scholarly journals Characterization of human rotavirus subgroups and serotypes in children under five with acute gastroenteritis in a Saudi Hospital

2011 ◽  
Vol 18 (1) ◽  
pp. 22 ◽  
Author(s):  
ObeidE Obeid
Keyword(s):  
Acute Gastroenteritis ◽  
Children Under Five ◽  
Under Five ◽  
Human Rotavirus

Characterization of astrovirus-associated gastroenteritis in hospitalized children under five years of age

2017 ◽  
Vol 53 ◽  
pp. 94-99 ◽  
Author(s):  
Hassan Zaraket ◽  
Hadi Abou-El-Hassan ◽  
Khalil Kreidieh ◽  
Nadia Soudani ◽  
Zainab Ali ◽  
...  
Keyword(s):  
Hospitalized Children ◽  
Children Under Five ◽  
Under Five

scholarly journals Molecular Epidemiology and Clinical Risk Factors of Rotavirus Diarrhea: Single Center 5-Year Experience

2021 ◽  
Author(s):  
Ekin Soydan ◽  
İlknur Çağla ◽  
Mine Düzgol ◽  
Hurşit Apa ◽  
İlker Devrim ◽  
...  
Keyword(s):  
Acute Gastroenteritis ◽  
Laboratory Data ◽  
Clinical Findings ◽  
Study Data ◽  
Children Under Five ◽  
Under Five ◽  
Common Causes ◽  
Training And Research ◽  
Rotavirus Diarrhea

Objective: Acute gastroenteritis due to the rotavirus is one of the common causes of morbidity and mortality in children under five years of age. The objective of this study was to evaluate the epidemiological, clinical, and laboratory data of rotavirus diarrhea in hospitalized children under five years of age. Methods: All children between one month and 60 months old ages who were hospitalized in Health Sciences University Behçet Uz Child Disease and Pediatric Surgery Training and Research Hospital during September 2013 to August 2017 and diagnosed as acute gastroenteritis with rotavirus antigen test positive in feces were included in the current study. Data of the patients were collected retrospectively from medical records. Results: A total of 100 patients were evaluated.The median age of the patients was 13 months (IQR 5-45 months) and 54 patients were male and 46 were female.The highest hospitalization rate was in December, followed by November, and in October. The most common type of G9P (8) serotype was detected in rotavirus serotype analysis by PCR. Breastfeeding infants had milder clinic findings in comparison to the older ones. It was found that clinical findings were milder and Vesicari score was lower in infants who had breast milk. Vesikari score was found to be high in children with severe clinical findings. Conclusion: Rotavirus infection is important for all ages.In case of fever, increased numbers of vomiting and diarrhea, and higher Vesikari system scores may be associated with the severe clinical forms. Determination of rotavirus serotypes and clinical monitoring of genotypic changes are required.

scholarly journals Genetic Diversity and Epidemiology of Genogroup II Noroviruses in Children with Acute Sporadic Gastroenteritis in Shanghai, China, 2012-2017

2019 ◽  
Author(s):  
Lijuan Lu ◽  
Huaqing Zhong ◽  
Menghua Xu ◽  
Liyun Su ◽  
Lingfeng Cao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Analysis ◽  
Acute Gastroenteritis ◽  
Dominant Role ◽  
Phylogenetic Analyses ◽  
Age Groups ◽  
Detection Rates ◽  
Children Under Five ◽  
Under Five ◽  
Paediatric Age

Abstract Background Noroviruses (NoVs) are considered an important cause of acute gastroenteritis (AGE) across all age groups, especially in children under five years of age. We investigated the epidemiology of noroviruses in outpatient children from the Children’s Hospital of Fudan University in Shanghai, China. Methods Stool specimens were collected between January 2012 and December 2017 from 1433 children under five years of age with acute gastroenteritis. All samples were analysed by conventional reverse transcription-polymerase chain reaction (RT-PCR) for genogroup II NoVs amplifying both the RNA-dependent RNA polymerase (RdRp) and partial capsid genes. The Norovirus Genotyping Tool v.2.0 (https://www.rivm.nl/mpf/typingtool/norovirus/) was used for genotyping the strains, and phylogenetic analyses were conducted by MEGA 6.0. Results From 2012 to 2017, GII NoVs were detected in 15.4% (220/1433) of the samples, with the highest detection rate in children aged 7-12 months (19.2%, 143/746). The seasons with the highest prevalence of GII NoVs infection were autumn and winter . Based on genetic analysis of RdRp, GII.Pe (74.5%%, 137/184) was the most predominant RdRp genotype from 2013 to 2017, while GII.P4 played a dominant role in 2012 (55.6%, 21/36). Among the capsid genotypes, the most prevalent NoV genotype from 2012 to 2017 was GII.4 (73.6%, 162/220). On the basis of genetic analysis of RdRp and capsid sequences, the strains were clustered into 19 RdRp/capsid genotypes, and 12 of them were discordant, such as GII.Pe/GII.4-Sydney_2012, GII.P12/GII.3, GII.P7/GII.6, GII.Pe/GII.3, and GII.P16/GII.2. Starting with 2013, GII.Pe/GII.4-Sydney_2012 had completely replaced the pandemic GII.P4-2006b/GII.4-2006b subtype and was detected in children across all age groups. Conclusions The present study shows high detection rates and the genetic diversity of circulating NoV GII genotypes in paediatric AGE samples from Shanghai. The findings emphasize the importance of continuous molecular surveillance of emerging NoV strains.

