scholarly journals Genetic Diversity and Epidemiology of Norovirus in Children with acute sporadic gastroenteritis in Shanghai, China, 2012-2017

2019 ◽  
Author(s):  
Lijuan Lu ◽  
Huaqing Zhong ◽  
Menghua Xu ◽  
Liyun Su ◽  
Lingfeng Cao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Analysis ◽  
Acute Gastroenteritis ◽  
Dominant Role ◽  
Phylogenetic Analyses ◽  
Age Groups ◽  
Detection Rates ◽  
Children Under Five ◽  
Under Five ◽  
High Detection

Abstract Background Noroviruses are considered the important causes of acute gastroenteritis (AGE) across all age groups especially in children under five years. We investigated the prevalence and molecular epidemiology of norovirus in outpatient children from Children’s Hospital of Fudan University in Shanghai, China. Methods A total of 1433 stool specimens were collected from children under five years with acute gastroenteritis between January 2012 and December 2017. All the samples were analyzed by conventional reverse transcription-polymerase chain reaction (RT-PCR) for genogroup II targeting both the RNA-dependent RNA polymerase (RdRp) and partial capsid genes. Norovirus Genotyping Tool v.2.0 (https://www.rivm.nl/mpf/typingtool/norovirus/) was used for genotyping strains, and phylogenetic analyses were conducted by MEGA 6.0. Results During 2012 to 2017, NoVs were detected in 15.4% (220/1433) of the samples, with high detection rate in children aged 7-12 months (19.2%, 143/746) and in September (27.7%, 33/119). Based on genetic analysis of RdRp, GII.Pe (74.5%%, 137/184) was the most predominating RdRp genotype from 2013 to 2017 while GII.P4 played a dominant role in 2012 (55.6%, 21/36). The most prevalent NoVs genotype was GII.4 (73.6%, 162/220) during 2012 to 2017 among the capsid genotypes. According to genetic analysis of RdRp and capsid sequences, the strains were clustered into 19 RdRp/capsid genotypes, and 12 of them were discordant RdRp and capsid genotypes, such as GII.Pe/GII.4-Sydney_2012, GII.P12/GII.3, GII.P7/GII.6, GII.Pe/GII.3, GII.P16/GII.2. GII.Pe/GII.4-Sydney_2012 was completely instead of the pandemic of GII.P4-2006b/GII.4-2006b since 2013 and distributed across all age groups in children. Conclusions The present study shows high detection rates and genetic diversity of circulating NoVs genotypes in paediatric AGE samples from Shanghai. The findings emphasize the importance of continuous molecular surveillance of emerging NoVs strains.

scholarly journals Genetic Diversity and Epidemiology of Genogroup II Noroviruses in Children with Acute Sporadic Gastroenteritis in Shanghai, China, 2012-2017

2019 ◽  
Author(s):  
Lijuan Lu ◽  
Huaqing Zhong ◽  
Menghua Xu ◽  
Liyun Su ◽  
Lingfeng Cao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Analysis ◽  
Acute Gastroenteritis ◽  
Dominant Role ◽  
Phylogenetic Analyses ◽  
Age Groups ◽  
Detection Rates ◽  
Children Under Five ◽  
Under Five ◽  
Paediatric Age

Abstract Background Noroviruses (NoVs) are considered an important cause of acute gastroenteritis (AGE) across all age groups, especially in children under five years of age. We investigated the epidemiology of noroviruses in outpatient children from the Children’s Hospital of Fudan University in Shanghai, China. Methods Stool specimens were collected between January 2012 and December 2017 from 1433 children under five years of age with acute gastroenteritis. All samples were analysed by conventional reverse transcription-polymerase chain reaction (RT-PCR) for genogroup II NoVs amplifying both the RNA-dependent RNA polymerase (RdRp) and partial capsid genes. The Norovirus Genotyping Tool v.2.0 (https://www.rivm.nl/mpf/typingtool/norovirus/) was used for genotyping the strains, and phylogenetic analyses were conducted by MEGA 6.0. Results From 2012 to 2017, GII NoVs were detected in 15.4% (220/1433) of the samples, with the highest detection rate in children aged 7-12 months (19.2%, 143/746). The seasons with the highest prevalence of GII NoVs infection were autumn and winter . Based on genetic analysis of RdRp, GII.Pe (74.5%%, 137/184) was the most predominant RdRp genotype from 2013 to 2017, while GII.P4 played a dominant role in 2012 (55.6%, 21/36). Among the capsid genotypes, the most prevalent NoV genotype from 2012 to 2017 was GII.4 (73.6%, 162/220). On the basis of genetic analysis of RdRp and capsid sequences, the strains were clustered into 19 RdRp/capsid genotypes, and 12 of them were discordant, such as GII.Pe/GII.4-Sydney_2012, GII.P12/GII.3, GII.P7/GII.6, GII.Pe/GII.3, and GII.P16/GII.2. Starting with 2013, GII.Pe/GII.4-Sydney_2012 had completely replaced the pandemic GII.P4-2006b/GII.4-2006b subtype and was detected in children across all age groups. Conclusions The present study shows high detection rates and the genetic diversity of circulating NoV GII genotypes in paediatric AGE samples from Shanghai. The findings emphasize the importance of continuous molecular surveillance of emerging NoV strains.

