scholarly journals PO 8524 MOLECULAR CHARACTERISATION OF THE NOROVIRUS STRAINS RESPONSIBLE FOR ACUTE DIARRHOEA IN CHILDREN UNDER FIVE YEARS OLD HOSPITALISED IN BRAZZAVILLE

2019 ◽  
Vol 4 (Suppl 3) ◽  
pp. A49.2-A49
Author(s):  
Vivaldie E Mikounou Louya ◽  
Félix Koukouikila-Koussounda ◽  
Christevy Vouvoungui ◽  
Simon Ch. Kobawila ◽  
Francine Ntoumi

BackgroundAcute gastroenteritis is a leading cause of morbidity and mortality among children under five years old worldwide. Viruses are the most common responsible agent and norovirus is second after rotavirus. There is no published data on the occurrence of this agent in Brazzaville. This study aimed to determine the prevalence of norovirus infection and’to evaluate the risk factors in hospitalised children in Brazzaville, Congo.MethodsFrom June 2012 to June 2013, stool samples were collected from children under five years old hospitalised with acute gastroenteritis at Makelekele hospital. Rotavirus and adenovirus infections were already characterised in this population. A total of 545 samples were tested for GI and GII norovirus infections using nested duplex reverse-transcription–polymerase chain reaction with specific primers. The positive samples will be sequenced and analysed to determine the corresponding genotype.ResultsThe GI and GII norovirus infection were found in 148 samples (27, 14%) in this study. Males (28, 85%) were more infected than females (25%) but the difference was not significant. Norovirus infection was detected only in children under 24 months with a higher prevalence in the age group of 7–12 months (p value=0,048). The norovirus infection was detected throughout the year, but it peaked during the dry season (August-September). Dual infection of rotavirus and norovirus was detected in 65 cases (11, 9%), rotavirus-adenovirus in 8 cases (1, 5%), norovirus-adenovirus in 4 cases (0, 73%). Triple infection was detected in 3 cases (0, 55%).ConclusionThis study suggests that norovirus infection is the second cause of gastroenteritis after rotavirus in the study area. However, further surveillance investigations need to be pursued in other sentinel sites of the country.

2019 ◽  
Vol 31 (1) ◽  
pp. 87-92

Rotaviruses are regarded as the most common cause of viral gastroenteritis and are responsible for considerable morbidity and mortality among children especially under five years of age worldwide. In developing countries like Myanmar, where diarrhoea is in the priority childhood disease, rotavirus surveillance and detection of rotavirus genotypes are utmost important. A hospital-based, cross-sectional descriptive study was conducted at Yangon Children‟s Hospital among under five children admitted for acute diarrhoea from January to October 2016. This study includes detection of Group A rotavirus antigen by commercial enzyme-linked immunosorbent assay (ELISA) and genotyping by multiplex RT-PCR. From a total of 488 collected samples, rotavirus antigen was detected in 219 samples (45%). Rotavirus diarrhoea was most common among the age of 6-11 months (38.8%) followed by 12-23 months (37.9%). The results showed that boys were more commonly affected than girls. Detection of rotavirus positivity was peak in February (57.6 %). Out of 219 stool samples with positive ELISA result, 40 stool samples with high optical density value were proceeded for further determination of G and P genotypes. Regarding distribution of G genotypes, the most common G genotype was G9 which comprised 45%, and that of P genotype was P[8] which comprised 92.5%. Regarding combination of G and P genotypes, the most frequent combination is G9P[8], and it constituted 42.5%. Untypable genotypes were seen in 30% of G and 2.5% of P typing. As rotavirus infection can be prevented by vaccine, WHO recommended that rotavirus vaccination should be included in national immunization program especially in countries where prevalence of rotavirus is high. The distribution of G and P genotypes is important in consideration of appropriate vaccine in pre-vaccination and evaluation of effectiveness of vaccine in post-vaccination period. Therefore, the information on currently circulating genotypes of rotavirus in this study will serve as valuable data for vaccination programme.


