Toxicological evaluation of ethanolic root extract of Morinda lucida (L.) Benth. (Rubiaceae) in male Wistar rats

2011 ◽  
Vol 2 (2) ◽  
pp. 108 ◽  
Author(s):  
Anofi OmotayoTom Ashafa ◽  
OlugbengaOlatunde Olunu
Keyword(s):  
Wistar Rats ◽  
Root Extract ◽  
Toxicological Evaluation ◽  
Male Wistar Rats

scholarly journals Hepatic Enzyme Effects of an Imperata cylindrica Extract

2021 ◽  
pp. 32-40
Author(s):  
M. O. Nwokike ◽  
S. I. Ghasi ◽  
E. C. Ogbuagu ◽  
M. N. Ezenwaeze ◽  
Akpotu E. Ajirioghene
Keyword(s):  
Wistar Rats ◽  
Intraperitoneal Administration ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Imperata Cylindrica ◽  
Root Extract ◽  
Hepatic Enzyme ◽  
Male Wistar Rats ◽  
Group 5 ◽  
Group 2

This study was performed to investigate the effects of aqueous Imperata cylindrica root extract on hepatic enzyme levels of alloxan-induced diabetic male Wistar rats. Forty (48) male wistar rats were divided into six groups consisting of eight animals each. Diabetes mellitus was induced using intraperitoneal administration 150 mg/kg body weight of alloxan and treatment was carried out for a period of 28 days. The first group served as the normal control and received only feed and water ad libitum. In Group 2 were diabetic rats without treatment with extracts. Group 3: diabetic rats treated with 200 mg/kg aqueous Imperata cylindrica root extract. Group 4: diabetic rats treated with 400mg/kg aqueous Imperata cylindrica root extract. Group 5: diabetic rats treated with 600mg/kg ethanol extract of aqueous Imperata cylindrica root extract. While Group 6 was diabetic rats treated with 0.5mg/kg Glibenclamide. The liver enzymes alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase levels were significantly (p < 0.05) changed in rats treated with Alloxan (150mg/kg b.w.) while treatment with the respective dosages of extracts significantly changed the levels of these parameters to normal. The results obtained indicate that the different doses of aqueous Imperata cylindrica root extracts were beneficial in mending damages to the liver caused by Alloxan monohydrate in the male wistar rats.

Anticonvulsive and anti-epileptogenesis effects of Echinacea purpurea root extract, an involvement of CB2 receptor

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Masoumeh Gholami ◽  
Jamal Amri ◽  
Saeed Pazhoohan ◽  
Mehdi Sadegh
Keyword(s):  
Mortality Rate ◽  
Receptor Antagonist ◽  
Wistar Rats ◽  
Echinacea Purpurea ◽  
Root Extract ◽  
Cb2 Receptor ◽  
Kindling Model ◽  
Male Wistar Rats ◽  
Clonic Seizures ◽  
Cb2 Receptors

Abstract Objective Phytocannabinoids beyond the Δ9-tetrahy-drocannabinol have shown anticonvulsive effects. Also, alkylamides from Echinacea purpurea have been proved as cannabinomimetics. We examined the effect of the hydroalcoholic root extract of E. purpurea on pentylenetetrazol (PTZ)-induced tonic–clonic seizures and kindling model of epileptogenesis and the involvement of CB2 receptors as the mediator of this effect. Methods Male Wistar rats (200 ± 20 g) were used. Single intraperitoneal (i.p.) injection of PTZ (80 mg/kg) was used to induce tonic–clonic seizures. The kindling model of epileptogenesis was induced by daily injections of PTZ (37 mg/kg; i.p. for 15 days). Latency and duration of the stages were monitored for analysis. The hydroalcoholic root extract of E. purpurea was injected (i.p.) 20 min before seizure induction at the doses of 10, 50, 100 and 200 mg/kg. CB2 receptor antagonist SR144528 was injected (0.1 mg/kg; i.p.) 20 min before the Echinacea injection. Results In the tonic–clonic model, pretreatment with E. purpurea at the doses of 100 and 200 mg/kg significantly increased latencies to S2–S6, while it significantly decreased S6 duration and mortality rate. SR144528 injection before the injection of 100 mg/kg of E. purpurea significantly prevented the effects of the extract on S4–S6 latencies. In the kindling model, E. purpurea at the doses of 100 and 200 mg/kg significantly delayed epileptogenesis and decreased mortality rate, while SR144528 injection before the injection of 100 mg/kg of E. purpurea significantly blocked this effect of the extract. Conclusion These findings revealed the anticonvulsive and antiepileptogenesis effects of the E. purpurea root extract, which can be mediated by CB2 receptors.