scholarly journals Genetic Diversity and Epidemiology of Genogroup II Noroviruses in Children with Acute Sporadic Gastroenteritis in Shanghai, China, 2012-2017

2019 ◽  
Author(s):  
Lijuan Lu ◽  
Huaqing Zhong ◽  
Menghua Xu ◽  
Liyun Su ◽  
Lingfeng Cao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Analysis ◽  
Acute Gastroenteritis ◽  
Dominant Role ◽  
Phylogenetic Analyses ◽  
Age Groups ◽  
Detection Rates ◽  
Children Under Five ◽  
Under Five ◽  
Paediatric Age

Abstract Background Noroviruses (NoVs) are considered an important cause of acute gastroenteritis (AGE) across all age groups, especially in children under five years of age. We investigated the epidemiology of noroviruses in outpatient children from the Children’s Hospital of Fudan University in Shanghai, China. Methods Stool specimens were collected between January 2012 and December 2017 from 1433 children under five years of age with acute gastroenteritis. All samples were analysed by conventional reverse transcription-polymerase chain reaction (RT-PCR) for genogroup II NoVs amplifying both the RNA-dependent RNA polymerase (RdRp) and partial capsid genes. The Norovirus Genotyping Tool v.2.0 (https://www.rivm.nl/mpf/typingtool/norovirus/) was used for genotyping the strains, and phylogenetic analyses were conducted by MEGA 6.0. Results From 2012 to 2017, GII NoVs were detected in 15.4% (220/1433) of the samples, with the highest detection rate in children aged 7-12 months (19.2%, 143/746). The seasons with the highest prevalence of GII NoVs infection were autumn and winter . Based on genetic analysis of RdRp, GII.Pe (74.5%%, 137/184) was the most predominant RdRp genotype from 2013 to 2017, while GII.P4 played a dominant role in 2012 (55.6%, 21/36). Among the capsid genotypes, the most prevalent NoV genotype from 2012 to 2017 was GII.4 (73.6%, 162/220). On the basis of genetic analysis of RdRp and capsid sequences, the strains were clustered into 19 RdRp/capsid genotypes, and 12 of them were discordant, such as GII.Pe/GII.4-Sydney_2012, GII.P12/GII.3, GII.P7/GII.6, GII.Pe/GII.3, and GII.P16/GII.2. Starting with 2013, GII.Pe/GII.4-Sydney_2012 had completely replaced the pandemic GII.P4-2006b/GII.4-2006b subtype and was detected in children across all age groups. Conclusions The present study shows high detection rates and the genetic diversity of circulating NoV GII genotypes in paediatric AGE samples from Shanghai. The findings emphasize the importance of continuous molecular surveillance of emerging NoV strains. Keywords: Norovirus, RdRp/capsid genotypes, Epidemiology, Children, Acute gastroenteritis

scholarly journals Genetic diversity of norovirus in children under 5 years of age with acute gastroenteritis from Angola

2018 ◽  
Vol 146 (5) ◽  
pp. 551-557 ◽  
Author(s):  
A. Esteves ◽  
J. Nordgren ◽  
C. Tavares ◽  
F. Fortes ◽  
R. Dimbu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Acute Gastroenteritis ◽  
Target Population ◽  
Continuous Assessment ◽  
Detection Rates ◽  
Faecal Samples ◽  
High Detection ◽  
Paediatric Age ◽  
Genetic Clusters ◽  
Children Under 5

