scholarly journals Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

2018 ◽  
Vol 53 (9) ◽  
pp. 1045-1052
Author(s):  
Mateus Merlo Coelho ◽  
Lúcio Carlos Gonçalves ◽  
José Avelino Santos Rodrigues ◽  
Kelly Moura Keller ◽  
Gustavo Vinícius de Souza dos Anjos ◽  
...  

Abstract: The objective of this work was to evaluate the effects of re-ensiling and bacterial inoculation on the quality of corn silage. The experiment was carried out in a 2x2 factorial design with or without inoculant (association of Lactobacillus plantarum and Propionibacterium acidipropionici), and with re-ensiling after 36 hours of aerobic exposure or only ensiling of the whole plant of 'BRS 1055' corn. The fermentative quality, nutritional parameters, dry matter losses, aerobic stability, and microbiological counts of silages were evaluated. Re-ensiling caused an increase of pH and in acetic acid and propionic acid concentrations, as well as in the dry matter (DM), crude protein, neutral detergent fiber, and neutral detergent fiber crude protein contents. Conversely, there was a reduction in the nonfiber carbohydrates concentration and in in vitro dry matter digestibility for the re-ensiled material. All changes were explained by the higher-effluent production and DM loss of re-ensiled material that was subjected to two compactions. Microbiology was not altered by the treatments. The use of inoculant altered ash content, but it did not influence other parameters. In contrast, re-ensiling after 36 hours of aerobic exposure caused a reduction in the nutritive value of corn silage and accentuated the DM losses.


2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Mikael Neumann ◽  
Guilherme Fernando Mattos Leão ◽  
Eloize Jaqueline Askel ◽  
Fabiano Marafon ◽  
Danúbia Nogueira Figueira ◽  
...  

ABSTRACT: Forage conservation in silos depends on the efficiency of silo sealing, among other factors, to minimize aerobic deterioration and consequent qualitative and quantitative losses in silage. Thus, the aim of the present study was to evaluate the effect of two sealing types on dry matter recovery rate, aerobic stability, nutritional composition, and in vitro digestibility of corn silage in bunker silos: 110-µm-thick double-sided polyethylene sealing (conventional sealing) and110-µm-thick double-sided polyethylene sealing superimposed on 111-µm-thick translucent polyethylene sealing (double sealing). There were no significant differences in nutritional composition of silages between conventional and double sealing. However, the double sealing system was more efficient (P<0.05) in maintaining lower silo temperatures (30.48°C vs. 31.18°C), in dry matter recovery (88.79% vs. 85.64%), and increased in vitro neutral-detergent fiber digestibility (33.04% vs. 24.6%), when compared to the conventional version.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 403-404
Author(s):  
Dimas Hand Vidya Paradhipta ◽  
Seong Shin Lee ◽  
Hyuk jun lee ◽  
Young Ho Joo ◽  
In-Hag Choi ◽  
...  

Abstract This study estimated the effects of new inoculants on fermentation quality and rumen digestibility of rye silage with different moisture contents. New inoculants were isolated for producing antifungal substances (Lactobacillus brevis 100D8) and fibrinolytic enzymes (Leuconostoc holzapfelii 5H4), and mixed at 1:1 ratio. Rye forage was harvested at 20 days after heading stage, and chopped into 3–5 cm length. The chopped forage ensiled immediately (high moisture, High) or after 8 h wilting (low moisture, Low) into 10-L mini silo (8kg) with new inoculants at 7.5 × 105 cfu/g (MIX) or commercial inoculant (L. plantarum CMbio) at 8.0 × 105 cfu/g (LPT) for 100 days in quadruplicate. Wilting process did not affect chemical compositions of rye forages, except dry matter (DM) content (P &lt; 0.001; 42.8 vs. 34.7%) was higher in High than in Low. Rye silage in High had lower (P &lt; 0.001) DM with higher (P &lt; 0.05) neutral detergent fiber (NDF), and in vitro digestibilities of DM (IVDMD) and NDF (IVNDFD) than in Low. Additionally, NDF (P &lt; 0.01), hemicellulose (P = 0.005), IVDMD (P = 0.005), and IVNDFD (P = 0.045) of rye silages were higher in MIX than those in LPT. Rye silage had lower (P &lt; 0.05) pH by High or MIX application. Acetate concentration of rye silage was higher (P &lt; 0.01) in MIX than in LPT, but lower (P &lt; 0.001) lactate to acetate ratio. Yeast was lower (P = 0.006) in MIX than in LTP. There were the interaction effects (P &lt; 0.05) between moisture and inoculant on ammonia-N, lactate, propionate, lactic acid bacteria, and aerobic stability of rye silage. Therefore, this study could conclude that new inoculant improved the fermentation quality of rye silage harvested at early dough stage, which showed the beneficial effects on rumen digestibility with high moisture rye forage and on aerobic stability with low moisture.