scholarly journals Genetic Diversity and Epidemiology of Genogroup II Noroviruses in Children with Acute Sporadic Gastroenteritis in Shanghai, China, 2012-2017

2019 ◽  
Author(s):  
Lijuan Lu ◽  
Huaqing Zhong ◽  
Menghua Xu ◽  
Liyun Su ◽  
Lingfeng Cao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Analysis ◽  
Acute Gastroenteritis ◽  
Dominant Role ◽  
Phylogenetic Analyses ◽  
Age Groups ◽  
Detection Rates ◽  
Children Under Five ◽  
Under Five ◽  
Paediatric Age

Abstract Background Noroviruses (NoVs) are considered an important cause of acute gastroenteritis (AGE) across all age groups, especially in children under five years of age. We investigated the epidemiology of noroviruses in outpatient children from the Children’s Hospital of Fudan University in Shanghai, China. Methods Stool specimens were collected between January 2012 and December 2017 from 1433 children under five years of age with acute gastroenteritis. All samples were analysed by conventional reverse transcription-polymerase chain reaction (RT-PCR) for genogroup II NoVs amplifying both the RNA-dependent RNA polymerase (RdRp) and partial capsid genes. The Norovirus Genotyping Tool v.2.0 (https://www.rivm.nl/mpf/typingtool/norovirus/) was used for genotyping the strains, and phylogenetic analyses were conducted by MEGA 6.0. Results From 2012 to 2017, GII NoVs were detected in 15.4% (220/1433) of the samples, with the highest detection rate in children aged 7-12 months (19.2%, 143/746). The seasons with the highest prevalence of GII NoVs infection were autumn and winter . Based on genetic analysis of RdRp, GII.Pe (74.5%%, 137/184) was the most predominant RdRp genotype from 2013 to 2017, while GII.P4 played a dominant role in 2012 (55.6%, 21/36). Among the capsid genotypes, the most prevalent NoV genotype from 2012 to 2017 was GII.4 (73.6%, 162/220). On the basis of genetic analysis of RdRp and capsid sequences, the strains were clustered into 19 RdRp/capsid genotypes, and 12 of them were discordant, such as GII.Pe/GII.4-Sydney_2012, GII.P12/GII.3, GII.P7/GII.6, GII.Pe/GII.3, and GII.P16/GII.2. Starting with 2013, GII.Pe/GII.4-Sydney_2012 had completely replaced the pandemic GII.P4-2006b/GII.4-2006b subtype and was detected in children across all age groups. Conclusions The present study shows high detection rates and the genetic diversity of circulating NoV GII genotypes in paediatric AGE samples from Shanghai. The findings emphasize the importance of continuous molecular surveillance of emerging NoV strains. Keywords: Norovirus, RdRp/capsid genotypes, Epidemiology, Children, Acute gastroenteritis

scholarly journals Characterization of Haemophilus influenzae Obtained from Invasive Diseases in Cuban Children under Five Years of Age

1999 ◽  
Vol 94 (4) ◽  
pp. 477-478
Author(s):  
Isis Tamargo ◽  
Gilda Toraño ◽  
Oxandra Rodriguez ◽  
Miriam Perez ◽  
Alina Llop
Keyword(s):  
Haemophilus Influenzae ◽  
Children Under Five ◽  
Under Five ◽  
Invasive Diseases

scholarly journals PO 8524 MOLECULAR CHARACTERISATION OF THE NOROVIRUS STRAINS RESPONSIBLE FOR ACUTE DIARRHOEA IN CHILDREN UNDER FIVE YEARS OLD HOSPITALISED IN BRAZZAVILLE

2019 ◽  
Vol 4 (Suppl 3) ◽  
pp. A49.2-A49
Author(s):  
Vivaldie E Mikounou Louya ◽  
Félix Koukouikila-Koussounda ◽  
Christevy Vouvoungui ◽  
Simon Ch. Kobawila ◽  
Francine Ntoumi
Keyword(s):  
Acute Gastroenteritis ◽  
Acute Diarrhoea ◽  
Dual Infection ◽  
P Value ◽  
Published Data ◽  
Children Under Five ◽  
Under Five ◽  
Stool Samples ◽  
Triple Infection ◽  
The Difference

