scholarly journals Detection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal

2015 ◽  
Vol 59 (02) ◽  
pp. 185-188 ◽  
Author(s):  
CARLA M.R. VARANDA ◽  
SUSANA J. SANTOS ◽  
MÔNICA D.M. OLIVEIRA ◽  
MARIA IVONE E. CLARA ◽  
MARIA ROSÁRIO F. FÉLIX
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Mottle Virus ◽  
Sweet Potato Virus ◽  
Virus C

scholarly journals Sweet potato feathery mottle virus and Sweet potato virus C from East Timorese and Australian Sweetpotato: Biological and Molecular Properties, and Biosecurity Implications

Plant Disease ◽  
2018 ◽  
Vol 102 (3) ◽  
pp. 589-599 ◽  
Author(s):  
Solomon Maina ◽  
Martin J. Barbetti ◽  
Owain R. Edwards ◽  
Luis de Almeida ◽  
Abel Ximenes ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Sweet Potato ◽  
Potato Virus ◽  
Mottle Virus ◽  
Genetic Connectivity ◽  
Nucleotide Identity ◽  
Fta Cards ◽  
Australian Continent ◽  
Sweet Potato Virus ◽  
Virus C

Sweet potato feathery mottle virus (SPFMV) and Sweet potato virus C (SPVC) isolates from sweetpotato were studied to examine genetic connectivity between viruses from Australia and Southeast Asia. East Timorese samples from sweetpotato were sent to Australia on FTA cards. Shoot and tuberous root samples were collected in Australia and planted in the glasshouse, and scions were graft inoculated to Ipomoea setosa plants. Symptoms in infected sweetpotato and I. setosa plants were recorded. RNA extracts from FTA cards and I. setosa leaf samples were subjected to high-throughput sequencing (HTS). Complete genomic sequences (CS) of SPFMV and SPVC (11 each) were obtained by HTS, and coat protein (CP) genes from them were compared with others from GenBank. SPFMV sequences clustered into two major phylogroups (A and B = RC) and two minor phylogroups (EA[I] and O[II]) within A; East Timorese sequences were in EA(I) and O(II), whereas Australian sequences were in O(II) and B(RC). With SPVC, CP trees provided sufficient diversity to distinguish major phylogroups A and B and six minor phylogroups within A (I to VI); East Timorese sequences were in minor phylogroup I, whereas Australian sequences were in minor phylogroups II and VI and in major phylogroup B. With SPFMV, Aus13B grouped with East Timorese sequence TM64B within minor phylogroup O, giving nucleotide sequence identities of 97.4% (CS) and 98.3% (CP). However, the closest match with an Australian sequence was the 97.6% (CS) and 98.7% (CP) nucleotide identity between Aus13B and an Argentinian sequence. With SPVC, closest nucleotide identity matches between Australian and East Timorese sequences were 94.1% with Aus6a and TM68A (CS) and 96.3% with Aus55-4C and TM64A (CP); however neither pair member belonged to the same minor phylogroup. Also, the closest Australian match was 99.1% (CP) nucleotide identity between Aus4C and New Zealand isolate NZ4-4. These first complete genome sequences of SPFMV and SPVC from sweetpotato plantings in the Australian continent and neighboring Southeast Asia suggest at least two (SPFMV) and three (SPVC) separate introductions to Australia since agriculture commenced more than two centuries ago. These findings have major implications for both healthy stock programs and biosecurity management in relation to pathogen entry into Australia and elsewhere.

scholarly journals New Isolates of Sweet potato feathery mottle virus and Sweet potato virus C: Biological and Molecular Properties, and Recombination Analysis Based on Complete Genomes

Plant Disease ◽  
2018 ◽  
Vol 102 (10) ◽  
pp. 1899-1914 ◽  
Author(s):  
Solomon Maina ◽  
Martin J. Barbetti ◽  
Darren P. Martin ◽  
Owain R. Edwards ◽  
Roger A. C. Jones
Keyword(s):  
Phylogenetic Analysis ◽  
Sweet Potato ◽  
Potato Virus ◽  
High Throughput Sequencing ◽  
Mottle Virus ◽  
Genetic Connectivity ◽  
Recombination Analysis ◽  
Sweet Potato Virus ◽  
Virus C ◽  
A Minor

