scholarly journals Identification and Antifungal susceptibility testing of Candida species: A Comparison of Vitek-2 system with conventional and molecular methods

2016 ◽  
Vol 8 (4) ◽  
pp. 139 ◽  
Author(s):  
Ravinder Kaur ◽  
MeghSingh Dhakad ◽  
Ritu Goyal ◽  
Absarul Haque ◽  
Gauranga Mukhopadhyay
Keyword(s):  
Candida Species ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Molecular Methods ◽  
Vitek 2

scholarly journals Frequency of candida infection among intensive care unit patients and their susceptibility profile

2021 ◽  
Vol 7 (3) ◽  
pp. 133-136
Author(s):  
Umar Farooq ◽  
Ayushi Jain ◽  
Sudhir Singh ◽  
Vasundhara Sharma ◽  
Shweta R Sharma ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Central Line ◽  
Antifungal Susceptibility Testing ◽  
Clinical Samples ◽  
Human Beings ◽  
High Susceptibility ◽  
Vitek 2 ◽  
Isolated Species

species are responsible for causing many health care associated and central line associated infections. They are responsible for causing opportunistic infection in human beings. Genus of is composed of a heterogeneous group of organsims.Invasive infections of mainly caused by , , & . The main objective of this study was to isolates and Non- albicans and their antifungal susceptibility testing.Thestudy was carried out in the Department of Microbiology, in Tmu Hospital Moradabad. Total numbers of 806 clinical samples were processed in which 206 isolates were taken for . Isolation and antifungal susceptibility testing done by Vitek-2 system.Out of 206 samples 77(37%) were and 129(63%) were Non-albicans (NAC). Maximum isolated species were 77(37%), followed by 70(34%), 24(12%), C. glabrata 19(9%), 12(6%), C. krusei 3(1%), C.african 1(1%).Infection caused by NAC species have increased. was the most common isolated species. ,and were shown high susceptibility to fluconazole and voriconazole. Amphotericin B, Caspofungin, Micafungin and Flucytosine shows high susceptibility towards other candida species.

scholarly journals Identification of Fungal Pathogens in Otomycosis and Their Drug Sensitivity: Our Experience

2018 ◽  
Vol 22 (04) ◽  
pp. 400-403 ◽  
Author(s):  
Khaled Ali ◽  
Mahmood Hamed ◽  
Hameda Hassan ◽  
Amira Esmail ◽  
Abeer Sheneef
Keyword(s):  
Enzymatic Activity ◽  
Candida Species ◽  
Fungal Pathogens ◽  
Antifungal Agents ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Antifungal Drugs ◽  
High Recurrence Rate ◽  
Fungal Culture

Introduction Otomycosis is a common problem in otolaryngology practice. However, we usually encounter some difficulties in its treatment because many patients show resistance to antifungal agents, and present high recurrence rate. Objectives To determine the fungal pathogens that cause otomycosis as well as their susceptibility to the commonly used antifungal agents. Additionally, to discover the main reasons for antifungal resistance. Methods We conducted an experimental descriptive study on 122 patients clinically diagnosed with otomycosis from April 2016 to April 2017. Aural discharge specimens were collected for direct microscopic examination and fungal culture. In vitro antifungal susceptibility testing was performed against the commonly used antifungal drugs. We tested the isolated fungi for their enzymatic activity. Results Positive fungal infection was found in 102 samples. The most common fungal pathogens were Aspergillus and Candida species, with Aspergillus niger being the predominant isolate (51%). The antifungal susceptibility testing showed that mold isolates had the highest sensitivity to voriconazole (93.48%), while the highest resistance was to fluconazole (100%). For yeast, the highest sensitivity was to nystatin (88.24%), followed by amphotericin B (82.35%), and the highest resistance was to terbinafine (100%), followed by Itraconazole (94.12%). Filamentous fungi expressed a high enzymatic ability, making them more virulent. Conclusion The Aspergillus and Candida species are the most common fungal isolates in otomycosis. Voriconazole and Nystatin are the medications of choice for the treatment of otomycosis in our community. The high virulence of fungal pathogens is owed to their high enzymatic activity. Empirical use of antifungals should be discouraged.

scholarly journals Disk diffusion Method in Enriched Mueller Hinton agar for determining susceptibility of Candida isolates from various clinical specimens

2020 ◽  
Vol 28 (1) ◽  
pp. 28-33
Author(s):  
Nabeela Mahboob ◽  
Hasina Iqbal ◽  
Mushtaque Ahmed ◽  
Md Mehedi Hasan Magnet ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Methylene Blue ◽  
Amphotericin B ◽  
Candida Species ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Disk Diffusion ◽  
Clinical Specimens ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Background: Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases. Recently there is a serious concern with increased resistance of antifungal drugs and its consequences. Thus, identification of Candida and its antifungal susceptibility testing has a paramount significance in the management of Candidal infections. The aim of the study was to determine antifungal susceptibility pattern of Candida by Mueller-Hinton agar media supplemented with glucose and methylene blue for disk diffusion testing of fluconazole, miconazole, clotrimazole, amphotericin B and nystatin. Methods: A total of 35 Candida species was isolated from 2000 clinical specimens over 6 month’s period from July 2016 to December 2016. Growths on Blood agar and chromogenic agar were evaluated for colony appearance and microscopic examination. Antifungal susceptibility testing was performed by disk diffusion using Mueller-Hinton agar supplemented with glucose and methylene blue. Results: Candida species were more sensitive to clotrimazole (88.58%) and amphotericin B (88.58%) followed by nystatin ((77.14%), miconazole (74.29%) whereas fluconazole showed the highest level of resistance (60%). Conclusions: The increase in resistance to fluconazole is of serious concern as it is the most commonly used azole for candidiasis. The sensitivity profile of Candida isolates will be helpful to choose appropriate antifungal agents, thus decreasing patient’s morbidity and mortality. J Dhaka Medical College, Vol. 28, No.1, April, 2019, Page 28-33

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