1988 ◽  
Vol 68 (3) ◽  
pp. 787-799 ◽  
Author(s):  
V. GIRARD ◽  
G. DUPUIS

In view of the large variation found in plant cell wall digestibilities with ruminants, an attempt was made to group 124 feeds into different lignification classes (clusters) on the basis of chemical characteristics. Each feed cluster was described using a structural coefficient [Formula: see text] that related the potentially digestible fiber (PDF, %) to the ratio between lignin and cell wall volume. The optimum number of clusters was determined iteratively by performing a regression of the apparent digestibility of dry matter at maintenance level (DDM1, %) against the PDF and cell soluble (SOL, %) contents of feeds. The [Formula: see text] coefficients varied from 0.05 (grains, N = 13) to 1.85 (corn silage, N = 3) and increased with the maturity of the grasses from 0.88 (legumes, vegetative cool season grasses, N = 26) to 1.33 (mature, cool season grasses, N = 19). Predicted PDF were closely correlated (r > 0.9, P < 0.01) to in vitro cell wall disappearances (IVCWD). Apparently digestible cell wall in four grasses and four legumes increased linearly with 96-h IVCWD and standard error (SE) was similar to the SE of predicted apparent digestible SOL from SOL concentrations. Assuming that similarity between SE could be also observed in larger samples, PDF and SOL were used in summative equations to predict apparent dry matter digestibility. DDM1 discounted for intake (DDM1 – 4, %) was regressed against SOL and PDF concentrations of 87 feeds:[Formula: see text]with ds and df, the true digestibilities of SOL and PDF. Estimates of ds and df were 0.98 and 0.95 for a zero-production (maintenance) level of intake, and 0.91 and 0.79 for an intake level four times maintenance. Since the true digestibility of the PDF component was only 4% – 13% lower than that of the cell soluble component, the concentration of PDF in cell wall was the major determinant in the variation in apparent digestibility of forages. Key words: lignin, neutral detergent fiber, true digestibility, cluster analysis, feeds


2018 ◽  
Vol 39 (5) ◽  
pp. 2071
Author(s):  
João De Assis Farias Filho ◽  
Fabiana Luiza Matielo de Paula ◽  
Adalberto Luiz de Paula ◽  
Wagner Paris ◽  
Fabrício Ghinzelli ◽  
...  

The objective of this work was to evaluate the effects of irrigation and nitrogen fertilization on the bromatological quality, forage production, and botanical and structural composition of Tifton 85 (Cynodon sp.) pastures overseeded with black oat (Avena strigosa). Four treatments were evaluated in a 2 × 2 factorial scheme (irrigated and non-irrigated × fertilized and non-fertilized), with three replicates, in a completely randomized design. In irrigated paddocks, the irrigation system was activated when the soil matric potential reached a value equal to, or higher than, 10 kPa and 135 kg N ha-1 was applied to fertilized paddocks, divided into four applications. The forage mass pre- and post-grazing, total forage production, and the botanical, structural, and bromatological composition of the pastures were evaluated. No interaction was observed between the irrigation and nitrogen fertilization factors for any of the variables and no significant differences were observed in forage mass between pre- and post-grazing or in Tifton leaf and stem percentages. Nitrogen fertilization had a significant effect on forage production, which was 2626.41 kg dry matter (DM) ha-1 higher in fertilized pastures than in unfertilized pastures. In addition, fertilization resulted in a lower percentage of dead material (6.66%), higher percentage of oat leaves (30.84%), higher leaf:stem ratio (1:45), higher crude protein content (24.13%), lower levels of neutral detergent fiber (NDF) (64.57%) and acid detergent fiber (ADF) (32.86%), and higher in vitro dry matter digestibility (73.01%) than in unfertilized pastures. The use of irrigation did not influence total forage production, however, it resulted in pastures with lower NDF (65.97%) and ADF (33.54%), and higher in vitro dry matter digestibility (73.48%) than unirrigated pastures. Nitrogen fertilization produced improvements in pasture structure, associated with higher dry matter yield and bromatological quality, while irrigation only produced pastures with lower fiber content and greater digestibility.


