scholarly journals Tuberculosis cutis orificialis with underlying pulmonary tuberculosis in an immunocompetent man

2018 ◽  
Vol 10 (04) ◽  
pp. 457-459 ◽  
Author(s):  
Biswajit Dey ◽  
Archana Hemant Deshpande ◽  
Sanjeev Hosdurg Bhat ◽  
Ashok Singh
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Pulmonary Tuberculosis ◽  
Buccal Mucosa ◽  
Delayed Diagnosis ◽  
Nucleic Acid Amplification ◽  
Chain Reaction ◽  
Rare Form ◽  
Polymerase Chain ◽  
The Right

ABSTRACTTuberculosis cutis orificialis (TCO) is a rare form of tuberculosis more often secondary to pulmonary tuberculosis. TCO has varied differential diagnoses and thus results in delayed diagnosis and treatment leading to serious consequences. The diagnosis of TCO is confirmed by biopsy and nucleic acid amplification tests in majority of cases. We report a case of TCO with underlying pulmonary tuberculosis in a 50-year-old male, who presented with a painful nonhealing ulcer of the right buccal mucosa. Biopsy and real-time polymerase chain reaction helped in confirming the diagnosis. The patient was treated with antitubercular therapy.

Ribonuclease H-cleavable and recombinase-quenching fluorescent probes for the real-time detection of strand invasion based amplification

2019 ◽  
Vol 11 (43) ◽  
pp. 5568-5576
Author(s):  
Sonja Elf ◽  
Kevin E. Eboigbodin
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Fluorescent Probes ◽  
Nucleic Acid Amplification ◽  
Ribonuclease H ◽  
Chain Reaction ◽  
Amplification Method ◽  
Polymerase Chain ◽  
Strand Invasion

SIBA is an established nucleic acid amplification method that is used as an alternative to polymerase chain reaction (PCR).

scholarly journals La PCR e le sue varianti

2008 ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Polymorphism ◽  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Nucleic Acid Amplification ◽  
Genetic Identification ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Reference Tool

The book "La PCR e le sue varianti" is designed as a reference tool for those whose laboratory activities deal with methods based on nucleic acid amplification. The text provides the theoretical bases of the polymerase chain reaction (PCR) and its variants (e.g. RT-PCR, quantitative PCR, isothermic PCR) in a rapid and concise manner and describes the principal applications used for genetic identification and the study of genetic polymorphism, in the form of a protocol that can be easily consulted by the users.

scholarly journals The discovery of Bungowannah virus – an example of the need for conventional and new technologies

2009 ◽  
Vol 30 (4) ◽  
pp. 138
Author(s):  
Peter D Kirkland
Keyword(s):  
Monoclonal Antibody ◽  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
New Technologies ◽  
Nucleic Acid Amplification ◽  
Chain Reaction ◽  
Agar Gel ◽  
Human Illness ◽  
Polymerase Chain ◽  
Flying Fox

A novel disease in pigs and another new virus ? so where is the flying fox connection? This was one of the first questions that many observers asked from the sidelines. In this instance there was no known connection with flying foxes, no suggestion of human illness but, as the investigation unravelled, a probable cause was identified ? an apparently new virus that had close connections to an important pig pathogen that is exotic to Australia. This virus was, however, so genetically different from its relatives that pan reactive polymerase chain reaction-based assays would not detect it. It was so different antigenically that pan reactive monoclonal antibody panels would react with it. Nevertheless, a combination of simple agar gel immunodiffusion tests for antibodies and sophisticated nucleic acid amplification and sequencing methods proved to be the keys to the recognition of this virus.

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