Sweet potato feathery mottle virus (SPFMV) and Sweet potato virus C (SPVC) isolates were obtained from sweetpotato shoot or tuberous root samples from three widely separated locations in Australia’s tropical north (Cairns, Darwin, and Kununurra). The samples were planted in the glasshouse and scions obtained from the plants were graft inoculated to Ipomoea setosa plants. Virus symptoms were recorded in the field in Kununurra and in glasshouse-grown sweetpotato and I. setosa plants. RNA extracts from I. setosa leaf samples were subjected to high-throughput sequencing. New complete SPFMV (n = 17) and SPVC (n = 6) genomic sequences were obtained and compared with 47 sequences from GenBank. Phylogenetic analysis revealed that the 17 new SPFMV genomes all fitted within either major phylogroup A, minor phylogroup II, formerly O; or major phylogroup B, formerly RC. Major phylogroup A’s minor phylogroup I, formerly EA, only appeared when recombinants were included. Numbers of SPVC genomes were insufficient to subdivide it into phylogroups. Within phylogroup A’s minor phylogroup II, the closest genetic match between an Australian and a Southeast Asian SPFMV sequence was the 97.4% nucleotide identity with an East Timorese sequence. Recombination analysis of the 43 SPFMV and 27 SPVC sequences revealed evidence of 44 recombination events, 16 of which involved interspecies sequence transfers between SPFMV and SPVC and 28 intraspecies transfers, 17 in SPFMV and 11 in SPVC. Within SPFMV, 11 intraspecies recombination events were between different major phylogroups and 6 were between members of the same major phylogroup. Phylogenetic analysis accounting for the detected recombination events within SPFMV sequences yielded evidence of minor phylogroup II and phylogroup B but the five sequences from minor phylogroup I were distributed in two separate groups among the sequences of minor phylogroup II. For the SPVC sequences, phylogenetic analysis accounting for the detected recombination events revealed three major phylogroups (A, B, and C), with major phylogroup A being further subdivided into two minor phylogroups. Within the recombinant genomes of both viruses, their PI, NIa-Pro, NIb, and CP genes contained the highest numbers of recombination breakpoints. The high frequency of interspecies and interphylogroup recombination events reflects the widespread occurrence of mixed SPVC and SPFMV infections within sweetpotato plants. The prevalence of infection in northern Australian sweetpotato samples reinforces the need for improved virus testing in healthy sweetpotato stock programs. Furthermore, evidence of genetic connectivity between Australian and East Timorese SPFMV genomes emphasizes the need for improved biosecurity measures to protect against potentially damaging international virus movements.

scholarly journals Infeksi Alami Pepper Yellow Leaf Curl Virus dan Sweet potato virus C Pada Ubi Jalar di Malang, Jawa Timur

2019 ◽  
Vol 15 (6) ◽  
pp. 248-254
Author(s):  
Tri Asmira Damayanti ◽  
Anastasya Hondo ◽  
Yusmani Prayogo
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Yellow Leaf ◽  
Sweet Potato Virus ◽  
Virus C ◽  
Leaf Curl Virus ◽  
Leaf Curl

Gejala tulang daun kuning (vein yellowing) dan malformasi daun yang diduga disebabkan oleh virus ditemukan pada ubi jalar IR Melati di daerah Kendalpayak, Malang, Jawa Timur. Amplifikasi DNA/cDNA menggunakan primer universal Begomovirus, Potyvirus, dan Cucumovirus menunjukkan positif teramplifikasi DNA dengan primer universal Begomovirus, dan Potyvirus, namun negatif dengan primer universal Cucumovirus. Berdasarkan runutan sikuen nukleotida, gejala tulang daun kuning dan malformasi daun disebabkan oleh infeksi ganda Pepper yellow leaf curl virus (PYLCV) dan Sweet potato virus C (SPVC). Analisis identitas DNA dengan perangkat lunak BioEdit menunjukkan homologi paling tinggi sebesar 98.5% terhadap PYLCV isolat cabai dari Bangli Bali, dan sebesar 98% terhadap SPVC dengan isolat ubi jalar asal Jepang dan Amerika Serikat. Laporan ini merupakan temuan baru infeksi alami PYLCV dan SPVC pada ubi jalar di Indonesia.

scholarly journals Complete Genome Sequences of Sweet potato feathery mottle virus and Sweet potato virus G from Brazil

2018 ◽  
Vol 6 (5) ◽  
Author(s):  
Caroline A. Souza ◽  
Maurício Rossato ◽  
Fernando L. Melo ◽  
Rita C. Pereira-Carvalho
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Complete Genome ◽  
Mottle Virus ◽  
Genome Sequences ◽  
Sweet Potatoes ◽  
Sweet Potato Virus

ABSTRACT In Brazil, Potyvirus species in sweet potatoes have been detected mostly by serology. Here, we report the complete genome sequences of two Potyvirus species, Sweet potato feathery mottle virus strain (SPFMV-UNB-01) and Sweet potato virus G strain (SPVG-UNB-01).

scholarly journals Incidence and distribution of Sweetpotato viruses and their implication on sweetpotato seed system in Malawi

2021 ◽  
Author(s):  
Willard Mbewe ◽  
Andrew Mtonga ◽  
Margret Chiipanthenga ◽  
Kennedy Masamba ◽  
Gloria Chitedze ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Virus Disease ◽  
Disease Incidence ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Foliar Symptoms ◽  
Membrane Enzyme ◽  
Sweet Potato Virus ◽  
Seed System ◽  
Clean Seed

AbstractA survey was carried out in 19 districts to investigate the prevalence and distribution of sweetpotato virus disease (SPVD) and its implication on the sustainability of clean seed system in Malawi. A total of 166 leaf samples were collected and tested for the presence of 8 viruses using nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). SPVD foliar symptoms were observed in 68.42% of the surveyed districts. There were significant variations in disease incidence and severity (p < 0.001) among districts, with the highest incidence in Mulanje (28.34%). Average SPVD severity score was 3.05. NCM-ELISA detected sweet potato feathery mottle virus (SPFMV, 30.54%), sweet potato mild mottle virus (SPMMV, 31.14%), sweet potato mild speckling virus (SPMSV, 16.17%), sweet potato C-6 virus (SPC6V, 13.77%), sweet potato chlorotic stunt virus (SPCSV, 22.16%), sweet potato collusive virus (SPCV, 30.54%), sweet potato virus G (SPVG, 11.38%), cucumber mosaic virus (CMV, 7.78%) either in single or mixed infections. Data from this study indicate a significant SPVD occurrence in the country, and the consequence implications towards national sweetpotato seed system.

