Degradability and digestibility of full-fat soybeans treated with different sugar and heat combinations

1999 ◽  
Vol 79 (2) ◽  
pp. 213-220 ◽  
Author(s):  
Hélène V. Petit ◽  
Gaëtan F. Tremblay ◽  
Michèle Marcotte ◽  
Richard Audy

Two experiments were conducted to study the effects of different combinations of sugars and heating time and temperature on the ruminal undegradable protein fraction and DM digestibility of full-fat soybeans. In the first experiment, the influence of sugar addition before heating (none, soaked in either a high fructose corn syrup [HFCS] solution, lignosulfonate or molasses), micronization temperatures (100, 110, 120, and 130 °C), and temperature holding times (0, 30, 60, and 120 min) was examined in a 4 × 4 × 4 factorial arrangement of treatments. In the second experiment, the effect of heating in a sugar solution was studied in a 4 × 2 × 4 factorial design with four sugar treatments (none, soaked either in fructose, molasses or a high xylose product), two soaking temperatures (100 and 120 °C), and four soaking times (0, 60, 120, and 240 min). In vitro dry matter digestibility (IVDMD), in vitro ruminal undegradable protein (RUP), and in sacco RUP were measured. In the first experiment, increased temperatures and heating to 120 min resulted in an increased RUP fraction of full-fat soybeans with no effect on DM digestibility, relative to when no sugar or fructose was added. There was a decrease in digestibility with increased temperature when lignosulfonate or molasses was added and with increased heating time when lignosulfonate was added. In the second experiment where soaking in sugar and heating were done simultaneously, lignosulfonate increased the RUP of soybeans without affecting its digestibility but values were numerically lower than when soaking and micronizing were conducted in two different steps as performed in the first experiment. Key words: protein, degradability, digestibility, micronization

2002 ◽  
Vol 82 (2) ◽  
pp. 241-250 ◽  
Author(s):  
Hélène V. Petit ◽  
Gaëtan F. Tremblay ◽  
Eric Tremblay ◽  
Paul Nadeau

Two experiments were conducted to study the effects of processing techniques on in vitro ruminal undegradable protein (RUP), in vitro ruminal biohydrogenation of fatty acids, and in vitro dry matter digestibility (IVDMD) of rolled flaxseed. In the first experiment, the influence of micronization treatment (130°C for 4 h, 140°C for 2 h, 150°C for 1 h, and 160°C for 0.5 h) was examined. The IVDMD significantly decreased when heating at 150°C for 1 h and 160°C for 0.5 h. Degradation rate of crude protein (CP) decreased and RUP concentration increased in parallel with heating temperature. Micronization had little effect on biohydrogenation of fatty acids. In the second experiment, the influence of sugar addition [none, soaked in a high fructose corn syrup (HFCS) or a lignosulfonate solution] before heating, either in an autoclave or a forced-air oven, at two temperatures (100 or 120°C), and two temperature holding times (30 and 60 min) was examined. Increased temperature and heating time decreased degradation rate of CP and increased the RUP fraction of flaxseeds. There was a decre ase in IVDMD with increased temperature when fructose was added. Moist heating is a better option than dry heating, but it has little advantage over not heating to prevent ruminal biohydrogenation of polyunsaturated fatty acids of flaxseeds. Key words: Biohydrogenation, degradability, in vitro, micronization, lignosulfonate


Author(s):  
K. Sridhar ◽  
C. H. Jagadeesh ◽  
Y. Ramana Reddy ◽  
D. Nagalakshmi ◽  
M. Mahender ◽  
...  

APBN1, a variety of hybrid napier was analysed at five stages of harvest (30, 40, 45, 50 and 60 days) for yield, leaf to stem ratio, chemical composition, in vitro dry matter digestibility, in sacco dry matter and protein degradation to find out optimum stage of harvesting. The yield of green fodder, dry matter (DM) and crude protein (CP) (MT/ ha/year) of forage harvested at 30, 40, 45, 50 and 60 days of growth ranged from 274.68 to 387.95, 47.35 to 98.81, 7.44 to 11.45, respectively. Highest (P less than 0.05) CP yield (11.45 MT/ha) and nitrogen free extract content (46.12%) was observed at 45 d of growth. Crude protein (15.72 to 11.34%), ether extract (3.65 to 2.26%) decreased, whereas total ash (10.88 to 13.86%), crude fibre (23.81 to 29.88%) and acid insoluble ash (3.92 to 5.14%) levels increased when forage was allowed to grow from 30 to 60 d. Similarly, NDF, ADF, hemicellulose, cellulose, lignin and silica levels also increased gradually as plant matures. The in vitro dry matter digestibility was maximum (57.87%) at 30 d and minimum (51.40%) at 60 d of growth. Effective dry matter and protein degradability decreased from 55.80 to 43.78 and from 38.30 to 33.17 per cent, respectively as plant matured from 30 to 60 days. The insoluble but degradable protein fraction ‘b’ was highest (77.07%) at 45 d of growth. Based on the biomass yield, leaf to stem ratio, chemical, in vitro and in sacco evaluation, the optimum stage of harvesting for APBN1 forage was found to be at 45 d of growth.


2002 ◽  
Vol 53 (1) ◽  
pp. 7 ◽  
Author(s):  
Tessema Zewdu ◽  
Robert Baars ◽  
Alemu Yami ◽  
Dawit Negassa

In vitro dry matter digestibility (IVDMD) as well as in sacco rumen dry matter degradability (DMD) and nitrogen degradability (ND) studies of Napier grass (Pennisetum purpureum) were carried out using a 3 3 factorial experiment arranged in a randomised complete block design with 3 replications. The treatments were 3 levels of nitrogen (N) fertiliser (0, 46, bacterium Synergistes jonesii is known to degrade these compounds and inoculation of the bacterium into the rumen protects cattle against mimosine and DHP toxicity. We report on the development, production, and storage of an in vitro, fermentor-based, mixed bacterial inoculum, which includes S. jonesii, for administration to cattle. This technique may be applicable to the production and delivery of other live, anaerobic, bacterial inocula for use in animal agriculture.


Author(s):  
Janet H. Woodward ◽  
D. E. Akin

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.


Crop Science ◽  
1986 ◽  
Vol 26 (6) ◽  
pp. 1123-1126 ◽  
Author(s):  
N. J. Ehlke ◽  
M. D. Casler ◽  
P. N. Drolsom ◽  
J. S. Shenk

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.


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