2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Eti Kurniawati

<p>Ascariasis incidence in children under five in the region work of Puskesmas Olak Kemang still a health problem that needs to be addressed, with a percentage of 51.0% is higher than in other wilayh. The disease is not lethal but can undermine the health of the human body so that the resulting decline in nutritional status, decreased intelligence and brain power or immune health in children.</p><p>               This research is a quantitative study using descriptive analytic method with cross sectional study design that aims to determine the relationship between the independent variables and the dependent variable. The population in this research that all children under five in the area of Puskesmas Olak Kemang, samples in this study were 75 children under five. Data were analyzed using analysis Univariate and Bivariate analysis with Chi-Square Test.</p><p>               The results showed that 78.0% of mothers of children under five who are not air personal hygiene, 60.4% of mothers of children under five unusual CTPS, 82.1% of respondents who did not state house meliliki latrine / WC. The results of the bivariate analysis showed that there was a significant relationship between personal hygiene (p-value = 0.000), customs CTPS (p-value = 0.001), latrine ownership (p-value = 0.000) with the incidence of intestinal worms in Puskesmas Olak Kemang Jambi.</p><p>               In connection with the results obtained, that the danger of de- worming is dependent on the cleanliness of his mother in serving as personal hygiene, CTPS and Owners toilets in every home. Thus the researchers suggested that the health center may be able to provide information to the public education about the dangers of de- worming to create a healthy society.</p><p> </p>Keyword                     :  Behavior, Children Events


2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Kennedy Diema Konlan ◽  
Milipaak Japiong ◽  
Kennedy Dodam Konlan ◽  
Agani Afaya ◽  
Solomon Mohammed Salia ◽  
...  

Background. In Ghana, attempts to control malaria through antimalarial medications are currently threatened by the emergence and spread of drug resistant malaria parasites. This, together with the increasing incidence of malaria, has heightened the need for a more effective method of controlling the spread. The use of Insecticide Treated Bed Net (ITN) has been recognised as an effective measure in the prevention of malaria. Objective/Purpose. In this study, we examined the utilisation of ITN among caregivers of children under five years in Ho municipality of Ghana. Methods. This descriptive cross-sectional study recruited 283 household representatives through a multistage sampling method. A questionnaire was used to collect the data and was analyzed using STATA version 14. Descriptive and inferential statistics were adopted in presenting the data. Results. Ownership of ITN was higher (80.7%) than its utilisation (41.7%). The age of caregiver was strongly associated with the utilisation of ITN (AOR=2.00, 95% CI=0.00, 0.02, p<0.001) among children less than five years. Caregivers aged 26-35 were 49% times less likely to use an ITN as compared to those aged between 17 and 25 and the difference was statistically significant. Conclusion. In order to ensure a high ITN coverage and utilisation, there is the need for continuous distribution of ITNs to households. Households should be sensitized to use the nets to prevent the continuous spread of malaria.


2020 ◽  
Vol 7 (1) ◽  
pp. 28
Author(s):  
Kasman Kasman ◽  
Nuning Irnawulan Ishak

