A MODIFIED BEAN ROOT INITIATION TEST FOR AUXINS AND INHIBITORS

1967 ◽  
Vol 47 (3) ◽  
pp. 263-268 ◽  
Author(s):  
P. A. Poapst ◽  
A. B. Durkee ◽  
S. H. Nelson
Keyword(s):  
Cotyledonary Node ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Growth Inhibitors ◽  
Bean Root

Removal of tissues above the cotyledonary node from leafy stem cuttings of Contender beans, after imbibition and prior to potting in root initiation tests, increased the sensitivity to growth inhibitors, and reduced the variation in control plants. Response to small amounts of auxin increased slightly and declined for larger amounts.

scholarly journals SHOOT POSITION, IBA AND AGROBACTERIUM RHIZOGENES INFLUENCE PROPAGATION OF HAZELNUT, CORYLUS AVELLANA

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1150g-1150
Author(s):  
William M. Proebsting ◽  
Nahla V. Bassil ◽  
David A. Lightfoot
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Corylus Avellana ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Influence Propagation ◽  
Rooting Of Cuttings ◽  
Shoot Position

Propagation of Corylus avellana stem cuttings may be limited by either root initiation or bud abscission. We divided juvenile shoots of 3 varieties growing in layering beds in mid-July into 4 or 5 3-node cuttings with leaves at the upper two nodes, except that terminal cuttings had one expanded leaf. Cuttings were treated with 5 mM IBA in 50% EtOH, a mixture of A. rhizogenes strains A7 + 22 or left untreated. IBA and bacteria stimulated rooting of cuttings from all shoot positions. Rooting of the terminal cuttings (<50%) was less than that of the sub-terminal cuttings (>80%). Bud retention was <50% on terminal cuttings, nearly 100% on sub-terminal cuttings. Using juvenile stock plants of various varieties, sub-terminal cuttings treated with Agrobacterium or 5 mM IBA may yield 70-90% cuttings with both roots and buds, Agravitropic roots, characteristic of genetic transformation, were observed on Agrobacterium-treated cuttings. Dot blots probed for TL-DNA were negative, however.

Callus Formation and Root Initiation in Stem Cuttings of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco)

1972 ◽  
Vol 2 (2) ◽  
pp. 121-134 ◽  
Author(s):  
J. C. Heaman ◽  
J. N. Owens
Keyword(s):  
Callus Formation ◽  
Douglas Fir ◽  
Vascular Cambium ◽  
Complex Pattern ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Weekly Basis ◽  
Vascular Tissues ◽  
Rooting Response

Changes taking place at the base of stem cuttings of Douglas-fir during callus formation and root initiation are described. Material was taken from nine sources, including trees of different ages and with different histories of rooting response. Gross morphology and anatomy of the cutting bases are described using serial longitudinal sections of samples taken on a weekly basis over a 4-month period. The basal callus proliferated from the lowest cells of the vascular cambium and a complex pattern of differentiating vascular tissues arose within the callus mass. The root primordia arose in this callus in association with the differentiating phloem and wound cambium. Variation in callus formation and root initiation is described both within and between clones.

scholarly journals Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry

2021 ◽  
Vol 8 (5) ◽  
pp. 147-150
Author(s):  
Alam Khan Samim ◽  
B. S. Shivakumar ◽  
Ganapathi M.
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Growth Regulators ◽  
Biochemical Parameters ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Fresh Weight ◽  
Total Chlorophyll Content

An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.

scholarly journals INFLUENCES OF SUBSTRATES ON ROOTING CLEMATIS SOCIALIS STEM CUTTINGS

HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 497D-497 ◽  
Author(s):  
C.N. Johnson ◽  
D.J. Eakes ◽  
A. N. Wright ◽  
J.L Sibley ◽  
L.L. Bruner
Keyword(s):  
Particle Size ◽  
Endangered Species ◽  
Root Growth ◽  
Genetic Material ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Root Number ◽  
Growth Data ◽  
Growth And Survival ◽  
Root Quality

