Evaluation of biological behavior of odontogenic keratocyst and orthokeratinized odontogenic cyst using picrosirius red stain: A clinicopathological retrospective study

ABSTRACT Background Odontogenic keratocyst (OKC) is an aggressive cyst with neoplastic behavior and unicystic ameloblastoma (UA) is a neoplasm with cyst like behavior. Both odontogenic keratocyst and unicystic ameloblastoma show biologic behavior unlike other lesions in their respective groups. In the present study, the biological behavior of these lesions were assessed by studying the collagen fibers in their walls using picrosirius red (PSR) polarization method. Methods Collagen fibers in 20 cases of OKC and 20 cases of UA were studied histologically by staining sections with picrosirius red dye and examining them by polarizing microscopy. Polarization colors of the collagen fibers of the lesions were recorded according to their width. Results While no differences were seen between the polarization colors of thin fibers (< 0.8 μ) in both odontogenic keratocyst and unicystic ameloblastoma, the polarization colors of thick fibers of odontogenic keratocyst were significantly more greenish-yellow as compared to the unicystic ameloblastoma which were predominantly yellowish-red. Conclusion These findings suggest that odontogenic keratocyst is a more aggressive lesion than unicystic ameloblastoma by means of identifying abnormally packed collagen fibers in odontogenic keratocyst but not in unicystic ameloblastoma. Thus, the nature of collagen fibers as studied by the picrosirius red polarization method may be useful as a diagnostic tool to differentiate between the two lesions.

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The Advent of Studies on Jaw Cysts with Keratinization: A Review of Overlooked Papers on Odontogenic Keratocyst and Orthokeratinized Odontogenic Cyst

Head and Neck Pathology ◽

10.1007/s12105-019-01115-0 ◽

2019 ◽

Vol 14 (3) ◽

pp. 785-791

Author(s):

Fumio Ide ◽

Yumi Ito ◽

Takashi Muramatsu ◽

Yuji Miyazaki ◽

Michiko Nishimura ◽

...

Keyword(s):

Odontogenic Cyst ◽

Odontogenic Keratocyst ◽

Jaw Cysts ◽

Orthokeratinized Odontogenic Cyst

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Odontogenic Keratocyst, Orthokeratinized Odontogenic Cyst and Epidermal Cyst: An Immunohistochemical Study Including Markers of Proliferation, Cytokeratin and Apoptosis Related Factors

International Journal of Oral-Medical Sciences ◽

10.5466/ijoms.2.14 ◽

2004 ◽

Vol 2 (1) ◽

pp. 14-22 ◽

Cited By ~ 7

Author(s):

Yasuyuki Koizumi

Keyword(s):

Immunohistochemical Study ◽

Odontogenic Cyst ◽

Odontogenic Keratocyst ◽

Epidermal Cyst ◽

Related Factors ◽

Orthokeratinized Odontogenic Cyst

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Prevalence and clinicopathological comparison of kerotocystic odontogenic tumor and orthokeratinized odontogenic cyst in South Indian sample population: A retrospective study over 13 years

Journal of Pharmacy And Bioallied Sciences ◽

10.4103/0975-7406.137418 ◽

2014 ◽

Vol 6 (5) ◽

pp. 127 ◽

Cited By ~ 4

Author(s):

Manickam Selvamani ◽

AndamuthuYamuna Devi ◽

PraveenS Basandi ◽

GS Madhushankari

Keyword(s):

Retrospective Study ◽

Odontogenic Cyst ◽

Odontogenic Tumor ◽

Sample Population ◽

Orthokeratinized Odontogenic Cyst ◽

South Indian

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HIF-1α is Overexpressed in Odontogenic Keratocyst Suggesting Activation of HIF-1α and NOTCH1 Signaling Pathways

Cells ◽

10.3390/cells8070731 ◽

2019 ◽

Vol 8 (7) ◽

pp. 731 ◽

Cited By ~ 1

Author(s):

Natacha Malu Miranda da Costa ◽

Caio Tadashi Saab Abe ◽

Geovanni Pereira Mitre ◽

Ricardo Alves Mesquita ◽

Maria Sueli da Silva Kataoka ◽

...

Keyword(s):

Growth Factor ◽

Hypoxia Inducible Factor ◽

Odontogenic Cyst ◽

Odontogenic Keratocyst ◽

Biological Behavior ◽

Heparin Binding ◽

Normal Oral Mucosa ◽

Adam 12 ◽

Epithelial Layers ◽

Related Proteins

Background: The odontogenic keratocyst (OKC) is an odontogenic cyst that shows aggressive and intriguing biological behavior. It is suggested that a hypoxic environment occurs in OKC, which led us to investigate the immunoexpression and location of hypoxia-inducible factor 1-alpha (HIF-1α) and other hypoxia-related proteins. Methods: Twenty cases of OKC were evaluated for the expression of Notch homolog 1 (NOTCH1), HIF-1α, disintegrin and metalloproteinase domain-containing protein 12 (ADAM-12), and heparin-binding epidermal growth factor-like growth factor (HBEGF) by immunohistochemistry and compared to eight control cases of calcifying odontogenic cystic (COC), orthokeratinized odontogenic cyst (OOC), and normal oral mucosa (OM) in basal and parabasal layers. Results: In OKC, all the proteins tested were expressed significantly higher in both basal (except for NOTCH1 and HBEGF in OOC) and suprabasal epithelial layers compared to controls. Looking at the epithelial layers within OKC, we observed an increased NOTCH1 and HIF-1α expression in parabasal layers. Conclusions: These results suggest that hypoxia occurs more intensively in OKC compared to COC, OM, and OOC. Hypoxia appeared to be stronger in parabasal layers as observed by higher HIF-1α expression in upper cells. Overexpression of NOTCH1, ADAM-12, and HBEGF in OKC was observed, which suggests that microenvironmental hypoxia could potentially regulate the expression of hypoxia-related proteins, and consequently, its clinical and biological behavior.

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