AbstractNorovirus (NoV) is a major cause of acute gastroenteritis (AGE). In this study, we investigated the genetic diversity of NoV strains identified in children under 5 years of age with AGE in four provinces of Angola. Faecal samples from 343 children were screened for NoV by an in house real-time PCR assay and genotyping was performed by partial capsid gene sequencing. NoV was detected in 17.4% (58/334) of the samples, with high detection rates in children <6 months old (19%) and in children aged 12–24 months (23%). Genotype diversity was large, as demonstrated by the 11 identified genotypes. GII.4 was the predominant genotype (20% of all NoV-positive samples), followed by GII.6 (15%), GI.3 (12%), GII.7 (10%) and by other genotypes to a lesser extent. Two GII.4 variants, New Orleans 2009 and Sydney 2012, were detected and several genetic clusters were observed for genotypes GI.3, GII.6 and GII.7. The present study shows high detection rates and genetic diversity of circulating NoV genotypes in paediatric AGE samples from Angola. This information emphasises the importance of continuous assessment of NoV burden and evolution in the target population.

scholarly journals Stunting on children under five years on family of beneficiary family hope program in Wonogiri district, Central Java

2018 ◽  
Vol 5 (7) ◽  
pp. 2735
Author(s):  
Titus Priyo Harjatmo ◽  
Maria Poppy Herlianty ◽  
Antonius Sri Hartono
Keyword(s):  
Nutritional Status ◽  
Age Groups ◽  
Health Sector ◽  
Conditional Cash Transfer ◽  
Cash Transfer ◽  
Transfer Program ◽  
Infants And Toddlers ◽  
Children Under Five ◽  
Under Five ◽  
Conditional Cash Transfer Program

Background: The nutritional status of infants and toddlers is one indicator of public nutrition, and even has developed into one of the indicators of health and welfare. Basic Health Research (Riskesdas) in 2013 showed that 37,2% children under five suffering from nutritional status is stunting. One of the efforts that have been made by the Indonesian government to reduce malnutrition in infants and toddlers is through a program that is integrated with the health sector namely Conditional Cash Transfer Program (Program Keluarga Harapan). The specific objectives were to identify the characteristics of families recieve Conditional Cash Transfer Program and analysis stunting children under five years in families receiving Conditional Cash Transfer Program in Baturetno subdistric, Wonogiri district.Methods: The study was conducted in the subdistrict of Baturetno, Wonogiri district and has collected a total of 112 infants of families Conditional Cash Transfer Program participants. Sampling of children under five years was done purposively. This study was conducted from July to August 2017.Results: The proportion of children who stunting quite high at 33.0% higher than the results of the Nutritional Status Monitoring in 2016 amounted to 27.5%. If stunting is associated with the age group of stunting problems occur in all age groups in the amount of 31.3% in under 23 months and 34.3% at 23 months upwards of 31.3% children under five short, as much as 25.0% children under five are overweight according to height (weight for height) is normal and thus potentially becoming obese.Conclusions: The implementation of weight monitoring should be monitored the height of children under five in the hope family program.

scholarly journals An ongoing large outbreak of measles in Merseyside, England, January to June 2012

2012 ◽  
Vol 17 (29) ◽  
Author(s):  
R Vivancos ◽  
A Keenan ◽  
S Farmer ◽  
J Atkinson ◽  
E Coffey ◽  
...  
Keyword(s):  
Young Adults ◽  
Age Groups ◽  
Children Under Five ◽  
Under Five ◽  
Large Outbreak

From 1 January to 30 June 2012, 359 confirmed and 157 probable cases of measles were reported in Merseyside, England. The most affected age groups were children under five years and young adults from 15 years of age. Most cases have been sporadic. There have been few outbreaks in nurseries; however, no outbreaks have been reported in schools. Of the cases eligible for vaccination, only 3% of the confirmed cases were fully immunised.

scholarly journals Incidence and Causes of Injury Deaths among Children under Five Years of Age in El-Giza -Egypt: مدى انتشار وأسباب الإصابات المميتة بين الأطفال الذين تقل أعمارهم عن 5 سنوات في الجيزة – مصر

2019 ◽  
Vol 3 (4) ◽  
Author(s):  
Ahmed Mohmed Gad Allah
Keyword(s):  
Child Health ◽  
Rural Areas ◽  
Traffic Accidents ◽  
Age Groups ◽  
Children Under Five ◽  
Under Five ◽  
Number Of Children ◽  
Crude Mortality Rate ◽  
Cause Of Deaths ◽  
One Year