scholarly journals Characterization of Hippocampal Histomorphology Following R. vomitoria Root Extract Treatment

2020 ◽  
pp. 1-19
Author(s):  
Moses B. Ekong ◽  
Ubong Ekpene ◽  
Agnes A. Nwakanma ◽  
Christopher C. Mbadugha
Keyword(s):  
Wistar Rats ◽  
Brain Activity ◽  
Neuron Specific Enolase ◽  
Bark Extract ◽  
Control Group ◽  
Average Weight ◽  
Root Extract ◽  
Root Bark ◽  
Male Wistar Rats ◽  
Deep Anaesthesia

Rauwolfia vomitoria Afzel. is an antipsychotic plant used by several African communities in the management of psychiatric conditions with good outcomes. Concerns about its dosages on brain activity lead to this investigation of its action on the hippocampal microstructure. Twenty-four adult male Wistar rats of average weight 200 g, were assigned into four groups (n = 6): control; 200, 300 and 400 mg/kg body weight of RV root bark extract, respectively. The administration was once daily, and orally for seven days. Daily observation of the animals was done till on day eight when they were sacrificed after deep anaesthesia. Each brain was processed for histology and immunohistochemical studies. Animals in the 200, 300 and 400 mg/kg RV groups appeared generally dull and drowsy, and barely fed. Their hippocampal histology showed neuronal atrophy and karyorrhexis, with no difference in cell count, although the pyramidal cell numbers decreased in the 300 and 400 mg/kg RV groups. Neuron-specific enolase decreased in the 400 mg/kg RV group, while neurofilament decreased in all test groups. Glial fibrillary acidic protein expression and density increased in the 200 and 300 mg/kg RV groups, but not the 400 mg/kg RV group, all compared with the control group. The given doses of RV root bark extract in adult Wistar rats showed sedative activities with hippocampal histopathological changes, which may not be reversible, thereby leading to the hippocampal functional deficit.

scholarly journals Efficacy and toxicological evaluation of Coccinia grandis (Cucurbitaceae) extract in male Wistar rats

2013 ◽  
Vol 3 (6) ◽  
pp. 460-466 ◽  
Author(s):  
Anoja Priyadarshani Attanayake ◽  
Kamani Ayoma Perera Wijewardena Jayatilaka ◽  
Chitra Pathirana ◽  
Lakmini Kumari Boralugoda Mudduwa
Keyword(s):  
Wistar Rats ◽  
Toxicological Evaluation ◽  
Coccinia Grandis ◽  
Male Wistar Rats

Adverse reproductive effects of ethanolic root extract of Waltheria indica in male Wistar rats

2019 ◽  
Vol 16 (4) ◽  
Author(s):  
Afisu Basiru ◽  
Ganiu Jimoh Akorede ◽  
Kehinde Soetan ◽  
Funsho O. Olayemi
Keyword(s):  
Adverse Effect ◽  
Wistar Rats ◽  
Sperm Count ◽  
Ethanolic Extract ◽  
Reproductive Hormones ◽  
Male Reproduction ◽  
Sperm Parameters ◽  
Control Group ◽  
Root Extract ◽  
Male Wistar Rats

