scholarly journals Clinical efficacy of a combination of Percoll continuous density gradient and swim-up techniques for semen processing in HIV-1 serodiscordant couples

2017 ◽  
Vol 19 (2) ◽  
pp. 208 ◽  
Author(s):  
Naoaki Kuji ◽  
Osamu Inoue ◽  
Hiroe Ito ◽  
Mitsutoshi Yamada ◽  
Toshio Hamatani ◽  
...  
Keyword(s):  
Density Gradient ◽  
Clinical Efficacy ◽  
Serodiscordant Couples ◽  
Continuous Density ◽  
Semen Processing ◽  
Hiv 1

Buoyant density and sedimentation dynamics of HIV-1 in two density-gradient media for semen processing

2008 ◽  
Vol 90 (5) ◽  
pp. 1983-1987 ◽  
Author(s):  
Naoaki Kuji ◽  
Tsuyoshi Yoshii ◽  
Toshio Hamatani ◽  
Hideji Hanabusa ◽  
Yasunori Yoshimura ◽  
...  
Keyword(s):  
Density Gradient ◽  
Buoyant Density ◽  
Sedimentation Dynamics ◽  
Semen Processing ◽  
Hiv 1

scholarly journals Comparison of Tissue Loading Before and After the Creation of a Continuous Density Gradient in Porcine Islet Purification

Cell Medicine ◽  
2018 ◽  
Vol 10 ◽  
pp. 215517901878134 ◽  
Author(s):  
Chika Miyagi-Shiohira ◽  
Yoshiki Nakashima ◽  
Nana Ebi ◽  
Eri Hamada ◽  
Yoshihito Tamaki ◽  
...  
Keyword(s):  
Density Gradient ◽  
Stimulation Index ◽  
High Yield ◽  
Pancreatic Islet Transplantation ◽  
Post Transplantation ◽  
Porcine Pancreas ◽  
Purification Method ◽  
Continuous Density ◽  
Porcine Islet ◽  
The Impact

The purification step is one of the most important and difficult procedures in islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from the porcine pancreas. In this study, we evaluated the impact of the timing of tissue loading on porcine islet purification using large plastic bottles. One method involved loading digested tissue after creating a continuous density gradient (tissue after gradient [TAG]). The other method involved loading digested tissue before creating a continuous density gradient (tissue before gradient [TBG]). There were no significant differences between TAG and TBG in terms of the islet yield, rates of viability and purity, score, and in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of post-transplantation normoglycemia. Our study shows the equivalency of these two methods of islet purification.

Influence de la cytochalasine A sur la composition de fractions subcellulaires d'hyphes en croissance du Mucor mucedo L. I. Composition du plasmalemme

1988 ◽  
Vol 34 (11) ◽  
pp. 1256-1265 ◽  
Author(s):  
Abdou Ahmed Abd El Razak El Mougith ◽  
Jean-Louis Fonvieille ◽  
Robert Dargent ◽  
Jacques Rami ◽  
Jane-Marie Touzé-Soulet
Keyword(s):  
Protein Content ◽  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Sucrose Density Gradient ◽  
Point Of View ◽  
Continuous Density ◽  
Mucor Mucedo ◽  
Phospholipid Ratio ◽  
Enzymatic Markers

The plasma membrane of young hyphae of Mucor mucedo L. growing in presence or absence of cytochalasine A was isolated by continuous density gradient centrifugation using Percoll at 10% or on discontinuous sucrose density gradient. Isolated membranes were characterized by enzymatic markers and cytochemical reactions, using electron microscopy. Lipid composition and protein content were determined. From the enzymatic point of view, the cytochalasine A induced a decrease (60%) in ATPase activity and with regard to the chemical composition of the membrane, a decrease in sterol content and in the sterol – phospholipid ratio as well as a decrease in protein content and an increase in the proportion of cysteine relative to other amino acids.

