Novel material for root-end applications for bio-allied sciences

Treating a patient with tooth pain is a multi-factorial process with many factors playing their role to warrant the success of the treatment procedure. One such important factor is to secure the root apex which is important for ensuring good prognosis in endodontics. Also in challenging situations like sealing a fractured root, resorbed apex, or an incompletely formed apex, the need of an intact root apex is much anticipated. Over the years, an array of novel products has been produced for root-end applications to achieve such a favorable condition. The objective of this review is to list and summarize such root-end materials. However, their merits, and claims of superiority over MTA, have to be ascertained via sustained research.

CERVICAL PULPOTOMY IN MATURE PERMANENT TOOTH WITH CARIOUS EXPOSURE: 3-YEAR-FOLLOW UP CASE REPORT

Objective. The objective of this case study is to manage irreversible pulpitis in a mature permanent molar with a conservative and cost-effective therapy, which is cervical pulpotomy. Summary. A cervical pulpotomy was performed on the second permanent mandibular molar with an irreversible pulpitis in a 30-year-old female patient.Taking the age of the patient and the condition of the underlying pulp tissue into account, cervical pulpotomy was planned to preserve the vitality of the intact root pulps using MTA. Follow-up examinations at 1 month, 3 months, 6 months, 1 year and 3 years revealed positive clinical and radiological results. The high success rate reported in this clinical case also in all systematic reviews for pulpotomy suggests that this procedure can offer an alternative to root canal treatment in teeth with irreversible pulpitis.

Root xylem in three woody angiosperm species is not more vulnerable to embolism than stem xylem

AimsSince plants are compartmentalised organisms, failure of their hydraulic transport system could differ between organs. We test here whether xylem tissue of stems and roots differ in their drought-induced embolism resistance, and whether intact roots are equally resistant to embolism than root segments.MethodsEmbolism resistance of stem and root xylem was measured based on the pneumatic technique for Acer campestre, A. pseudoplatanus and Corylus avellana, comparing also intact roots and root segments of C. avellana. Moreover, we compared anatomical features such as interconduit pit membrane between roots and stems.ResultsWe found a higher embolism resistance for roots than stems, although a significant difference was only found for A. pseudoplatanus. Interconduit pit membrane thickness was similar for both organs of the two Acer species, but pit membranes were thicker in roots than stems of C. avellana. Also, embolism resistance of an intact root network was similar to thick root segments for C. avellana.ConclusionOur observations show that root xylem is not more vulnerable to embolism than stem xylem, although more species need to be studied to test if this finding can be generalised. We also demonstrated that the pneumatic method can be applied to non-terminal plant samples.

Hemisection: Two case reports

Aim: This report discusses root and endodontic treatment as well as dental endodontic treatment of remaining roots using hemisection. An array of prosthetic approaches is described. Methodology: These two case reports describe multidisciplinary prosthetic restoration of teeth using a hemisection procedure. Results: It was possible to obtain successful results in problematic cases where the root failed to respond to endodontic treatment using a multidisciplinary approach combining endodontic treatment with restorative or prosthetic restorations of the intact root. Conclusions: Hemisection is a suitable alternative approach to extraction for the effective and conservative treatment of affected teeth using periodontal and endodontic treatment. How to cite this article: Falakaloglu S, Adiguzel O, Oztekin F, Deger Y, Ozdemir G. Hemisection: Two case reports Int Dent Res 2016;6:16-20. Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

Enhanced Mulberroside A Production from Cell Suspension and Root Cultures of Morus alba Using Elicitation

Morus alba L. has been used in Asian traditional medicine as an anti-inflammatory, anti-asthmatic, anthelmintic and as a whitening agent in cosmetic products. Mulberroside A is the major active compound from M. alba root bark. In this study, cell suspension and root cultures of M. alba were established, and the effect of the elicitors on the enhancement of mulberroside A production in M. alba was investigated. The cell suspension and root cultures of M. alba were exposed to elicitors and then mulberroside A contents were determined by an indirect competitive ELISA method. High levels of mulberroside A were obtained by addition of 100 and 200 μM salicylic acid with 24 h exposure time in cell suspension cultures (37.9 ± 1.5 and 34.0 ± 4.7 mg/g dry wt., respectively). Furthermore, addition of yeast extract at 2 mg/mL with 24 h exposure time can significantly increase mulberroside A contents from both cell suspension (3.2-fold) and root cultures (6.6-fold). Mulberroside A contents from both cell suspension and root cultures after treatment with elicitors are similar or higher than those found in the intact root and root bark of several years old M. alba. These results indicate that mulberry tissue cultures using the elicitation method are interesting alternative sources for mulberroside A production.

