scholarly journals Genotypic variations of porphyromonas gingivalis in chronic periodontitis patients with and without diabetes: An in vitro study using arbitrarily primed-polymerase chain reaction and heteroduplex-polymerase chain reaction

2019 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
Siladitya Sen ◽  
KukkamallaMeena Anand
Keyword(s):  
Polymerase Chain Reaction ◽  
Porphyromonas Gingivalis ◽  
Chronic Periodontitis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Identification of Multiple Strains of Porphyromonas gingivalis using Heteroduplex Polymerase Chain Reaction in Varying Severity of Chronic Periodontitis

2018 ◽  
Vol 36 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Mihir Raghavendra Kulkarni ◽  
Kishore G Bhat ◽  
Betsy Sara Thomas ◽  
G.Subraya Bhat ◽  
Raghavendra Dhirendra Kulkarni
Keyword(s):  
Polymerase Chain Reaction ◽  
Porphyromonas Gingivalis ◽  
Chronic Periodontitis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Multiple Strains

scholarly journals Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study

2002 ◽  
Vol 35 (5) ◽  
pp. 487-490 ◽  
Author(s):  
Rozália F. Campos ◽  
Juracy B. Magalhães ◽  
Eliana A.G. Reis ◽  
Mitermayer G. Reis ◽  
Sonia G. Andrade
Keyword(s):  
Polymerase Chain Reaction ◽  
Trypanosoma Cruzi ◽  
In Vitro Study ◽  
High Sensitivity ◽  
Positive Control ◽  
Chain Reaction ◽  
Negative Results ◽  
Polymerase Chain ◽  
Positive Controls

To evaluate the sensitivity of polymerase chain reaction (PCR) to reveal known number of trypomastigote in the blood of mice, three separate experiments were done. First: To eight samples of 500mul of normal mice blood, one aliquot of 1, 2, 3, 4, 5, 10, and 50 trypomastigotes respectively, were added. Second and third: 10 aliquots with 1 and 10 with 2 trypomastigotes were added to samples of 500mul of normal mice blood. Positive control: 500mul of blood containing 100,000 trypomastigotes. For kDNA minicircles amplification by PCR the primers:S35 and S36 were used. PCR revealed products of 330 b.p in the positive controls. When only one sample with the aliquots of 1 or 2 trypomastigotes was examined, results were negative; results were positive with aliquots of 3 to 50 trypomastigotes. In the 2nd and 3rd experiments, 9/10 aliquots with one parasite and 9/10 with 2 trypomastigotes were positive revealing a high sensitivity of this reaction. In conclusion, the presence of one single parasite in 500mul of blood, is enough for a positive PCR. This method could be used as a complement to the various parasitological cure tests in treated mice, when low volumes of blood are individually examined.

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