  Objectives: To study the incidence and causes of injury mortality among children under the age of five years in El-Giza. Setting: The study was conducted in El-Giza, Egypt, the second largest city with a population of ~ 8.7 million for 2017. It comprises urban and semiurban settlements. Methods: A descriptive epidemiological study, which obtained information about all deaths using a questionnaire from 197 Health Centres for one year, 2017. Subjects were residents who died from unintentional and intentional injuries. Results: The overall under fives crude mortality rate was 3.1 per1000. The number of children deaths from injuries was 309(8.7% of all deaths), more among males than females (31.5 υ 23.1 per 100 000). Those under 1 had the highest rate, 32.7 per 100 000. The top three causes of deaths were traffic accidents (31.7%), falls (18.8%), and drowning (11%). Conclusions: Injury is the common cause of deaths among children under five years of age in El-Giza. Because all age groups and both sexes are victims of injuries, and most unintentional injuries are preventable, they must be considered as a priority health problem in El-Giza. More studies are needed in rural areas of Egypt. Recommendations: It is important that child health community in Egypt and in other developing countries enhances its focus on injury as a child health issue and integrate injury prevention efforts in child health policies and programmers.   ، ،

scholarly journals Molecular Epidemiology and Clinical Risk Factors of Rotavirus Diarrhea: Single Center 5-Year Experience

2021 ◽  
Author(s):  
Ekin Soydan ◽  
İlknur Çağla ◽  
Mine Düzgol ◽  
Hurşit Apa ◽  
İlker Devrim ◽  
...  
Keyword(s):  
Acute Gastroenteritis ◽  
Laboratory Data ◽  
Clinical Findings ◽  
Study Data ◽  
Children Under Five ◽  
Under Five ◽  
Common Causes ◽  
Training And Research ◽  
Rotavirus Diarrhea

Objective: Acute gastroenteritis due to the rotavirus is one of the common causes of morbidity and mortality in children under five years of age. The objective of this study was to evaluate the epidemiological, clinical, and laboratory data of rotavirus diarrhea in hospitalized children under five years of age. Methods: All children between one month and 60 months old ages who were hospitalized in Health Sciences University Behçet Uz Child Disease and Pediatric Surgery Training and Research Hospital during September 2013 to August 2017 and diagnosed as acute gastroenteritis with rotavirus antigen test positive in feces were included in the current study. Data of the patients were collected retrospectively from medical records. Results: A total of 100 patients were evaluated.The median age of the patients was 13 months (IQR 5-45 months) and 54 patients were male and 46 were female.The highest hospitalization rate was in December, followed by November, and in October. The most common type of G9P (8) serotype was detected in rotavirus serotype analysis by PCR. Breastfeeding infants had milder clinic findings in comparison to the older ones. It was found that clinical findings were milder and Vesicari score was lower in infants who had breast milk. Vesikari score was found to be high in children with severe clinical findings. Conclusion: Rotavirus infection is important for all ages.In case of fever, increased numbers of vomiting and diarrhea, and higher Vesikari system scores may be associated with the severe clinical forms. Determination of rotavirus serotypes and clinical monitoring of genotypic changes are required.

High detection rates and genetic diversity of picobirnaviruses (PBVs) in pigs on St. Kitts Island: Identification of a porcine PBV strain closely related to simian and human PBVs

2020 ◽  
Vol 84 ◽  
pp. 104383
Author(s):  
Soh Jiaying Joycelyn ◽  
Agnes Ng ◽  
Alyssa Kleymann ◽  
Yashpal S. Malik ◽  
Nobumichi Kobayashi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Detection Rates ◽  
High Detection

Genetic analysis of noroviruses taken from gastroenteritis patients, river water and oysters

2004 ◽  
Vol 50 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Y. Ueki ◽  
K. Akiyama ◽  
T. Watanabe ◽  
T. Omura
Keyword(s):  
Genetic Diversity ◽  
Genetic Analysis ◽  
Rna Polymerase ◽  
River Water ◽  
Cell Cultures ◽  
Health Problem ◽  
Acute Gastroenteritis ◽  
Aquatic Environment ◽  
Rna Dependent Rna Polymerase ◽  
Bacterial Agent

As oysters are eaten raw in Japan, their contamination with the non-bacterial agent of gastroenteritis has become a serious health problem. As it is well known that oysters tend to concentrate noroviruses (NV) in their digestive diverticula, NV may be linked with the acute gastroenteritis. However, since NV cannot be cultivated in cell cultures, and they have genetic diversity, the behaviour of NV in the aquatic environment is little known. In this study, NV samples were taken from gastroenteritis patients; from the river flowing into the oyster-farming area; and from oysters harvested from that river. Genetic identities of NV samples were analysed in capsid and RNA-dependent RNA polymerase (RdRp) regions respectively. In both regions, strains taken from patients were &gt;96% identical with those from river and oyster samples. This proved that oysters were contaminated with NV excreted from patients with gastroenteritis.

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