scholarly journals To be or not to be? Examples of incorrect identification of autophagic compartments in conventional transmission electron microscopy of mammalian cells

Autophagy ◽  
2008 ◽  
Vol 4 (2) ◽  
pp. 257-260 ◽  
Author(s):  
Eeva-Liisa Eskelinen
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mammalian Cells ◽  
Conventional Transmission Electron Microscopy ◽  
Transmission Electron

High resolution and conventional transmission electron microscopy of Ga2Se3 thin films grown by vapour phase epitaxy

1992 ◽  
Vol 121 (1-2) ◽  
pp. 111-120 ◽  
Author(s):  
A.C. Wright ◽  
J.O. Williams ◽  
A. Krost ◽  
W. Richter ◽  
D.R.T. Zahn
Keyword(s):  
Electron Microscopy ◽  
Thin Films ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Vapour Phase ◽  
Vapour Phase Epitaxy ◽  
Conventional Transmission Electron Microscopy ◽  
Transmission Electron

scholarly journals Applicability of Freeze-Fracture and Cryo-TEM to Complex Liquids

1992 ◽  
Vol 00 (7) ◽  
pp. 9-9
Author(s):  
Janet L. Burns ◽  
Richard J. Spontak
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sample Preparation ◽  
Freeze Fracture ◽  
Traditional Methods ◽  
Conventional Transmission Electron Microscopy ◽  
Transmission Electron ◽  
Multicomponent Complex ◽  
Complex Liquids

Traditional methods of sample preparation and analysis in conventional transmission electron microscopy (TEM) are not readily applicable to multicomponent complex liquids which may contain a wealth of microstructural information. Two techniques which facilitate the study of structure in such liquids are freeze-fracture (FF) TEM and cryo-TEM.

Resolution in conventional transmission electron microscopy

1992 ◽  
Vol 47 (1-3) ◽  
pp. 145-161 ◽  
Author(s):  
Mehmet Sarikaya ◽  
James M. Howe
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Conventional Transmission Electron Microscopy ◽  
Transmission Electron

scholarly journals Three-Dimensional Scanning Transmission Electron Microscopy of Biological Specimens

2010 ◽  
Vol 16 (1) ◽  
pp. 54-63 ◽  
Author(s):  
Niels de Jonge ◽  
Rachid Sougrat ◽  
Brian M. Northan ◽  
Stephen J. Pennycook
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mammalian Cells ◽  
Three Dimensional ◽  
Axial Resolution ◽  
Beam Radiation ◽  
Thin Sections ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission

AbstractA three-dimensional (3D) reconstruction of the cytoskeleton and a clathrin-coated pit in mammalian cells has been achieved from a focal-series of images recorded in an aberration-corrected scanning transmission electron microscope (STEM). The specimen was a metallic replica of the biological structure comprising Pt nanoparticles 2–3 nm in diameter, with a high stability under electron beam radiation. The 3D dataset was processed by an automated deconvolution procedure. The lateral resolution was 1.1 nm, set by pixel size. Particles differing by only 10 nm in vertical position were identified as separate objects with greater than 20% dip in contrast between them. We refer to this value as the axial resolution of the deconvolution or reconstruction, the ability to recognize two objects, which were unresolved in the original dataset. The resolution of the reconstruction is comparable to that achieved by tilt-series transmission electron microscopy. However, the focal-series method does not require mechanical tilting and is therefore much faster. 3D STEM images were also recorded of the Golgi ribbon in conventional thin sections containing 3T3 cells with a comparable axial resolution in the deconvolved dataset.

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