scholarly journals The use of traditional and novel techniques to determine the hardness and indentation properties of immature radicular dentin treated with antibiotic medicaments followed by ethylenediaminetetraacetic acid

2014 ◽  
Vol 08 (04) ◽  
pp. 521-527 ◽  
Author(s):  
Ghaeth H. Yassen ◽  
Sarah S. Al-Angari ◽  
Jeffrey A. Platt
Keyword(s):  
Reference Point ◽  
Root Canal ◽  
Vickers Microhardness ◽  
Ethylenediaminetetraacetic Acid ◽  
Triple Antibiotic Paste ◽  
Canal Surfaces ◽  
Root Canal Dentin ◽  
Intact Root ◽  
Double Antibiotic Paste ◽  
One Way Anova

ABSTRACT Objective: The objective was to investigate the effect of intracanal antibiotic medicaments followed by ethylenediaminetetraacetic acid (EDTA) on the indentation properties and hardness of radicular dentin using a BioDent reference point indenter and a traditional microhardness technique, respectively. Materials and Methods: Specimens with intact root canal dentin surfaces and polished radicular dentin specimens were obtained from immature human premolars. Each type of specimen was randomly assigned (n = 10 per group) and treated with either double antibiotic paste (DAP) for 4-week followed by EDTA for 5 min, triple antibiotic paste (TAP) for 4-week followed by EDTA for 5 min, EDTA for 5 min or Hank's balanced salt solution (control). The BioDent reference point indentor and Vickers microhardness tester were used to measure the indentation properties of root canal surfaces and the hardness of polished dentin specimens, respectively. One-way ANOVA followed by Fisher's protected least significant differences were used for statistical analyses. Results: Both types of radicular dentin treated with antibiotic pastes and/or EDTA had a significant increase in the majority of indentation properties and a significant reduction in hardness compared to the untreated dentin. Furthermore, treatment of dentin with antibiotic pastes and EDTA caused significant increases in indentation properties and a significant reduction in hardness compared to EDTA-treated dentin. However, the RPI technique was not able to significantly differentiate between DAP + EDTA and TAP + EDTA-treated dentin. Conclusion: Dentin treated with antibiotic medicaments followed by EDTA had a significant increase the indentation properties and significantly reduction in hardness of radicular dentin.

scholarly journals Erratum: Number of Dental Stem Cells on Root Canal Dentin after Application of Triple Antibiotic Paste or Calcium Hydroxide: An In Vitro Study

2019 ◽  
Vol 13 (02) ◽  
pp. e1-e1
Author(s):  
Konstantinos Gougousis ◽  
Dimitrios G. Giannakoulas ◽  
Vasiliki Taraslia ◽  
Anastasia Agrafioti ◽  
Ema Anastasiadou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Dental Stem Cells ◽  
Triple Antibiotic Paste ◽  
Root Canal Dentin

scholarly journals Effect of Different Irrigation Solutions on the Diffusion of MTA Cement into the Root Canal Dentin

Materials ◽  
2020 ◽  
Vol 13 (23) ◽  
pp. 5472
Author(s):  
José Pedro Martinho ◽  
Sara França ◽  
Siri Paulo ◽  
Anabela Baptista Paula ◽  
Ana Sofia Coelho ◽  
...  
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Treatment Success ◽  
Confocal Laser ◽  
Control Group ◽  
Middle Section ◽  
Group 2 ◽  
Root Canal Dentin ◽  
Group 1

(1) Aim: This study aims to analyze the in vitro infiltration of a silicate root canal sealer into dentinal tubules after using different endodontic irrigating solutions. (2) Methods: Twenty-nine teeth with single roots were separated into three groups according to the final irrigation protocol: G1 n = 10) = 17% EDTA (ethylenediaminetetraacetic acid) + 3.0% sodium hypochlorite (NaOCl), G2 (n = 10) = 17% EDTA + 2.0% chlorhexidine and G3 (Control group, n = 9) = 17% EDTA + saline solution. Root canals were filled using cold lateral compaction technique with MTA Fillapex sealer and gutta-percha. The sealer was labeled with rhodamine B. The teeth were segmented at the middle and third apical sections, which were visualized using 10× confocal laser microscopy to determine the sealer penetration percentage. (3) Results: In the apical section, no statistically significant differences were found between the groups regarding sealer penetration. In the middle section, Group 1 obtained the highest percentage, and Group 2 the lowest (p = 0.004). Group 1 also presented statistically significant differences in the Control Group (p = 0.031) and had close sealer penetration values. Meanwhile, the Control Group (p = 0.023) and Group 2 (p = 0.029) revealed a significant decrease of sealer penetration between the apical and middle sections. (4) Conclusion: The obtained results support that final irrigation with NaOCl promoted similar sealer penetration in the apical and middle sections. On the other hand, a significant decrease in the sealer penetration of the middle section was observed for the chlorhexidine and saline groups. Compared to other irrigant solutions, NaOCl promotes more uniform sealer penetration, which can correlate with better sealing and, consequently, higher endodontic treatment success.