ABSTRAKSetiap anak mengalami episode serangan diare rata-rata 3,3 kali setiap tahun. Lebih kurang 80% kematian terjadi pada anak berusia kurang dari dua tahun. Penyakit diare merupakan salah satu penyakit yang banyak terjadi di Kota Banjarmasin. Penyebabnya diduga karena Kondisi sanitasi lingkungan yang tidak baik. Penelitian ini bertujuan untuk menganalisis penggunaan jamban terhadap kejadian diare pada anak balita di Kota Banjarmasin. Rancangan penelitian ini menggunakan desain cross-sectional. Populasi penelitian adalah seluruh anak balita yang ada di Kota Banjarmasin sebanyak 54.746 balita. Teknik penarikan sampel secara multistage sampling dengan jumlah sampel sebanyak 188 balita. Pengumpulan data dilakukan pada Bulan Juni sampai Agustus 2018 dengan wawancara langsung pada responden menggunakan kuesioner. Analisis data menggunakan uji statistik Chi Square. Hasil penelitian ini menunjukkan bahwa terdapat 22,9% anak balita yang menderita diare. Sebagian besar (97,9%) responden telah memiliki Jamban dengan jenis jamban menggunakan tangki septic 94,6%. Terdapat 19% jamban dengan kondisi yang tidak baik. Terdapat hubungan yang bermakna antara kepemilikan jamban (p-value=0,038) dan kondisi jamban (p-value =0,000) terhadap kejadian Diare pada Balita di Kota Banjarmasin. Diharapkan kepada masyarakat untuk memperhatikan kebersihan jamban.Kata-kata kunci : Diare, Balita, Penyakit infeksi, JambanABSTRACTEach child experiences episodes of diarrhea attack an average of 3.3 times each year. Approximately 80% of deaths occur in children aged less than two years. Diarrhea is one of the many diseases that occur in Banjarmasin. The cause is thought to be due to poor environmental sanitation. This study aims to analyze the use of latrines on the incidence of diarrhea in under-five children in Banjarmasin. The design of this study used a cross-sectional design. The study population was all toddlers in Banjarmasin of 54,746 toddlers. The sampling technique was multistage sampling with a total sample of 188 toddlers. Data collection was conducted from June to August 2018 with an interview with the respondent directly using a questionnaire. Data analysis using Chi-Square statistical tests. The results of this study indicate that there were 22.9% of children under five suffering from diarrhea. Most (97.9%) of respondents have had latrines in the type of latrine using a 94.6% septic tank. There is a significant relationship between latrine ownership (p-value= 0.038) and latrine condition (p-value= 0,000) to the incidence of diarrhea in children under five in Banjarmasin City. It is expected the public to pay attention to hygiene latrine.Keywords: Diarrhea, under-five children, infectious diseases, latrines


2020 ◽  
Vol 30 (6) ◽  
Author(s):  
G.E. Kpene ◽  
S.Y. Lokpo ◽  
J.G. Deku ◽  
E. Agboli ◽  
P.K. Owiafe

BACKGROUND፡ The study investigated intestinal parasitic infestations (IPIs) and possible risk factors associated with asymptomatic children under five (5) years in five (5) selected communities in the Ho Municipality.METHODS: The study design was cross- sectional, with a simple random sampling technique involving 150 asymptomatic children under 5 years from 5 selected communities (Klave, Hoe, Freetown, Dave and Godokpe) in the Ho Municipality. A questionnaire was used to obtain socio-demographics and other relevant parameters. Direct wet preparation, formol-ether concentration and Modified ZN staining techniques were used for the identification of intestinal parasites from participants’ stool samples. The Fisher’s exact test and binary logistic regression analysis were used to determine the difference in IPIs proportions and assess the risk factors associated with IPIs respectively.RESULTS: The overall IPIs cases was 14% (21/150). Cryptosporidium spp was most predominant [5.3% (8/150)], followed by Entamoeba spp [3.3% (5/150)], Cyclospora cayetenensis [2.7% (4/150)], Ascaris lumbricoides [1.3% (2/150)], Giardia lamblia [0.7% (1/150)] and Strongyloides stercoralis [0.7% (1/150)]. Children in rural communities (23.4%) recorded significantly higher case rate compared to those in urban communities (9.8%0), (p=0.04). Lower educational attainment of mother [OR=0.55, 95% CI (0.37 – 0.83), p-value = 0.015] andresidence in rural communities [OR = 0.53, 95% CI (0.33 –0.88)], p-value = 0.025] were significantly associated with IPIs.CONCLUSION: Asymptomatic IPIs are quite prevalent among children under 5 years in the Ho Municipality. The study thus recommends active sensitization programs for parents/guardians on preventive measures and school health programs should be instituted in rural communities.


2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Ana Samiatul Milah ◽  
Ade Zaqiah

