scholarly journals Spectrophotometric and Reversed-Phase High-Performance Liquid Chromatographic Method for the Determination of Doxophylline in Pharmaceutical Formulations

2010 ◽  
Vol 2 (3) ◽  
pp. 289-296 ◽  
Author(s):  
A.H. Patel ◽  
A.D. Captain ◽  
H.R. Joshi
Keyword(s):  
High Performance ◽  
Chromatographic Method ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

scholarly journals A Reversed-Phase High-Performance Liquid Chromatographic Method for the Determination of Zafirlukast in Pharmaceutical Formulations and Human Plasma

2006 ◽  
Vol 89 (6) ◽  
pp. 1557-1564 ◽  
Author(s):  
İncilay SÜsÜl ◽  
Sacide AltinÖz
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Chromatographic Method ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Spiked Plasma ◽  
Liquid Chromatographic

Abstract Zafirlukast (ZAF) is a leukotriene receptor antagonist used in the treatment of chronic asthma. In this study, a simple and sensitive reversed-phase, high-performance liquid chromatographic method was developed for the determination of ZAF in pharmaceutical formulations and human plasma. Piribedil was used as an internal standard. Analysis was carried out on a Nucleosil C18 100 Å(150 × mm 4.6 mm id, 5 ωm) column with acetonitrilepH 3.0 acetate buffer (70 30, v/v) as the mobile phase at a flow rate of 0.8 mL/min. The peak was detected by an ultraviolet detector set at a wavelength of 240 nm. The retention times were about 3.9 min for piribedil and 5.8 min for ZAF. The developed method was applied to the determination of ZAF in its pharmaceutical formulation and spiked human plasma. For quantification of ZAF in spiked plasma, proteins were precipitated with ethanol before chromatographic analysis. The calibration range was linear from 49.69437.50 ng/mL in spiked plasma. The absolute recovery from spiked plasma was 98.73 ± 0.42% at a concentration of 254.78 ng/mL of ZAF. No endogenous substances from plasma were found to interfere.

scholarly journals An Isocratic Reversed-Phase High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Benzoyl Peroxide and the Related Compounds Benzoic Acid, Benzaldehyde, Ethyl Benzoate, Methylparaben, and Propylparaben in Dermal Preparations

2008 ◽  
Vol 91 (5) ◽  
pp. 1025-1036 ◽  
Author(s):  
Mohammed Shahid Ali ◽  
Syed Rafiuddin ◽  
Muhammad Imran Munir ◽  
Mohsin Ghori ◽  
Aamer Roshanali Khatri
Keyword(s):  
Benzoic Acid ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Ethyl Benzoate ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Related Compounds

Abstract A reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of benzoyl peroxide and the related compounds benzoic acid (BA), methylparaben, benzaldehyde, propylparaben, and ethyl benzoate. The compounds are separated on a column containing octadecyl silane chemically bonded to porous silica particles. The mobile phase is acetonitrilebuffer (45 + 55, v/v). Solutions are injected into the chromatographic system under isocratic conditions at a constant flow rate of 1.5 mL/min with UV detection at 235 nm. Analysis of stability samples showed rapid accumulation of BA by thermal degradation. A rationale has been established for the acceptable limit of BA in the formulation, which already contains BA (0.2) as a preservative. The proposed method is efficient and determines the active compound and 5 related compounds in a run time of 20 min. The method was validated according to the guidelines of the International Conference on Harmonization and demonstrated good agreement with the validation requirements.

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