An attempt at sperm-mediated gene transfer in mice and chickens

1991 ◽  
Vol 71 (2) ◽  
pp. 287-291 ◽  
Author(s):  
J. S. Gavora ◽  
B. Benkel ◽  
H. Sasada ◽  
W. J. Cantwell ◽  
P. Fiser ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Gene Transfer ◽  
Transgenic Mice ◽  
Key Words ◽  
Plasmid Dna ◽  
Dna Transfer ◽  
Laboratory Mice ◽  
Vitro Fertilization ◽  
Bacterial Plasmid

Experiments were carried out to transform laboratory mice and domestic chickens by use of sperm incubated with bacterial plasmid DNA. Following demonstration of "uptake" of such DNA by sperm of both species, attempts were made to replicate a previously published procedure (Lavitrano et al. 1989, Cell 57: 717–723) for producing transgenic mice through in vitro fertilization (IVF). Also, female mice and hens were inseminated (AI) with sperm which had been incubated in a DNA solution. Such incubation did not influence the fertility or hatchability of the hens' eggs. However, no transformed progeny were detected among 45 mice produced by IVF or among 69 mice and 470 chickens produced by AI. Key words: Sperm-mediated DNA transfer, mice, chickens

Lambs produced from cryopreserved sheep embryos derived by in vitro fertilization of aspirated oocytes

1996 ◽  
Vol 76 (3) ◽  
pp. 465-467 ◽  
Author(s):  
N. Songsasen ◽  
A. Martino ◽  
S. P. Leibo ◽  
S. Walmsley ◽  
J. W. Pollard ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Ethylene Glycol ◽  
Key Words ◽  
In Vitro Maturation ◽  
Oocyte Cryopreservation ◽  
Vitro Fertilization

A total of 104 oocytes were aspirated from 11 mature ewes and 4 prepubertal lambs. After in vitro maturation, fertilization and culture, 11 of the 104 oocytes developed into blastocysts. These were cryopreserved in 1.5 M ethylene glycol. After being thawed, all were transferred into four recipients, two of which became pregnant and delivered a normal lamb. Key words: Oocyte, cryopreservation, in vitro maturation and fertilization, sheep, embryo

scholarly journals Efficiency of Sperm-Mediated Gene Transfer in the Ovine by Laparoscopic Insemination, In Vitro Fertilization and ICSI

2011 ◽  
Vol 57 (2) ◽  
pp. 188-196 ◽  
Author(s):  
Federico PEREYRA-BONNET ◽  
Alejandro GIBBONS ◽  
Marcela CUETO ◽  
Pablo SIPOWICZ ◽  
Rafael FERNÁNDEZ-MARTÍN ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Gene Transfer ◽  
Vitro Fertilization

scholarly journals Fertility comparison between wild type and transgenic mice by in vitro fertilization

2009 ◽  
Vol 19 (4) ◽  
pp. 587-594 ◽  
Author(s):  
Kuzhalini Vasudevan ◽  
James Raber ◽  
Jorge Sztein
Keyword(s):  
In Vitro Fertilization ◽  
Transgenic Mice ◽  
Wild Type ◽  
Vitro Fertilization

scholarly journals Effect of Zona Incision by Piezo-Micromanipulator (ZIP) on In Vitro Fertilization in 21 Transgenic Mice Lines

2009 ◽  
Vol 58 (4) ◽  
pp. 415-419 ◽  
Author(s):  
Yosuke KAWASE ◽  
Takanori TACHIBE ◽  
Toshio HANI ◽  
Hiromi TATEISHI ◽  
Kou-ichi JISHAGE ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Transgenic Mice ◽  
Vitro Fertilization

Sperm-mediated gene transfer–treated spermatozoa maintain good quality parameters and in vitro fertilization ability in swine

2009 ◽  
Vol 72 (9) ◽  
pp. 1163-1170 ◽  
Author(s):  
M.L. Bacci ◽  
A. Zannoni ◽  
M. De Cecco ◽  
P. Fantinati ◽  
C. Bernardini ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Gene Transfer ◽  
Quality Parameters ◽  
Vitro Fertilization

scholarly journals In vivo gene transfer using cetylated polyethylenimine.

2004 ◽  
Vol 51 (3) ◽  
pp. 693-702 ◽  
Author(s):  
Aleksander Sochanik ◽  
Tomasz Cichoń ◽  
Monika Makselon ◽  
Małgorzata Strózyk ◽  
Ryszard Smolarczyk ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Plasmid Dna ◽  
Dna Degradation ◽  
Dna Transfer ◽  
Luciferase Expression ◽  
Luciferase Gene ◽  
Amine Groups ◽  
In Vivo Gene Transfer

This report describes gene transfer in vitro as well as in vivo using cetylated low-molecular mass (600 Da) polyethylenimine (28% of amine groups substituted with cetyl moieties), termed CT-PEI. This compound is hydrophobic and has to be incorporated into liposomes in order to be suitable for gene transfer studies. Serum-induced plasmid DNA degradation assay demonstrated that CT-PEI-containing liposomal carriers could protect complexed DNA (probably via condensation). In vitro luciferase gene expression achieved using medium supplemented with 10% serum was comparable to that achieved in serum-reduced medium and was highest for CT-PEI/cholesterol liposomes, followed by CT-PEI/dioleoylphosphatidylcholine liposomes and PEI 600 Da (uncetylated) carrier. In vivo systemic transfer into mice was most efficient when liposome formulations contained CT-PEI and cholesterol. Higher luciferase expression was then observed in lungs than in liver. liposomes containing cetylated polyethylenimine and cholesterol are a suitable vehicle for investigating systemic plasmid DNA transfer into lungs.

scholarly journals THE QUALITY OF EMBRYOS RESULTED FROM IN VITRO FERTILIZATION BY USING FROZEN SEMEN THAWED IN DIFFERENT TEMPERATURES

2017 ◽  
Vol 3 (1) ◽  
pp. 23
Author(s):  
Rakhmi Yasri ◽  
Ristika Handarini ◽  
Muhammad Imron
Keyword(s):  
In Vitro Fertilization ◽  
Key Words ◽  
Embryo Quality ◽  
Fertilization Rate ◽  
Test Results ◽  
Vitro Fertilization ◽  
Frozen Semen ◽  
Different Temperatures

In vitro fertilization technology in cows is an effort done to utilize ovary waste from cows slughtered in abbatoir. This study was aimed at assessing the qualiy of embryos resulted from in vitro fertilization by using frozen semen thawed in different temperatures. In order to get qualty semen, standardized thawing method is required. It was expected from this study that an optimum thawing temperature for frozen semen was determined to obtain quality transferable embryos. Three treatments consisting of thawing with water 37°C for 30 second (T1), thawing with water 25°C for 30 second (T2), and thawing with water 10°C for 30 second (T3). Data were subjected to an an anlysis of variance (Anova) and a Duncan test. Results showed that oocytes fertilized with frozen semen thawed at 37°C and 10°C had higher fertilization rate and excellent-grade embryos (P<0.05) than did the ones fertilized with frozen semen thawed at 25°C. However, no different effect of thawing temperatures was found on transferable and degenerated embryos (P>0.05). It was concluded that embryos fertilized with Brahman frozen semen in thawed at 37°C had the highest number of embryos (49.66±2.88) and excellent-grade embryos (22.00±4.35). Key words: Embryo quality, In vitro fertilization, frozen semen thawing, Brahman bull.

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