Safety and efficacy of sperm washing in HIV-1-serodiscordant couples where the male is infected: results from the European CREAThE network

AIDS ◽  
2007 ◽  
Vol 21 (14) ◽  
pp. 1909-1914 ◽  
Author(s):  
Louis Bujan ◽  
Lital Hollander ◽  
Mathieu Coudert ◽  
Carole Gilling-Smith ◽  
Alexandra Vucetich ◽  
...  
Keyword(s):  
Serodiscordant Couples ◽  
Safety And Efficacy ◽  
Sperm Washing ◽  
Hiv 1

scholarly journals Safety of sperm washing and ART outcome in 741 HIV-1-serodiscordant couples

2006 ◽  
Vol 22 (3) ◽  
pp. 772-777 ◽  
Author(s):  
V. Savasi ◽  
E. Ferrazzi ◽  
C. Lanzani ◽  
M. Oneta ◽  
B. Parrilla ◽  
...  
Keyword(s):  
Serodiscordant Couples ◽  
Art Outcome ◽  
Sperm Washing ◽  
Hiv 1

scholarly journals HLA Class I and Plasma Viral Load of HIV-1 in Sexually Transmitted and Reproductive Tract Infections among Heterosexual Serodiscordant couples in Nigeria

2021 ◽  
Author(s):  
NM Otuonye ◽  
Luo Ma ◽  
Chris Chinweokwu ◽  
MN Aniedobe ◽  
RN Okoye ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Viral Load ◽  
Plasma Viral Load ◽  
Reproductive Tract ◽  
Hla Class I ◽  
Class I ◽  
Serodiscordant Couples ◽  
E Coli ◽  
Tract Infections ◽  
Hiv 1

ABSTRACTBackgroundThis study investigated HLA Class I in Long Term Non-progressors (LTNPs) and plasma viral load in Sexually Transmitted and Reproductive Tract Infections (STIs/RTIs) associated with Heterosexual HIV-1 transmission among serodiscordant couples in Nigeria.MethodsA total of 271 serodiscordant and concordant couples (HIV positive and negative) were enrolled, blood samples were collected from the subjects by venipuncture. HLA class I (with specific primers), plasma viral load, CD4+ analysis was done. Endocervical/urethral swabs and early morning urine samples were collected by standard microbiological methods. These were screened by microscopy, culture, antibiogram, and biochemical tests with a view to identify aetiologic agents of co-infections with HIV.ResultsThe Participants age ranged from ≥ 21- < 50years. The index whose plasma viral loads were 10,001-100,000 copies/ml had STIs/RTIs 32(60.9% p=0.059). Staphylococcus aureus and Escherichia coli (22.1%) were isolated from the index (HIV positive subject) while 14.5% of Staphylococcus aureus and 27.2% of E coli were isolated from their partners (HIV negative subject). Staphylococcus aureus and E coli are normal flora but because the patients are Immunocompromised as a result of positivity to HIV, Staphylococcus aureus and E. coli in this context becomes opportunistic thereby, causing genital tract infections. Staphylococcus from the index showed more sensitivity to Amoxicillin/clavulanate (95.4%/90.4%) compared to the partners (55.1%/73.5%) and more resistant to Ceftazidime (81.4%) compared to the partners (68.9%). LTNPs were 28(8.51%) among the index. HLA-B alleles: B*5701 (9.2%), B*5703 (4.6%) and B*5801(12.5%) were identified for viral control at late stage of HIV infection while A*1 (4.6%), and C*0701 (29.1%) were protective alleles observed. HLA-B*0702 (33.3%), B*4201/A*2301(4.6%) respectively were susceptible alleles associated with seroconversion among LTNPs.ConclusionThe microorganisms isolated from the index were associated with high viral loads and are independent makers to HIV-1 transmission among serodiscordant couples. Individuals associated with HLA class I alleles identified among LTNPs were those significantly associated with resistance and susceptible to HIV-1 infections.

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