2019 ◽  
Vol 40 (5) ◽  
pp. 2045 ◽  
Author(s):  
Flávio Pinto Monção ◽  
Marco Aurélio Moraes Soares Costa ◽  
João Paulo Sampaio Rigueria ◽  
Marielly Maria Almeida Moura ◽  
Vicente Ribeiro Rocha Júnior ◽  
...  

The objective was to evaluate the productivity, chemical composition, ruminal degradability of dry matter and digestibility of BRS capiaçu grass (Pennisetum purpureum Schum.) managed at five regrowth ages. A completely randomized design with ten replications was used in the BRS capiaçu elephant grass subjected to five cutting intervals (30, 60, 90, 120 and 150 days) in the summer, making a total of 50 plots with a useful area of 4 x 2 m. There were daily increases in the dry matter production in the order of 382 kg ha-1, with 49,859 kg ha-1 being produced at 150 regrowth days. The dry matter content and organic matter linearly increased (P < 0.01) at different regrowth ages. The crude protein content, in vitro dry matter digestibility and in vitro digestibility of neutral detergent fiber linearly reduced (P < 0.01) 0.037%, 0.196% and 0.256% per day, respectively. Potential degradability of dry matter decreased from 68.9% at 30 days to 44.7% at 150 regrowth days (0.194 percentage units per day). The rate of degradation of fraction B 'c' was not modified (P = 0.94), averaging 1.46% hour-1. In the cultivation of BRS capiaçu elephant grass in the summer season, in the northern region of Minas Gerais, the age for harvesting between 90 and 120 days of regrowth is recommended.


2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Zhihao Dong ◽  
Junfeng Li ◽  
Lei Chen ◽  
Siran Wang ◽  
Tao Shao

ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased (P<0.05) the lactic acid concentration and lowered (P<0.05) the pH when compared with control silage. The best fermentation properties were observed in L+M silage, as indicated by the dominance of lactic acid over ethanol in fermentation products. The M and L+M silages exhibited higher (P<0.05) dry matter, and M silage showed higher residual water-soluble carbohydrates than the control. Ensiling increased (P<0.05) the in vitro dry matter, neutral detergent fiber and acid detergent fiber degradability of TBL. Among the silages, M silage had the highest levels of dry matter, neutral detergent fiber and acid detergent fiber degradability. The obtained results suggested that application of lactic acid bacteria together with 3% molasses could be an effective strategy to prevent the occurrence of ethanol fermentation and improve fermentation quality of TBL silage; addition of fermentable sugars to TBL improves nutrient availability to ruminants.


2000 ◽  
Vol 80 (2) ◽  
pp. 315-325 ◽  
Author(s):  
G. F. Tremblay ◽  
R. Michaud ◽  
G. Bélanger ◽  
K. B. McRae ◽  
H. V. Petit

The quality of alfalfa would be greatly improved by an increase in its ruminal undegradable protein (RUP) concentration. Protein degradation rate (PDR), in vitro dry matter digestibility (IVDMD), leaf weight ratio (LWR), dry matter yield (DMY), total nitrogen (TN), in vitro RUP (expressed on both TN, RUP-TN, and dry matter basis, RUP-DM), acid detergent fiber (ADF), and neutral detergent fiber (NDF) concentrations were determined in 27 alfalfa cultivars. Cultivars were seeded in triplicate on 2 consecutive years and evaluated during the 2 subsequent production years with two harvests per year. Protein degradation rate and RUP-TN were determined using a ruminal inhibitor in vitro system. Data were averaged for spring growth, summer regrowth, and both harvests across 2 production years. Each of the three data sets was analyzed by ANOVA followed by a principal component analysis (PCA) on the ANOVA means. For the four-harvest data, cultivar differences were highly significant (P < 0.001) for all variates except for PDR (P = 0.07) and RUP-TN concentration (P = 0.10). The first PCA axis was largely defined positively by RUP-DM, IVDMD, TN, LWR, and RUP-TN, but negatively with ADF, NDF, PDR, and DMY. The second PCA axis defined a contrast between PDR versus RUP-TN, DMY, ADF, and NDF. Five cultivars were distinctive with high or low PCA scores in all three PCA. Rangelander and Heinrichs, along with Ultra, had low PDR; the first two cultivars had low DMY whereas Ultra was a medium-yielding cultivar. In contrast, Algonquin and Oneida VR had high PDR and medium DMY. While the first principal component (PC) indicated a general trend that low PDR and high RUP were associated with low-yielding cultivars, the second PC identified specific cultivars with both low PDR and high DMY. Therefore, selection for low PDR and high DMY is feasible. Key words: ruminal protein escape, dry matter digestibility, alfalfa


2017 ◽  
Vol 38 (3) ◽  
pp. 1483
Author(s):  
Loreno Egidio Taffarel ◽  
Paulo Sérgio Rabello de Oliveira ◽  
Euclides Reuter de Oliveira ◽  
Elaine Barbosa Muniz ◽  
Eduardo Eustáquio Mesquita ◽  
...  