BackgroundAcute gastroenteritis is a leading cause of morbidity and mortality among children under five years old worldwide. Viruses are the most common responsible agent and norovirus is second after rotavirus. There is no published data on the occurrence of this agent in Brazzaville. This study aimed to determine the prevalence of norovirus infection and’to evaluate the risk factors in hospitalised children in Brazzaville, Congo.MethodsFrom June 2012 to June 2013, stool samples were collected from children under five years old hospitalised with acute gastroenteritis at Makelekele hospital. Rotavirus and adenovirus infections were already characterised in this population. A total of 545 samples were tested for GI and GII norovirus infections using nested duplex reverse-transcription–polymerase chain reaction with specific primers. The positive samples will be sequenced and analysed to determine the corresponding genotype.ResultsThe GI and GII norovirus infection were found in 148 samples (27, 14%) in this study. Males (28, 85%) were more infected than females (25%) but the difference was not significant. Norovirus infection was detected only in children under 24 months with a higher prevalence in the age group of 7–12 months (p value=0,048). The norovirus infection was detected throughout the year, but it peaked during the dry season (August-September). Dual infection of rotavirus and norovirus was detected in 65 cases (11, 9%), rotavirus-adenovirus in 8 cases (1, 5%), norovirus-adenovirus in 4 cases (0, 73%). Triple infection was detected in 3 cases (0, 55%).ConclusionThis study suggests that norovirus infection is the second cause of gastroenteritis after rotavirus in the study area. However, further surveillance investigations need to be pursued in other sentinel sites of the country.

scholarly journals Molecular characterization of norovirus infection responsible for acute diarrhea in Congolese hospitalized children under five years old in Brazzaville, Republic of Congo

2019 ◽  
Vol 88 ◽  
pp. 41-48 ◽  
Author(s):  
Vivaldie Mikounou Louya ◽  
Christevy Vouvoungui ◽  
Félix Koukouikila-Koussounda ◽  
Francisco Veas ◽  
Simon Charle Kobawila ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Acute Diarrhea ◽  
Hospitalized Children ◽  
Republic Of Congo ◽  
Children Under Five ◽  
Under Five

scholarly journals Genetic Diversity and Epidemiology of Norovirus in Children with acute sporadic gastroenteritis in Shanghai, China, 2012-2017

2019 ◽  
Author(s):  
Lijuan Lu ◽  
Huaqing Zhong ◽  
Menghua Xu ◽  
Liyun Su ◽  
Lingfeng Cao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Analysis ◽  
Acute Gastroenteritis ◽  
Dominant Role ◽  
Phylogenetic Analyses ◽  
Age Groups ◽  
Detection Rates ◽  
Children Under Five ◽  
Under Five ◽  
High Detection

Abstract Background Noroviruses are considered the important causes of acute gastroenteritis (AGE) across all age groups especially in children under five years. We investigated the prevalence and molecular epidemiology of norovirus in outpatient children from Children’s Hospital of Fudan University in Shanghai, China. Methods A total of 1433 stool specimens were collected from children under five years with acute gastroenteritis between January 2012 and December 2017. All the samples were analyzed by conventional reverse transcription-polymerase chain reaction (RT-PCR) for genogroup II targeting both the RNA-dependent RNA polymerase (RdRp) and partial capsid genes. Norovirus Genotyping Tool v.2.0 (https://www.rivm.nl/mpf/typingtool/norovirus/) was used for genotyping strains, and phylogenetic analyses were conducted by MEGA 6.0. Results During 2012 to 2017, NoVs were detected in 15.4% (220/1433) of the samples, with high detection rate in children aged 7-12 months (19.2%, 143/746) and in September (27.7%, 33/119). Based on genetic analysis of RdRp, GII.Pe (74.5%%, 137/184) was the most predominating RdRp genotype from 2013 to 2017 while GII.P4 played a dominant role in 2012 (55.6%, 21/36). The most prevalent NoVs genotype was GII.4 (73.6%, 162/220) during 2012 to 2017 among the capsid genotypes. According to genetic analysis of RdRp and capsid sequences, the strains were clustered into 19 RdRp/capsid genotypes, and 12 of them were discordant RdRp and capsid genotypes, such as GII.Pe/GII.4-Sydney_2012, GII.P12/GII.3, GII.P7/GII.6, GII.Pe/GII.3, GII.P16/GII.2. GII.Pe/GII.4-Sydney_2012 was completely instead of the pandemic of GII.P4-2006b/GII.4-2006b since 2013 and distributed across all age groups in children. Conclusions The present study shows high detection rates and genetic diversity of circulating NoVs genotypes in paediatric AGE samples from Shanghai. The findings emphasize the importance of continuous molecular surveillance of emerging NoVs strains.

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