Clematis socialis Kral, commonly known as the Alabama Leatherflower, is an endangered species with only six known populations in northeast Alabama and northwest Georgia. Cutting propagation of the species will aid in establishing additional self-sustaining populations and provide genetic material for future hybridization and genetic preservation. Such research would also benefit growers, especially native nurseries, who wish to produce this species commercially for its ornamental value. Several experiments were performed to determine the effects of four non-amended substrates on root initiation, root growth, and survival of C. socialis stem cuttings. The four substrates tested included sand, perlite, vermiculite, and a 1 peat (P): 1 pine bark (PB): 1 sand (S) mix (by volume). Some of the best results in the preliminary experiments in 2000 were observed when 2 to 3 node cuttings kept under shade and treated with higher IBA/NAA concentrations were used. In 2004, there was a correlation between root growth and cutting survival and particle size of the substrates. Cuttings rooted in the finer-particle substrates sand and vermiculite had higher cutting survival, root growth, root number, and root quality than those rooted in perlite and the 1 P: 1 PB: 1 S mix. Sand, perlite and vermiculite consistently outperformed the 1 P: 1 PB: 1 S mix which had some of the lowest growth data means. Sand was among the highest performing substrates in all years and it is the most inexpensive and readily available making it the most logical substrate for rooting C. socialis stem cuttings.

scholarly journals Herbicide Use During Propagation Affects Root Initiation and Development

1993 ◽  
Vol 11 (4) ◽  
pp. 157-159
Author(s):  
Charles H. Gilliam ◽  
D. Joseph Eakes ◽  
John W. Olive
Keyword(s):  
Root Initiation ◽  
Stem Cuttings ◽  
Herbicide Application ◽  
Gardenia Jasminoides ◽  
Quality Ratings ◽  
Herbicide Treatments ◽  
Herbicide Use ◽  
Root Quality

Abstract Two studies were conducted to evaluate the influence of herbicides on root initiation and development from stem cuttings of three woody landscape species. In the first experiment, Rhododendron obtusum ‘Hino Crimson’ rooting percentage was suppressed with Rout 3G (oxyfluorfen + oryzalin). Both root quality ratings and root lengths were lower for stem cuttings when herbicides were applied compared to untreated controls. Rooting percentages of Rhododendron × ‘Trouper’ and Gardenia jasminoides ‘August Beauty’ were not affected by herbicide application. Root quality ratings and root lengths were generally lower with Rout 3G, OH-2 3G (oxyfluorfen + pendimethlin), Snapshot 2.5TG (trifluralin + isoxaben), and Southern Weed Grass Control 2.68G (pendimethlin) when compared to untreated controls. In the second experiment, depth of sticking Gardenia jasminoides ‘August Beauty’ cuttings and herbicide treatments were evaluated. Cuttings in Rout 3G and Snapshot 2.5 TG treatments had improved root quality ratings when stuck to a depth of 2.5 cm (1.0 in) compared to 1.3 cm (0.5 in); however, all other herbicide treatments had similar root quality ratings to the untreated controls, regardless of depth of sticking.

scholarly journals EFFECT OF SUBSTRATES ON ROOTING OF STEM CUTTINGS OF THE ENDANGERED SPECIES Clematis socialis

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 875d-875
Author(s):  
C. N. Johnson ◽  
D. J. Eakes ◽  
L. L. Bruner ◽  
A. N. Wright ◽  
J. L Sibley
Keyword(s):  
Endangered Species ◽  
Root Growth ◽  
Environmental Conditions ◽  
Root Length ◽  
Genetic Material ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Root Number ◽  
Cutting Propagation ◽  
Root Quality