The use of traditional and novel techniques to determine the hardness and indentation properties of immature radicular dentin treated with antibiotic medicaments followed by ethylenediaminetetraacetic acid

ABSTRACT Objective: The objective was to investigate the effect of intracanal antibiotic medicaments followed by ethylenediaminetetraacetic acid (EDTA) on the indentation properties and hardness of radicular dentin using a BioDent reference point indenter and a traditional microhardness technique, respectively. Materials and Methods: Specimens with intact root canal dentin surfaces and polished radicular dentin specimens were obtained from immature human premolars. Each type of specimen was randomly assigned (n = 10 per group) and treated with either double antibiotic paste (DAP) for 4-week followed by EDTA for 5 min, triple antibiotic paste (TAP) for 4-week followed by EDTA for 5 min, EDTA for 5 min or Hank's balanced salt solution (control). The BioDent reference point indentor and Vickers microhardness tester were used to measure the indentation properties of root canal surfaces and the hardness of polished dentin specimens, respectively. One-way ANOVA followed by Fisher's protected least significant differences were used for statistical analyses. Results: Both types of radicular dentin treated with antibiotic pastes and/or EDTA had a significant increase in the majority of indentation properties and a significant reduction in hardness compared to the untreated dentin. Furthermore, treatment of dentin with antibiotic pastes and EDTA caused significant increases in indentation properties and a significant reduction in hardness compared to EDTA-treated dentin. However, the RPI technique was not able to significantly differentiate between DAP + EDTA and TAP + EDTA-treated dentin. Conclusion: Dentin treated with antibiotic medicaments followed by EDTA had a significant increase the indentation properties and significantly reduction in hardness of radicular dentin.

Regeneration of Canada Thistle (Cirsium arvense) from Intact Roots and Root Fragments at Different Soil Depths

In the present field study, the capability of Canada thistle to develop shoots from intact roots and root fragments at different soil depths was studied. The experiments were performed on four sites with high-density Canada thistle, with three or four replications per treatment. At each site, the soil in the plots was removed layer by layer (to 30 or 40 cm, depending on the site), within a 1 by 1-m quadrat, and spread out on a plastic sheet. All roots and other plant parts were removed, and the soil was either replaced without any root material (two sites), or the roots of the thistles were cut into 10-cm-long fragments and replaced into the source holes (two sites). The measured variables were shoot number and biomass. The number of shoots of Canada thistle decreased with increasing depth (P < 0.001) and increased with time. Additionally, the two factors interacted (P < 0.001) such that shoot development was slower from greater depths. Roots from ≤ 20 cm depth produced higher biomasses than did roots from below 20 cm depth. Replacement of root fragments did not affect the amount of biomass produced. It was concluded that the intact root system contributed considerably more to the total biomass produced by Canada thistle than did the root fragments in the upper soil layers.

Transpiration integrated model for root ion absorption under salinized condition

Salinization of crop fields is a pressing matter for sustainable agriculture under desertification and is largely attributed to root absorptive functions of the major crops such as maize. The rates of water and ion absorption of intact root system of maize plants were measured under the salinized condition, and the salt absorptive function of maize roots was analyzed by applying different two kinetic models of root ion absorption (i.e. the concentration dependent model and the transpiration integrated model). The absorption rates for salinization ions (Na+, Cl−, Ca2+ and Mg2+) were found to depend on ion mass flow through roots driven by the transpiration, and therefore the transpiration integrated model represented more accurately rates of root ion absorption. The root absorption of salinization ions was characterized quantitatively by two model parameters of Q′max and K′M involved in the transpiration integrated model, which are considered to relate to the potential absorbing power and the ion affinity of transport proteins on root cell membranes, respectively.