Morphological characteristics, dry matter production, and nutritional values of winter forage and grains were evaluated. This study was conducted from April 24, 2012 to November 7, 2013 in the Western Paraná State University (UNIOESTE), Marechal Cândido Rondon, Brazil. Pastures under one grazing and non-grazing conditions were evaluated under 120 kg N ha-1 fertilization split into two 60 kg N ha-1 treatments. Two pastures received 40 kg N ha-1 three times. IPR 126 oat, BRS Tarumã wheat, and IPR 111 triticale were the test crops. Topdressing with 40 or 60 kg N ha-1 did not change morphological characteristics until 60 d after sowing. Pastures under non-grazing that received 120 kg N ha-1 treatments were taller than the controls, whereas those under grazing that received 80 or 120 kg N ha-1 presented with higher leaf production than did the controls. Total average dry matter (DM) production in 2012 and 2013 was, respectively, 5,275 kg ha-1 and 6,270 kg ha-1 for oat, 3,166 kg ha-1 and 7,423 kg ha-1 for wheat, and 4,552 kg ha-1 and 7,603 kg ha-1 for triticale. Split N fertilization did not cause differences in the levels of crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) in the forage. Nevertheless, increases in in vitro dry matter digestibility (IVDMD) were observed in oat and wheat receiving 60 kg N ha-1 during the first graze. IVDMD did not change in oat, wheat, and triticale forages receiving 80 or 120 kg N ha-1 during the second graze. Grazing did not affect the nutritional values of wheat and triticale grains, but reduced those of oat. Therefore, the results of the present study suggest that grazing lengthens the crop cycles, and so allow the staggered sowing of summer crops.


2020 ◽  
Vol 41 (1) ◽  
pp. 237
Author(s):  
João Pedro Velho ◽  
Pagiel Bernardi Zardin ◽  
Clóves Cabreira Jobim ◽  
Ione Maria Pereira Haygert-Velho ◽  
Dileta Regina Moro Alessio ◽  
...  

The objective of this study was to conduct a meta-analysis of scientific publications from January 1994 to December 2014, to evaluate the chemical composition of corn plants, green forage, and silages of three different types of maize hybrids (simple, double, and triple) used in experimental conditions in Brazil. The sub-bases Corn Cultivation and Green Fodder were composed of 49 experiments, consisting of 481 treatments, and the sub-base Silage consisted of 24 studies composed of 163 treatments, involving 478 silos. In the Corn Cultivation sub-base, the variables of spacing, plant height, grain yield, organic matter content, and total carbohydrates of maize silage differed (P < 0.05) according to the hybrid type. The number of corn plants per hectare, number of spikes per plant, and the levels of neutral detergent fiber, acid detergent fiber, hemicellulose, and lignin did not differ (P > 0.05) according to the hybrid type. In the Green Forage sub-base (ensilage), the main difference (P < 0.05) was regarding the in vitro dry matter digestibility, whereby the triple hybrids (59.38%) had lower values than the simple (73.05%) and double (72.74%) hybrids. In the Silage sub-base, the variables of organic matter, total carbohydrates, cellulose, in vitro dry matter digestibility, and pH differed (P < 0.05) according to the hybrid type. While the variables dry matter, non-fibrous carbohydrates, neutral detergent fiber, acid detergent fiber, hemicellulose, lignin, total digestible nutrients, ethereal extract, crude protein, and ammonium nitrogen did not differ (P > 0.05) according to the hybrid type. The variability in the determination of parameters, such as dry matter yield, dry matter content, and non-fibrous carbohydrates of corn silage, and the non-determination of the starch content demonstrated that the chemical compositions of Brazilian corn silage have not yet been fully clarified. The types of maize hybrids (simple, double, and/or triple) mainly differ in terms of grain production. In many situations, the silage processes are not conducted in an ideal way, and the differences as a function of the maize plants (type of hybrid) are minimized due to losses during the ensiling process.


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