Control strategies for sweet potato virus disease in Africa

2004 ◽  
Vol 100 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Richard W. Gibson ◽  
Valentine Aritua ◽  
Emmanuel Byamukama ◽  
Isaac Mpembe ◽  
James Kayongo
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Virus Disease ◽  
Control Strategies ◽  
Potato Virus Disease ◽  
Sweet Potato Virus ◽  
Sweet Potato Virus Disease

scholarly journals The Role of Aphid Abundance, Species Diversity, and Virus Titer in the Spread of Sweetpotato Potyviruses in Louisiana and Mississippi

Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 53-61 ◽  
Author(s):  
E. N. Wosula ◽  
J. A. Davis ◽  
C. A. Clark ◽  
T. P. Smith ◽  
R. A. Arancibia ◽  
...  
Keyword(s):  
Species Diversity ◽  
Virus Infection ◽  
Sweet Potato ◽  
Potato Virus ◽  
Virus Titer ◽  
The United States ◽  
Aphid Species ◽  
Sentinel Plants ◽  
Sweet Potato Virus ◽  
Aphid Abundance

Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG), and Sweet potato virus 2 (SPV2) are sweetpotato (Ipomoea batatas) potyviruses nonpersistently transmitted by aphids. Our objective was to determine how aphid abundance, aphid species diversity, and virus titers relate to the spread of SPFMV, SPVG, and SPV2 in Louisiana and Mississippi sweetpotato fields. The most abundant aphid species were Aphis gossypii, Myzus persicae, Rhopalosiphum padi, and Therioaphis trifolii. Aphids were captured during the entire crop cycle but virus infection of sentinel plants occurred mainly during the months of June to August. SPFMV was more commonly detected than SPVG or SPV2 in sentinel plants. Virus titers for SPFMV were higher in samples beginning in late June. Because significant aphid populations were present during April to June when virus titers were low in sweetpotato and there was very little virus infection of sentinel plants, low virus titers may have limited aphid acquisition and transmission opportunities. This is the first study to comprehensively examine aphid transmission of potyviruses in sweetpotato crops in the United States and includes the first report of R. maidis and R. padi as vectors of SPFMV, though they were less efficient than A. gossypii or M. persicae.

scholarly journals Caracterización de la raza Sweet potato feathery mottle virus que afecta a Ipomoea batatas (L.) Lam. en Argentina y establecimiento de un método rápido de diagnóstico en plantas in vitro

2017 ◽  
Author(s):  
◽  
Paola Analía Faroni
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Mottle Virus ◽  
Ipomoea Nil ◽  
Virus C ◽  
Das Elisa

El cultivo de batata en Argentina ha experimentado una disminución en la superficie plantada y, por ende, en su producción. Actualmente, todas las regiones productoras de batata se encuentran afectadas por una la patología viral denominada “encrespamiento amarillo” (EA), la más grave que se haya presentado hasta la actualidad en el país. El EA es causado por cinco virus, dentro de los cuales se encuentran los potyvirus, Virus C de la batata (SPVC) y Virus del moteado plumoso de la batata (SPFMV) razas RC y O. Estos en infecciones simples no ocasionan problemas en el cultivo, pero en las mixtas llegan a causar mermas en rendimientos superiores al 80%.En el presente trabajo se caracterizó biológica, serológicamente y molecularmente a SPVC que pudo ser aislado de plantas infectadas con SPFMV, otro potyvirus estrechamente relacionado con él. SPVC fue transmitido mediante injerto y mediante inoculación mecánica, en bajo porcentaje en Ipomoea nil e I. setosa. En infecciones simples, se produjo síntomas típicos de infección con SPFMV, en hojas de I. setosa (hospedante alternativo). Complementariamente, se logró purificar el virus y posteriormente se obtuvo el antisuero para el diagnóstico de SPVC, mediante DAS-ELISA, NCM-ELISA e ISEM +D. Los ensayos biológicos y/o serológicos no logran diferenciar SPFMV y SPVC cuando las plantas están infectadas, no resultando fiables para su diagnóstico. Ensayos basados en ácidos nucleícos proporcionan la ventaja de una detección confiable de virus, siendo la sonda de hibridación un instrumento específico para diagnóstico. El presente estudio permitió obtener una sonda de hibridación para detectar específicamente al Sweetpotato virus C, aunque son necesarias pruebas ulteriores que permitan concluir al respecto.

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