EFFECT OF A SUSTAINED-RELEASE FORM OF SOMATOTROPIN ON THE PROFILE OF MILK PROTEINS AND FATTY ACIDS DURING A FULL LACTATION

1990 ◽  
Vol 70 (3) ◽  
pp. 811-819 ◽  
Author(s):  
M. LÉONARD ◽  
H. T. HUNG ◽  
G. ROBITAILLE ◽  
E. BLOCK

Twenty-three Holstein cows received one injection of recombinant bovine somatotropin (bST) every 28 d at one of four doses: 0, 320, 640, or 960 mg 28 d−1. All injections started in early lactation and continued until the cows were dried-off. Composites of AM and PM milkings were analyzed for fatty acids by gas-liquid chromatography, for percent milk protein by infrared analysis and for casein by electrophoresis. For the 0, 320, 640, 960 mg cow−1 28 d−1 bST treatment groups, average ± SE milk protein percents for the entire lactation were 3.24 ± 0.08%, 3.25 ± 0.07%, 3.31 ± 0.08% and 3.24 ± 0.07%, respectively (P > 0.05); average milk caseins were 2.59 ± 0.07%, 2.61 ± 0.06%, 2.60 ± 0.07% and 2.57 ± 0.06%, respectively (P > 0.05), and average whey proteins were 0.65 ± 0.03%, 0.64 ± 0.03%, 0.71 ± 0.03% and 0.67 ± 0.03%, respectively (P > 0.05). No effect of bST was observed on milk fatty acids and protein at any dose or stage of lactation. Concentrations of αs-, β- and K-casein were not affected by bST treatment (P > 0.05). Fat percentages were 3.78 ± 0.21%, 3.91 ± 0.19%, 3.76 ± 0.19% and 4.02 ± 0.16% for the 0, 320, 640 and 960 mg 28 d−1 treatment (P > 0.05). Milk fatty acids were not affected by bST treatment (P > 0.05). Short chain fatty acids and medium chain fatty acids were decreased (P < 0.05) with progressing lactation while long chain fatty acids were increased in late lactation (P < 0.05). Key words: Somatotropin, composition of milk, caseins, milk fatty acids

1976 ◽  
Vol 4 (6) ◽  
pp. 492-502
Author(s):  
C W Moss ◽  
S B Dees

The cellular fatty acid composition of 112 reference strains and clinical isolates of Pseudomonas species was determined by gas-liquid chromatography (GLC). The presence and relative amounts of cyclopropane, hydroxy, and branched-chain fatty acids were distinguishing features of these strains. Determination of short-chain fatty acids extracted from spent growth media provided an additional means for identifying some strains. Our results show that clinical isolates of pseudomonads can be divided into eight distinct GLC groups. The procedures were especially useful for distinguishing glucose-nonoxidizing pseudomonads, which are difficult to identify by conventional criteria. Since the GLC procedures are simple, rapid, and highly reproducible, they are useful in diagnostic laboratories that process large numbers of cultures. Coupled with selected conventional tests, the analysis of short-chain and cellular fatty acids can be very useful for rapid screening of clinical isolates of Pseudomonas species.


1957 ◽  
Vol 24 (3) ◽  
pp. 366-371 ◽  
Author(s):  
J. C. Hawke

1. Despite the presence of appreciable quantities of steam volatile fatty acids as glycerides in butterfat, no short-chain fatty acids with odd carbon number have been detected by gas-liquid chromatography of the acids.2. Mild oxidation of butterfat for short periods of time produces steam volatile fatty acids with odd carbon number. A large proportion of these acids was formic, with smaller proportions of propionic, valeric and nonanoic acids.3. The relative ease with which short-chain fatty acids are produced on oxidation of fats, points to the danger of contaminating naturally occurring fatty acids with extraneous acids.4. A total analysis of the fatty acids of New Zealand butterfat by gas-liquid chromatography compared well with similar analyses carried out by previous workers by means of esterfractionation analysis.


2018 ◽  
Vol 67 (4) ◽  
pp. 243
Author(s):  
I. SKOUFOS ◽  
A. TZORA ◽  
A. KARAMOUTSIOS ◽  
G. TSANGARIS ◽  
I. GIANNENAS ◽  
...  

The aim of this study was to assess the chemical and microbiological characteristics of goat milk from indigenous Greek breeds, domestic (Capra prisca) and Skopelos. Εach breed’s milk yield was recorded three times during lactation (early, mid and late stage of lactation) for two consecutive years. No significant differences were observed for fat, protein, lactose, casein and solids-non-fat contents of milk from both breeds. The dominant fatty acids (FA) in milk for both breeds were: palmitic (C16:0) and oleic (C18:1). Other abundant FA was stearic (C18:0), myristic (C14:0), capric (C10:0) and lauric (C12:0). The conjugated linoleic acid (CLA) content was similar in both breeds. Linolenic acid content in milk of Capra prisca breed was higher than in Skopelos.A high number of minor short chain fatty acids (SCFA), medium chain fatty acids (MCFA) and long chain fatty acids (LCFA) was also observed in milk fat. SCFA’s increased in mid lactation in goats of Capra prisca breed, while no significant differences were noted during early, mid or late lactation period in medium or long chain FA. In Skopelos breed, SCFA’s increased gradually from early to late lactation, while LCFAs followed a descending order. Enumeration of bacteria presented similar numbers of total viable count in milk of both breeds and higher number of Psychrotrophic ones in milk from Skopelos goats to domestic. Somatic cell counts (SCC) values were significant lower in Skopelos breed compared to Capra prisca. Caprine milk can be an important sourceof health promoting substances and deserves further investigation with specific investigation on milk of indigenous local breeds.


1994 ◽  
Vol 92 (4) ◽  
pp. 629-635 ◽  
Author(s):  
Mercedes Gallardo ◽  
Paloma Munoz De Rueda ◽  
Angel Jesus Matilla ◽  
Isabel Maria Sanchez-Calle

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