Nutrition / nutrition problems occur in every life cycle and throughout the life cycle of women, starting from the womb (fetus), infants, children, adults and old age. The period of the first two years of life is a critical period, because during this period there was very rapid growth and development. Nutritional disorders that occur in this period are permanent, cannot be recovered even though nutritional needs in the next period are met. Data obtained from the Cikoneng Health Center in Cikoneng Sub-district of Ciamis District in 2017 in January the nutritional status of children under five was obtained, but the total number of malnourished sufferers was 29 children. Then in 2018 experienced an increase of 46 children consisting of 42 undernourished children and 4 malnourished children out of 789 children who could be measured and in 2019 there were 213 people. The method used in this research is analytic descriptive research method. The population in this study were all mothers and toddlers aged 3-5 years in Sindangsari Village, Cikoneng District, Ciamis Regency amounted to 213 people. The sampling technique used was using random sampling technique. The results of this study were the results of research of 68 respondents, mother's education in the secondary education category with good nutritional status in children as many as 15 (62.5%) respondents. Hypothesis test results using chi square obtained p value of 0.014 smaller than alpha 0.05. From the results of this study it can be concluded that there is a relationship between maternal education and nutritional status of children aged 3-5 years in Sindangsari Village, Cikoneng District, Ciamis Regency with a p-value of 0.014 smaller than alpha = 0.05. The results of the study are expected to contribute scientifically to the improvement of knowledge related to child growth and development problems and can be used as information to increase knowledge about the importance of child development for the community in order to improve the health and welfare of mothers and children, can also be for children to be healthy in terms of nutritional intake, good health status from nutritional status and health assessment, one of the steps in efforts to conduct nutrition counseling to the community, especially mothers who have children under five at the age of 3-5 years.


2019 ◽  
Vol 5 (2) ◽  
pp. 66-74
Author(s):  
Tutik Hidayati

The problem of malnutrition in children under five is still a major health problem in the world, including Indonesia. Indonesia is a developing country that still faces considerable problems of malnutrition. Nutritional problems nationally are under-five and under-nutrition children under five. Poor nutritional status is a condition of the body experiencing nutritional deficiencies or below standard. Based on the nutritional status monitoring (PSG) survey in Probolinggo District, there was an increase in cases of malnutrition, obtained in the prevalence of non-compliance with the weight per year in 2017 of 9.44%, an increase compared to 2016, namely 8.65% under-five children under five. Toddlers are considered the biggest nutritional risk because patterns of poor feeding have an impact on growth and development. This causes the toddler age group to increase the risk of morbidity and mortality. Based on a preliminary study in April 2018, data was obtained that Probolinggo was ranked second in the area of ​​malnutrition in East Java. In 2017 there are 130 toddlers who experience malnutrition in the Kalibuntu Coast of Probolinggo Regency. The cause of malnutrition is due to the pattern and intake of toddlers' food which contains less nutrition.The purpose of this study was to analyze the effect of nutritional support programs on feeding patterns and nutritional status of the Kalibuntu Coastal Children in Probolinggo Regency. This research is an experimental study, namely by measuring the increase / comparison of feeding patterns and nutritional status before and after the nutrition companion program. The population in this study were all toddlers with malnutrition in the Kalibuntu Coast of 130 people in Probolinggo Regency. Sampling uses Total Sampling. Samples are all mothers who have underweight and malnourished children under the age of 130 people in the Kalibuntu Coast of Probolinggo Regency. Data collection using observation sheets and interviews. Analysis of univariate data using frequency distribution, bivariate analysis using Wilcoxon match paired test and multivariate analysis using multiple linear regression then drawn conclusions. The results of the Wilcoxon match paired test between nutritional companions on feeding patterns obtained a p value of 0,000 <α 0,05, so ha was accepted so that there was an effect of nutritional companion on feeding patterns. The value of Exp (B) in the logistic regression analysis of eating parenting has a value of 85.008, which means that parenting has a chance of 85,008 times to change. While the nutritional status variable in multiple logistic regression has an Exp (B) value of 3.663, from these results it can be explained that nutritional status has an opportunity to increase 3.663 times.


