AN ANATOMICAL STUDY OF ROOT INITIATION IN STEM CUTTINGS OF HYBRID LARCH

Removal of tissues above the cotyledonary node from leafy stem cuttings of Contender beans, after imbibition and prior to potting in root initiation tests, increased the sensitivity to growth inhibitors, and reduced the variation in control plants. Response to small amounts of auxin increased slightly and declined for larger amounts.

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SHOOT POSITION, IBA AND AGROBACTERIUM RHIZOGENES INFLUENCE PROPAGATION OF HAZELNUT, CORYLUS AVELLANA

HortScience ◽

10.21273/hortsci.25.9.1150g ◽

1990 ◽

Vol 25 (9) ◽

pp. 1150g-1150

Author(s):

William M. Proebsting ◽

Nahla V. Bassil ◽

David A. Lightfoot

Keyword(s):

Genetic Transformation ◽

Agrobacterium Rhizogenes ◽

Corylus Avellana ◽

Root Initiation ◽

Stem Cuttings ◽

Influence Propagation ◽

Rooting Of Cuttings ◽

Shoot Position

Propagation of Corylus avellana stem cuttings may be limited by either root initiation or bud abscission. We divided juvenile shoots of 3 varieties growing in layering beds in mid-July into 4 or 5 3-node cuttings with leaves at the upper two nodes, except that terminal cuttings had one expanded leaf. Cuttings were treated with 5 mM IBA in 50% EtOH, a mixture of A. rhizogenes strains A7 + 22 or left untreated. IBA and bacteria stimulated rooting of cuttings from all shoot positions. Rooting of the terminal cuttings (<50%) was less than that of the sub-terminal cuttings (>80%). Bud retention was <50% on terminal cuttings, nearly 100% on sub-terminal cuttings. Using juvenile stock plants of various varieties, sub-terminal cuttings treated with Agrobacterium or 5 mM IBA may yield 70-90% cuttings with both roots and buds, Agravitropic roots, characteristic of genetic transformation, were observed on Agrobacterium-treated cuttings. Dot blots probed for TL-DNA were negative, however.

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STOCKPLANT ETIOLATION AND SHOOT AGE EFFECTS ON ANATOMY AND ADVENTITIOUS ROOT FORMATION IN STEM CUTTINGS OF CARPINUS BETULUS `FASTIGIATA

HortScience ◽

10.21273/hortsci.25.9.1095d ◽

1990 ◽

Vol 25 (9) ◽

pp. 1095d-1095

Author(s):

Brian K Maynard ◽

Nina L. Bassuk

Keyword(s):

Shoot Growth ◽

Secondary Xylem ◽

Anatomical Study ◽

Adventitious Root Formation ◽

Stem Cuttings ◽

Linear Combinations ◽

Carpinus Betulus ◽

Regression Methods ◽

Root Numbers ◽

Shading Effects

New shoot growth of Carpinus betulus `fastigiata' was treated with stockplant etiolation and stem banding treatments and sampled for anatomical study at intervals over a 16-week period of greening following etiolation. Shading effects on the anatomy of the stem were also investigated. Numerous anatomical changes were noted with stem age and stockplant treatment. Among these were etiolation effects on the lignification of the secondary xylem, thickness of the periderm, and an increase in the percentage of sclereid-free gaps in the perivascular sclerenchyma, Stem banding increased the widths of the cortex and pith. Concomitant propagation studies revealed significant etiolation, shading, and banding effects on rooting percentages and root numbers. Using multiple linear regression methods rooting capacity was modelled best by linear combinations of the widths of the pith, non-lignified secondary xylem, cortical parenchyma and periderm, as well as the percentage of gaps in the sclerenchymatic sheath remaining non-sclerified. It is proposed that the development of sclereids in potential rooting sites reduces rooting potential. The exclusion of light during initial shoot development retards sclereid development by up to 3 months following treatment, which correlates well with observed increases in the rooting potential of etiolated and/or banded stems.

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Callus Formation and Root Initiation in Stem Cuttings of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco)

Canadian Journal of Forest Research ◽

10.1139/x72-023 ◽

1972 ◽

Vol 2 (2) ◽

pp. 121-134 ◽

Cited By ~ 4

Author(s):

J. C. Heaman ◽

J. N. Owens

Keyword(s):

Callus Formation ◽

Douglas Fir ◽

Vascular Cambium ◽

Complex Pattern ◽

Root Initiation ◽

Stem Cuttings ◽

Root Primordia ◽

Weekly Basis ◽

Vascular Tissues ◽

Rooting Response

Changes taking place at the base of stem cuttings of Douglas-fir during callus formation and root initiation are described. Material was taken from nine sources, including trees of different ages and with different histories of rooting response. Gross morphology and anatomy of the cutting bases are described using serial longitudinal sections of samples taken on a weekly basis over a 4-month period. The basal callus proliferated from the lowest cells of the vascular cambium and a complex pattern of differentiating vascular tissues arose within the callus mass. The root primordia arose in this callus in association with the differentiating phloem and wound cambium. Variation in callus formation and root initiation is described both within and between clones.

