Patterns of nucleolar organizer region in urinary bladder cancer in view of 2004 WHO classification of urothelial neoplasms

Abstract Background Cancer is the leading cause of death with 7.6 million deaths worldwide annually. About 19% of bladder cancer cases are attributed to environmental and occupational factors. The knowledge of any disease is very vital in the control or prevention of such disease. This study examined the knowledge and perception of urinary bladder cancer among 104 local dye workers in Ghana (Kumasi, Koforidua and Somanya). Methods A well-structured questionnaire was used to measure participants’ knowledge and perception towards bladder cancer. Forty out of the 104 participants were recruited to provide their urine samples for cytomorphological study. Thin smears were prepared and stained with Papanicolaou stain. Respondents were selected based on clinical history and working experience. Result The response rate to the questionnaires was 100% of the 104 respondents interviewed. The respondents had a mean age of 26 years. Overall, about 10% of the respondents in this study had good knowledge levels on bladder cancer and 16% had good perception of the disease. There was a significant correlation between knowledge and educational level of the participants (p < 0.001). Report of the cytomorphological study indicates that none of the samples of the participants screened had cancerous cells. Conclusion The findings suggest that level of knowledge about bladder cancer among local dye workers in this study was low, while their level of perception was just above average.

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Comparative FISH analysis of Senna tora tandem repeats revealed insights into the chromosome dynamics in Senna

Genes & Genomics ◽

10.1007/s13258-021-01051-w ◽

2021 ◽

Vol 43 (3) ◽

pp. 237-249 ◽

Cited By ~ 2

Author(s):

Thanh Dat Ta ◽

Nomar Espinosa Waminal ◽

Thi Hong Nguyen ◽

Remnyl Joyce Pellerin ◽

Hyun Hee Kim

Keyword(s):

Tandem Repeats ◽

Chromosomal Rearrangements ◽

Organizer Region ◽

Nucleolar Organizer Region ◽

Nucleolar Organizer ◽

Pericentromeric Region ◽

Its2 Sequence ◽

Fish Analysis ◽

Chromosomal Distribution ◽

Chromosome Dynamics

Abstract Background DNA tandem repeats (TRs) are often abundant and occupy discrete regions in eukaryotic genomes. These TRs often cause or generate chromosomal rearrangements, which, in turn, drive chromosome evolution and speciation. Tracing the chromosomal distribution of TRs could therefore provide insights into the chromosome dynamics and speciation among closely related taxa. The basic chromosome number in the genus Senna is 2n = 28, but dysploid species like Senna tora have also been observed. Objective To understand the dynamics of these TRs and their impact on S. tora dysploidization. Methods We performed a comparative fluorescence in situ hybridization (FISH) analysis among nine closely related Senna species and compared the chromosomal distribution of these repeats from a cytotaxonomic perspective by using the ITS1-5.8S-ITS2 sequence to infer phylogenetic relationships. Results Of the nine S. tora TRs, two did not show any FISH signal whereas seven TRs showed similar and contrasting patterns to other Senna species. StoTR01_86, which was localized in the pericentromeric regions in all S. tora, but not at the nucleolar organizer region (NOR) site, was colocalized at the NOR site in all species except in S. siamea. StoTR02_7_tel was mostly localized at chromosome termini, but some species had an interstitial telomeric repeat in a few chromosomes. StoTR05_180 was distributed in the subtelomeric region in most species and was highly amplified in the pericentromeric region in some species. StoTR06_159 was either absent or colocalized in the NOR site in some species, and StoIGS_463, which was localized at the NOR site in S. tora, was either absent or localized at the subtelomeric or pericentromeric regions in other species. Conclusions These data suggest that TRs play important roles in S. tora dysploidy and suggest the involvement of 45S rDNA intergenic spacers in “carrying” repeats during genome reshuffling.

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