2020 ◽  
Vol 11 (2) ◽  
pp. 59-70
Author(s):  
Ami Ami Oetamiati Wiharjo

Febrile convulsion (febris convulsion/stuip/step) i.e. seizures that arise at the time of fever not caused by processes in the head (brain : like meningitis or inflammation of the lining of the brain, ensifilitis or brain inflammation) but outside the head for example because of an infection in the respiratory tract, ear or infection in the digestive tract. Usually experienced by children aged 6 months to 5 years. In West Java Province in 2012 patients with febrile seizures in the Hospital amounted to 2,220 for ages 0-1 years, while there are 5,696 for ages 1-4 years. This study aims to determine the correlation between the level of knowledge of parents with first aid febrile seizures in children under five in the Aster room of Bogor Hospital in 2018. The type of research used is analitik korelatif with research design cross sectional. Sampling in this study with total sampling techniques a sample of this research is 35 respondents. Data collection was obtained through questionnaires in the form of closed questionnairestotaling 20 statements about first aid knowledge of febrile seizures and 14 questions about first aid febrile seizures. Analysis of the data used is univariat and bivariat (Chi-Square). Based on the knowledge of febrile seizures there were 21 (60.0%) respondents with good knowledge. Based on first aid febrile seizures in children under five there were 22 (62.9%) respondents with positive actions. Of the 35 respondents there were 18 (51.4%) respondents who had a good level of knowledge with positive first aid measures in febrile seizures. The results of bivariate analysis used an analysis test Chi-Square earned value p value 0,002≤0,05 (alpha), meaning Ha is accepted and H0 is rejected. Shows that is a correlation between the level of knowledge of parents and first aid in febrile seizures in children under five in the Aster of Bogor Hospital in 2018.


2019 ◽  
Vol 1 (2) ◽  
pp. 90-97
Author(s):  
Christine Vita Gloria Purba ◽  
Okta Safryanni ◽  
Al Hidayati ◽  
Zulmeliza Rasyid

Non pneumonia Acute Respiratory Tract Infection (ARI) is a respiratory tract disease that is acute with a cough that does not show symptoms of increased respiratory frequency and does not indicate the pull of the lower chest wall inward. The incidence of non pneumonia ARI  in children under five in Kedung Sari Village was recorded at 40.06%. This study aims to determine the description and determinant of the incidence of non-pneumonia ARI  in children under five in Kedung Sari Village, Sukajadi District, Pekanbaru City in 2019. This type of research is quantitative observational analytic with cross sectional design. The study population was all children under five who lived in Kedung Sari Village, Sukajadi District, Pekanbaru City, amounting to 312 people with a sample of 172 respondents. The sampling technique was done by consecutive sampling. The type of data used in this study are primary data and secondary data by means of retrieving data through measurements and questionnaires. Data analysis was done by univariate and bivariate with chi square test. The results showed that there was a correlation between exposure to cigarette smoke (p-value 0,000) and the incidence of non-pneumonia ARI in children under five. It is expected that health workers, especially in the health promotion section of the community health center in implementing control and eradication programs of Acute Respiratory Tract Infection can provide health education, especially regarding the dangers of cigarette smoke for children under five and recommend smokers to smoke in special places so children under five are not exposed cigarettes.


2021 ◽  
Vol 9 (E) ◽  
pp. 373-377
Author(s):  
Rina Tampake ◽  
Ros Arianty ◽  
Selvi A . Mangundap ◽  
Baiq Emy ◽  
Hanum Sasmita

AIM: This study aimed to determine the effectiveness of training in improving health cadres’ ability in early detection and risk factors of stunting in toddlers. METHODS: The research method used was a quasi-experimental non-randomized pre- and post-test only control design. The independent variable was cadre training. The training was conducted through learning activities on health cadres using lecture method, question and answer method, and brainstorming accompanied by training booklets/modules and demonstrations. The dependent variable was knowledge, attitudes, and skills in detecting stunting and risk factors for stunting in children under-five. There were 53 health cadres as samples scattered in the Tomini Community Health Center with the purposive sampling technique. The data analysis technique used was a parametric statistical paired sample t-test. RESULTS: Training of health cadres effectively increased health cadres’ ability to detect stunting and risk factors for stunting in children under-five. The different test results for each variable that showed the pre-post-test p-value for knowledge, attitudes, and health cadres’ skills were 0.000, smaller than the significance level of 0.05 (p < 0.05). Besides, the mean score of each variable obtained after training for health cadres was more significant than before training, including knowledge 17.392 >12.264, attitudes 33.603 >27.226, and skills 90.019 >62113. Those are means that cadres’ training effectively increased the knowledge, attitudes, and skills of health cadres in detecting stunting and risk factors for stunting in children under-five. CONCLUSION: Training of health cadres effectively increases the knowledge, attitudes, and skills of health cadres about early detection and risk factors of stunting in the working area of Tomini Public Health Centre, Parigi Moutong Regency.


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