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Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry

International Journal for Research in Applied Sciences and Biotechnology ◽

10.31033/ijrasb.8.5.21 ◽

2021 ◽

Vol 8 (5) ◽

pp. 147-150

Author(s):

Alam Khan Samim ◽

B. S. Shivakumar ◽

Ganapathi M.

Keyword(s):

Chlorophyll Content ◽

Chlorophyll A ◽

Growth Regulators ◽

Biochemical Parameters ◽

Root Initiation ◽

Stem Cuttings ◽

Chlorophyll B ◽

Total Chlorophyll ◽

Fresh Weight ◽

Total Chlorophyll Content

An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.

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INFLUENCES OF SUBSTRATES ON ROOTING CLEMATIS SOCIALIS STEM CUTTINGS

HortScience ◽

10.21273/hortsci.41.3.497d ◽

2006 ◽

Vol 41 (3) ◽

pp. 497D-497 ◽

Cited By ~ 2

Author(s):

C.N. Johnson ◽

D.J. Eakes ◽

A. N. Wright ◽

J.L Sibley ◽

L.L. Bruner

Keyword(s):

Particle Size ◽

Endangered Species ◽

Root Growth ◽

Genetic Material ◽

Root Initiation ◽

Stem Cuttings ◽

Root Number ◽

Growth Data ◽

Growth And Survival ◽

Root Quality

Clematis socialis Kral, commonly known as the Alabama Leatherflower, is an endangered species with only six known populations in northeast Alabama and northwest Georgia. Cutting propagation of the species will aid in establishing additional self-sustaining populations and provide genetic material for future hybridization and genetic preservation. Such research would also benefit growers, especially native nurseries, who wish to produce this species commercially for its ornamental value. Several experiments were performed to determine the effects of four non-amended substrates on root initiation, root growth, and survival of C. socialis stem cuttings. The four substrates tested included sand, perlite, vermiculite, and a 1 peat (P): 1 pine bark (PB): 1 sand (S) mix (by volume). Some of the best results in the preliminary experiments in 2000 were observed when 2 to 3 node cuttings kept under shade and treated with higher IBA/NAA concentrations were used. In 2004, there was a correlation between root growth and cutting survival and particle size of the substrates. Cuttings rooted in the finer-particle substrates sand and vermiculite had higher cutting survival, root growth, root number, and root quality than those rooted in perlite and the 1 P: 1 PB: 1 S mix. Sand, perlite and vermiculite consistently outperformed the 1 P: 1 PB: 1 S mix which had some of the lowest growth data means. Sand was among the highest performing substrates in all years and it is the most inexpensive and readily available making it the most logical substrate for rooting C. socialis stem cuttings.

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Herbicide Use During Propagation Affects Root Initiation and Development

Journal of Environmental Horticulture ◽

10.24266/0738-2898-11.4.157 ◽

1993 ◽

Vol 11 (4) ◽

pp. 157-159

Author(s):

Charles H. Gilliam ◽

D. Joseph Eakes ◽

John W. Olive

Keyword(s):

Root Initiation ◽

Stem Cuttings ◽

Herbicide Application ◽

Gardenia Jasminoides ◽

Quality Ratings ◽

Herbicide Treatments ◽

Herbicide Use ◽

Root Quality

Abstract Two studies were conducted to evaluate the influence of herbicides on root initiation and development from stem cuttings of three woody landscape species. In the first experiment, Rhododendron obtusum ‘Hino Crimson’ rooting percentage was suppressed with Rout 3G (oxyfluorfen + oryzalin). Both root quality ratings and root lengths were lower for stem cuttings when herbicides were applied compared to untreated controls. Rooting percentages of Rhododendron × ‘Trouper’ and Gardenia jasminoides ‘August Beauty’ were not affected by herbicide application. Root quality ratings and root lengths were generally lower with Rout 3G, OH-2 3G (oxyfluorfen + pendimethlin), Snapshot 2.5TG (trifluralin + isoxaben), and Southern Weed Grass Control 2.68G (pendimethlin) when compared to untreated controls. In the second experiment, depth of sticking Gardenia jasminoides ‘August Beauty’ cuttings and herbicide treatments were evaluated. Cuttings in Rout 3G and Snapshot 2.5 TG treatments had improved root quality ratings when stuck to a depth of 2.5 cm (1.0 in) compared to 1.3 cm (0.5 in); however, all other herbicide treatments had similar root quality ratings to the untreated controls, regardless of depth of sticking.