Abstract Background Numerous uses of Waltheria indica plant such as antitrypanosomal, antibacterial and antimalarial effects have been reported. It has however been reported that most plants with antibacterial and antiprotozoal effects have adverse effect on male reproduction. Hence, we evaluated the effect of Waltheria indica root on male reproductive parameters. Methods Twenty adult male Wistar rats were randomly divided into four groups (n=5); A–D. Group A served as control group while groups B, C and D were administered with 200, 400 and 800 mg/Kg body weight of crude ethanolic extract of Waltheria indica root. After 28 days of administration, the rats were sacrificed and sperm parameters, sperm morphology, serum reproductive hormones and lipids were determined. Results There was a significant reduction in sperm count and motility as well as significant increase in percentage abnormal sperm cell (p<0.001) at the 400 and 800 mg/kg BW. The serum levels of testosterone was also significantly reduced while total cholesterol increased significantly (p<0.05) at the highest dose. Conclusion Waltheria indica root has adverse effect on male reproduction through reduction in sperm parameters and male reproductive hormones.

scholarly journals Anti-fertility effects of fractions from Carica papaya (Pawpaw) Linn. methanol root extract in male Wistar rats

2019 ◽  
Vol 12 (7) ◽  
pp. 1563-1568 ◽  
Author(s):  
Chinaka O. Nwaehujor ◽  
Julius O. Ode ◽  
Mercy R. Ekwere ◽  
Rita I. Udegbunam
Keyword(s):  
Wistar Rats ◽  
Carica Papaya ◽  
Root Extract ◽  
Male Wistar Rats

Anti-inflammatory and antipyretic properties of Corchorus olitorius aqueous root extract in Wistar rats

2015 ◽  
Vol 26 (4) ◽  
Author(s):  
Bamidele V. Owoyele ◽  
Aboyeji L. Oyewole ◽  
Modupe L. Alimi ◽  
Shukurat A. Sanni ◽  
Sabitiu A. Oyeleke
Keyword(s):  
Aqueous Extract ◽  
Normal Saline ◽  
Wistar Rats ◽  
Root Extract ◽  
Corchorus Olitorius ◽  
Reference Groups ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
Aqueous Root Extract

AbstractThis study was designed to provide information about the antipyretic and anti-inflammatory effects ofThirty male Wistar rats were divided into six groups of five animals each; the control and reference groups were administered normal saline (10 mL/kg) and indomethacin (5 mg/kg), respectively, whereas the remaining four groups were administered aqueous extract ofThe results showed that

scholarly journals Differential Influence of Pueraria lobata Root Extract and Its Main Isoflavones on Ghrelin Levels in Alcohol-Treated Rats

2021 ◽  
Vol 15 (1) ◽  
pp. 25
Author(s):  
Michał Szulc ◽  
Radosław Kujawski ◽  
Justyna Baraniak ◽  
Małgorzata Kania-Dobrowolska ◽  
Ewa Kamińska ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Alcohol Intake ◽  
Root Extract ◽  
Alcohol Tolerance ◽  
Pueraria Lobata ◽  
Elisa Method ◽  
Tolerance Development ◽  
Ghrelin Concentration ◽  
Male Wistar Rats ◽  
Kudzu Root Extract

The study was carried out on alcohol-preferring male Wistar rats. The following drugs were repeatedly (28×) administered: acamprosate (500 mg/kg, p.o.), naltrexone (0.1 mg/kg, i.p), and Pueraria lobata (kudzu) root extract (KU) (500 mg/kg, p.o.) and its isoflavones: daidzin (40 mg/kg, p.o.) and puerarin (150 mg/kg, p.o.). Their effects on a voluntary alcohol intake were assessed. KU and alcohol were also given for 9 days in an experiment on alcohol tolerance development. Finally, total and active ghrelin levels in peripheral blood serum were measured by ELISA method. Acamprosate, naltrexone, daidzin, and puerarin, reducing the alcohol intake, caused an increase in both forms of ghrelin levels. On the contrary, though KU inhibited the alcohol intake and alcohol tolerance development, it reduced ghrelin levels in alcohol-preferring rats. The changes of ghrelin concentration could play a role as an indicator of the currently used drugs. The other effect on the KU-induced shift in ghrelin levels in the presence of alcohol requires further detailed study.

Sign in / Sign up

Export Citation Format

Share Document