Decalcifying Effect of Different Ethylenediaminetetraacetic Acid Irrigating Solutions and Tetraclean on Root Canal Dentin

2012 ◽  
Vol 38 (9) ◽  
pp. 1239-1243 ◽  
Author(s):  
Claudio Poggio ◽  
Alberto Dagna ◽  
Marco Colombo ◽  
Federico Rizzardi ◽  
Marco Chiesa ◽  
...  
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Root Canal Dentin

scholarly journals Calcium loss on root canal dentin after Ethylenediaminetetraacetic acid (EDTA) application with different varians

2019 ◽  
Vol 4 (2) ◽  
pp. 79
Author(s):  
Juni J. Nugroho ◽  
Christine A. Rovani ◽  
Hermiati Daharuddin ◽  
Aries C. Trilaksana ◽  
Nurhayaty Natsir ◽  
...  
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Root Canal Dentin ◽  
Calcium Loss

scholarly journals Investigation of the effects of different chelating solutions on the microhardness and surface roughness of root canal dentin

2019 ◽  
Vol 9 (1) ◽  
pp. 22-29
Author(s):  
Celalettin Topbaş ◽  
Özkan Adıgüzel ◽  
Özlem Çölgeçen
Keyword(s):  
Surface Roughness ◽  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Micro Hardness ◽  
Dentin Surface ◽  
Significant Difference ◽  
Diamond Saw ◽  
The Difference ◽  
Root Canal Dentin ◽  
Roughness Measurements

Introduction: The purpose of this study is to examine the effect of different chelating solutions on microhardness and surface roughness of root canal dentin. Methodology: The crowns of sixty recently extracted maxillary central incisors were separated by diamond burs from the cemento-enamel junction. Roots are divided longitudinally into two pieces with diamond saw. The obtained samples were embedded in autopolymeric-acrylic and the dentin-surfaces of the teeth were grounded. Micro-hardness and surface-roughness measurements were carried out before the chelating processes. First group, the specimens were treated with gel-formed 17% Ethylenediaminetetraacetic acid [EDTA] (JE), second group, specimens were treated with 17% Liquid-formed EDTA (LE), third group, specimens were treated with 20% Citric acid (CA), and the last group specimens were treated with 7% Maleic acid (MA) for 120 seconds. After application of the chelating agents, micro-hardness and surface-roughness measurements of all samples were performed again. The difference between the initial and final measurements was calculated and statistically analyzed. One Way Analysis of Variance ANOVA was used for statistical-analysis and statistically significant difference was observed between the groups (p <0.05). Results: According to the test results, the microhardness of LE was statistically equal to that of JE and caused the least decrease in micro-hardness, SA decreased micro-hardness more than LE and JE, and MA decreased dentin micro-hardness more than all other solutions. And surface-roughness results are: MA increased surface-roughness more than other groups, SA and JE less roughened the dentin surface than MA, and there was no significant difference between the two solutions, LE caused less increase in dentin surface roughness compared to all solutions. Conclusions: Besides the use of EDTA and SA, the clinical use of MA can be considered, and it should also be noted that EDTA differs in terms of surface roughness between liquid and gel forms.   How to cite this article: Topbaş C, Adıgüzel Ö, Çölgeçen Ö. Investigation of the effects of different chelating solutions on the microhardness and surface roughness of root canal dentin. Int Dent Res 2019;9(1):22-9.   Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

Effects of QMix and ethylenediaminetetraacetic acid on decalcification and erosion of root canal dentin

2016 ◽  
Vol 79 (11) ◽  
pp. 1056-1061 ◽  
Author(s):  
Hacer Aksel ◽  
Ahmet Serper ◽  
Sukru Kalayci ◽  
Guler Somer ◽  
Cevat Erisken
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Root Canal Dentin

Chemical and Ultramorphologic Effects of Ethylenediaminetetraacetic Acid and Sodium Hypochlorite in Young and Old Root Canal Dentin

2012 ◽  
Vol 38 (2) ◽  
pp. 204-208 ◽  
Author(s):  
H. Ozgur Ozdemir ◽  
Hatice Dogan Buzoglu ◽  
Semra Çalt ◽  
Zafer C. Çehreli ◽  
Elif Varol ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Root Canal Dentin

scholarly journals Number of Dental Stem Cells on Root Canal Dentin after Application of Triple Antibiotic Paste or Calcium Hydroxide: An In Vitro Study

2019 ◽  
Vol 13 (02) ◽  
pp. 161-165
Author(s):  
Konstantinos Gougousis ◽  
Dimitrios G. Giannakoulas ◽  
Vasiliki Taraslia ◽  
Anastasia Agrafioti ◽  
Ema Anastasiadou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Statistical Tests ◽  
Deciduous Teeth ◽  
Test Material ◽  
Root Canals ◽  
Triple Antibiotic Paste ◽  
Canal Surfaces ◽  
Group A

Abstract Objective The aim of this study was to evaluate the viability of stem cells from exfoliated and deciduous teeth (SHED) on dentin surface treated with triple antibiotic paste or calcium hydroxide. Materials and Methods Nine single-rooted extracted premolars were prepared appropriately and divided into three groups. In group A, the root canals were left empty, a triple antibiotic paste was placed in the root canals of group B, and calcium hydroxide was placed in the root canals of group C. After 1 week, the intracanal medicaments were removed, and stem cells were seeded on the treated surface of the specimens for 1 more week. The cells were stained and then observed under confocal microscope over the entire surface of each test material. Counting of the cells was made by Image J (3D) software, as well as manually. Statistical Analysis To investigate any statistically significant differences between the experimental groups, statistical tests including Kruskal–Wallis and Mann–Whitney U-test were performed. Significance level was set to P < 0.05, and all analyses were performed with SPSS IBM program, v. 21. Results Groups B and C showed statistically significantly higher number of cells compared to Group A, whereas cells developed in a substrate of calcium hydroxide residues appeared in majority with distinct cores and widened unlike other groups. Conclusions The effect of calcium hydroxide manifested better results regarding the number of stems cells on root canal surfaces.

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