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Seasonal Variation in Rooting Behaviour of Ginkgo biloba L. Cuttings

Journal of Natural History Museum ◽

10.3126/jnhm.v26i0.14141 ◽

2015 ◽

Vol 26 ◽

pp. 175-180 ◽

Cited By ~ 1

Author(s):

Nabin Bhattarai ◽

Sanu Devi Joshi

Keyword(s):

Ginkgo Biloba ◽

Ex Situ Conservation ◽

Anatomical Study ◽

Soil Condition ◽

Ex Situ ◽

Natural Soil ◽

Stem Cuttings ◽

Secondary Phloem ◽

Cortical Cells ◽

Central Department

Propagation by cuttings is an effective method for ex-situ conservation of plants. In the present study, propagation of Ginkgo biloba L. was conducted using hardwood and semi hard wood cuttings. The cuttings from basal part of the tree were selected for the experiment and planted during rainy (i.e. July) and summer season (i.e. April) at Central Department of Botany, T.U. Cuttings beds were prepared using 3:1 sand and soil. Different concentration of auxins such as IAA, NAA and IBA were found effective in inducing rooting. During July, the stem cuttings treated with 1000 ppm IAA for 30 minutes induced highest percentage (90 %) and statistically signifi cant (P< 0.05) rooting while those treated with same concentration and time induced only 40% rooting during April. Like wise other auxins NAA and IBA induced more rooting during July and less rooting during April. Cuttings planted as control, during July showed 10 % rooting while during April did not show any rooting. Among all the auxins studied, IAA hormone and rainy season was found to be the most effective for rooting of G. biloba cuttings. From the anatomical study it was revealed that vascular cambium, secondary phloem, cortical cells and pericycle cells took part on rooting. Successfully rooted cuttings were then transferred to the natural soil condition. J. Nat. Hist. Mus. Vol. 26, 2012: 175-180

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EFFECT OF SUBSTRATES ON ROOTING OF STEM CUTTINGS OF THE ENDANGERED SPECIES Clematis socialis

HortScience ◽

10.21273/hortsci.40.3.875d ◽

2005 ◽

Vol 40 (3) ◽

pp. 875d-875

Author(s):

C. N. Johnson ◽

D. J. Eakes ◽

L. L. Bruner ◽

A. N. Wright ◽

J. L Sibley

Keyword(s):

Endangered Species ◽

Root Growth ◽

Environmental Conditions ◽

Root Length ◽

Genetic Material ◽

Root Initiation ◽

Stem Cuttings ◽

Root Number ◽

Cutting Propagation ◽

Root Quality

Clematis socialis Kral, also known as the Alabama Leatherflower, is an endangered species with only six known populations in northeast Alabama and northwest Georgia. Cutting propagation of the species would be beneficial for establishing additional self-sustaining populations and providing genetic material for future hybridization. A study conducted in 2000 and 2004 determined the effects of four nonamended substrates on root initiation and growth, as well as survival of C. socialis stem cuttings. Of the four substrates tested, including sand, perlite, vermiculite, and 1:1:1 (by volume) peat (P): pine bark (PB): sand (S), cutting survival was highest in sand in both 2000 and 2004. In 2000, sand also produced the longest roots and highest root quality. Vermiculite produced the longest and most roots and highest root quality in the 2004 study. In 2004, cuttings rooted in fine-particled substrates, such as sand and vermiculite, had higher cutting survival, root growth, root number, and root quality than those rooted in perlite and 1:1:1 (by volume) P:PB:S. The 1:1:1 P:PB:S substrate produced the lowest averages for all data collected in both the 2000 and 2004 studies. Sand was among the two highest performing media in both years, regardless of differences in IBA concentration, misting times, and environmental conditions, making it the overall best substrate for rooting C. socialis stem cuttings. Increasing the concentration of IBA in the rooting solution, providing a cooler environment, and decreasing the number and duration of misting cycles the cuttings received increased cutting survival, root length, root number, and root quality for all four substrates from 2000 to 2004.

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Change in Tissue Mineral Elemental Concentration during Root Initiation and Development of Poinsettia Cuttings

HortScience ◽

10.21273/hortsci.30.3.617 ◽

1995 ◽

Vol 30 (3) ◽

pp. 617-619 ◽

Cited By ~ 8

Author(s):

Sven E. Svenson ◽

Fred T. Davies

Keyword(s):

Adventitious Roots ◽

Elemental Concentration ◽

Euphorbia Pulcherrima ◽

Root Initiation ◽

Stem Cuttings ◽

Root Primordia ◽

Foliar N ◽

Element Concentrations ◽

Propagation Medium

Variation in tissue elemental concentration in apical stem cuttings of `Lilo' and `V-10 Amy' poinsettia (Euphorbia pulcherrima Willd. ex. Klotzch) were studied during the initiation and development of adventitious roots. Changes in selected macro- and micro-element concentrations coincided with root initiation (i.e., Fe, Cu, and Mo accumulated in the basal portions of stem cuttings during early root initiation before root primordia elongation); P, K, Ca, and Mg concentrations declined. During root primordia elongation and root emergence, Fe, Cu, and Mo and Mg, Mn, B, and Zn concentrations continued to increase at the cutting bases, but P and K concentrations remained low compared to when cuttings were initially inserted in the propagation medium. When all cutting of both cultivars had rooted, foliar N, Fe, and Mo concentrations declined, but Cu increased compared to when cuttings were initially propagated.

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