Sadr ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Serum Levels ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Children With Asthma ◽

Interleukin 9

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THE ROLE OF GENETIC VARIATION SINGLE NUCLEOTIDE POLYMORPHISM T45G AND SINGLE NUCLEOTIDE POLYMORPHISM G276T OF THE ADIPONECTIN GENE IN FATTY LIVER PATHOMECHANISM ON OBESE MALE SUBJECT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11s1.26615 ◽

2018 ◽

Vol 11 (13) ◽

pp. 229

Author(s):

Sulaeman A ◽

Wahid S ◽

Sulaiman A ◽

Lawrence Sg

Keyword(s):

Insulin Resistance ◽

Single Nucleotide Polymorphism ◽

Genetic Variation ◽

Fatty Liver ◽

Adiponectin Gene ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Obese Subjects ◽

High Level ◽

Obese Male

Objective: The aim of this study was to observe the role of genetic variation T45G and G276T of adiponectin gene in pathomechanism of fatty liver on obese subjects.Methods: An observational study with case–control design was conducted on 94 obese male subjects (50 subjects are obese male with fatty liver and 44 subjects are obese male without fatty liver). The research is taken place in Prodia Clinical Laboratory Makassar to determine genetic variation single nucleotide polymorphism (SNP) T45G (Genotype TT, thyroglobulin [TG], and GG) and SNP G276T (Genotype GG, GT, and TT) of adiponectin gene, technique of polymerase chain reaction-restriction fragment length polymorphism was used. The level of adiponectin, soluble tumor necrosis factor-α receptor 2 (sTNF-αR2), and insulin serum were measured by enzyme-linked immunosorbent assay method, meanwhile, fatty liver was detected by ultrasonography.Results: The result of the study showed genetic variation, T45G of adiponectin gene was genotype TT 62.8 %, genotype TG 30.9%, and genotype GG 6.3%; meanwhile, genetic variation G276T of adiponectin gene was genotype GG 43.6%, genotype GT 38.3 %, and genotype TT 18.1 %. There was no significant correlation of genetic variation T45G as well as G276T of adiponectin gene, to the level of adiponectin serum (>0.05). Insulin resistance was more frequent on genotype TT genetic variation T45G of adiponectin gene as compare to that on TG+GG (p=0.069). Genotype TT on genetic variation T45G of adiponectin gene was significant correlated with fatty liver (p=0.010). Genotype TG+TT on genetic variation G276T of adiponectin gene was more likely to have insulin resistance and fatty liver than that of genotype GG. Allele T carrier on genetic variation T45G and G276T of adiponectin gene had a higher chance to have insulin resistance and fatty liver as compare to that of allele G carrier. The odds ratio of having fatty liver insulin resistance is 5.3, genotype TT on genetic variation T45G of adiponectin gene is 3.8, low level of adiponectin is 3.4, and high level of sTNF-αR2 is 3.3.Conclusion: Genotype TT on genetic variation T45G of adiponectin gene has a role in fatty liver on obese subjects. Genotype TG+TT on genetic variation G276T of adiponectin gene was more high frequent to have fatty liver compare to that on genotype GG. Allele T carrier on genetic variation T45G of adiponectin gene had higher frequency on the occurrence of insulin resistance than that on allele G carrier. Insulin resistance has the highest influence as compare to genotype TT on genetic variation T45G of adiponectin gene, low level of adiponectin serum, high level of sTNF-αR2 serum in the pathomechanism of fatty liver in obese subjects.

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