Clematis socialis Kral, also known as the Alabama Leatherflower, is an endangered species with only six known populations in northeast Alabama and northwest Georgia. Cutting propagation of the species would be beneficial for establishing additional self-sustaining populations and providing genetic material for future hybridization. A study conducted in 2000 and 2004 determined the effects of four nonamended substrates on root initiation and growth, as well as survival of C. socialis stem cuttings. Of the four substrates tested, including sand, perlite, vermiculite, and 1:1:1 (by volume) peat (P): pine bark (PB): sand (S), cutting survival was highest in sand in both 2000 and 2004. In 2000, sand also produced the longest roots and highest root quality. Vermiculite produced the longest and most roots and highest root quality in the 2004 study. In 2004, cuttings rooted in fine-particled substrates, such as sand and vermiculite, had higher cutting survival, root growth, root number, and root quality than those rooted in perlite and 1:1:1 (by volume) P:PB:S. The 1:1:1 P:PB:S substrate produced the lowest averages for all data collected in both the 2000 and 2004 studies. Sand was among the two highest performing media in both years, regardless of differences in IBA concentration, misting times, and environmental conditions, making it the overall best substrate for rooting C. socialis stem cuttings. Increasing the concentration of IBA in the rooting solution, providing a cooler environment, and decreasing the number and duration of misting cycles the cuttings received increased cutting survival, root length, root number, and root quality for all four substrates from 2000 to 2004.

scholarly journals Change in Tissue Mineral Elemental Concentration during Root Initiation and Development of Poinsettia Cuttings

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 617-619 ◽  
Author(s):  
Sven E. Svenson ◽  
Fred T. Davies
Keyword(s):  
Adventitious Roots ◽  
Elemental Concentration ◽  
Euphorbia Pulcherrima ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Root Primordia ◽  
Foliar N ◽  
Element Concentrations ◽  
Propagation Medium

Variation in tissue elemental concentration in apical stem cuttings of `Lilo' and `V-10 Amy' poinsettia (Euphorbia pulcherrima Willd. ex. Klotzch) were studied during the initiation and development of adventitious roots. Changes in selected macro- and micro-element concentrations coincided with root initiation (i.e., Fe, Cu, and Mo accumulated in the basal portions of stem cuttings during early root initiation before root primordia elongation); P, K, Ca, and Mg concentrations declined. During root primordia elongation and root emergence, Fe, Cu, and Mo and Mg, Mn, B, and Zn concentrations continued to increase at the cutting bases, but P and K concentrations remained low compared to when cuttings were initially inserted in the propagation medium. When all cutting of both cultivars had rooted, foliar N, Fe, and Mo concentrations declined, but Cu increased compared to when cuttings were initially propagated.

scholarly journals Rooting of healthy and CVC-affected 'Valência' sweet orange stem cuttings, through the use of plant regulators

2006 ◽  
Vol 49 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Gustavo Habermann ◽  
Rita de Cássia Félix Alvarez ◽  
Júnior Cesar Modesto ◽  
Andréa Maria Teixeira Fortes ◽  
João Domingos Rodrigues ◽  
...  
Keyword(s):  
Plant Hormone ◽  
Sweet Orange ◽  
Xylella Fastidiosa ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Acid Biosynthesis ◽  
Rooted Cuttings ◽  
Hormone Imbalance ◽  
Gibberellic Acid Biosynthesis ◽  
Rooting Capacity

Citrus variegated chlorosis (CVC) is a disease caused by Xylella fastidiosa. Using different concentrations of plant regulators, such as auxins (indole-3-butyric acid) and gibberellic acid biosynthesis-inhibitor (paclobutrazol), physiological rooting capacity of healthy and CVC-affected stem cuttings were evaluated in order to investigate the importance of plant hormone imbalance and xylem occlusion in plants with CVC. The percentages of dead, alive and rooted cuttings, cuttings with callus and mean number of roots per cuttings did not show statistical differences in response to the distinct concentrations of synthetic plant regulators. There were differences only between healthy and CVC-affected cuttings. This showed the importance of xylem occlusion and diffusive disturbances in diseased plants, in relation to root initiation capacity and hormonal translocation in the plant tissue.

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