scholarly journals MEK1/2 Regulates Microtubule Organization, Spindle Pole Tethering and Asymmetric Division During Mouse Oocyte Meiotic Maturation

Cell Cycle ◽  
2007 ◽  
Vol 6 (3) ◽  
pp. 330-338 ◽  
Author(s):  
Ling-Zhu Yu ◽  
Bo Xiong ◽  
Wen-Xue Gao ◽  
Chun-Min Wang ◽  
Zhi-Sheng Zhong ◽  
...  
Keyword(s):  
Asymmetric Division ◽  
Spindle Pole ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Microtubule Organization ◽  
Oocyte Meiotic Maturation

mInscuteable regulates meiotic spindle organization during mouse oocyte meiotic maturation

Zygote ◽  
2019 ◽  
Vol 28 (1) ◽  
pp. 45-50
Author(s):  
Zhuoni Xiao ◽  
Jiali Peng ◽  
Meiting Xie ◽  
Jing Yang ◽  
Wangming Xu
Keyword(s):  
Oocyte Maturation ◽  
Germinal Vesicle ◽  
Asymmetric Division ◽  
Mouse Oocyte ◽  
Meiotic Spindle ◽  
Meiotic Maturation ◽  
Cellular Polarity ◽  
Germinal Vesicle Breakdown ◽  
Key Events ◽  
Oocyte Meiotic Maturation

SummaryEstablishment of cellular polarity is one of the key events during oocyte maturation. Inscuteable (Insc) has been identified as a key regulator of cell polarity during asymmetric division in Drosophila. However, the function of its evolutionarily conserved mammalian homologue, mInscuteable (mInsc), in mouse meiotic maturation is not clear. In this study, we investigated the roles of mInsc in mouse oocyte maturation. mInsc was detected at all stages of oocyte maturation. The protein level of mInsc was slightly higher at the germinal vesicle breakdown (GVBD) stage and remained constant during mouse oocyte maturation. The subcellular localization of mInsc overlapped with spindle microtubules. Disruption of microtubules and microfilaments caused changes in the localization of mInsc. Depletion or overexpression of mInsc significantly decreased the maturation rates of mouse oocytes. Depletion of mInsc significantly affected asymmetric division, spindle assembly, alignments of chromosomes and actin cap formation. Taken together, our results demonstrated that mInsc regulates meiotic spindle organization during mouse meiotic maturation.

scholarly journals Maternal mRNAs with distinct 3′ UTRs define the temporal pattern ofCcnb1synthesis during mouse oocyte meiotic maturation

2017 ◽  
Vol 31 (13) ◽  
pp. 1302-1307 ◽  
Author(s):  
Ye Yang ◽  
Cai-Rong Yang ◽  
Seung Jin Han ◽  
Enrico Maria Daldello ◽  
Ara Cho ◽  
...  
Keyword(s):  
Temporal Pattern ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maternal Mrnas ◽  
Oocyte Meiotic Maturation

Centrosome and microtubule dynamics during meiotic progression in the mouse oocyte

1991 ◽  
Vol 100 (2) ◽  
pp. 289-298 ◽  
Author(s):  
S.M. Messinger ◽  
D.F. Albertini
Keyword(s):  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Microtubule Nucleation ◽  
Microtubule Organization ◽  
Double Labeling ◽  
Meiotic Progression ◽  
Tubulin Antibodies ◽  
Discrete Populations ◽  
Meiosis I

The disposition, function and fate of centrosomes were analysed in mouse oocytes undergoing in vitro meiotic maturation, using multiple-label fluorescence microscopy. Oocytes fixed at various points during meiotic progression were double labeled with either human centrosome-specific antibody, 5051, and anti-tubulin antibodies or 5051 and MPM-2 antibodies in order to evaluate the microtubule nucleation capacity and phosphorylation status of centrosomes during this process. Double labeling with anti-tubulin antibodies revealed two populations of centrosomes that undergo stage-specific changes in number, location and microtubule nucleation capacity in relation to spindle assembly and cytoplasmic events. Specifically, one population was consistently associated with chromatin throughout meiotic maturation whereas a second population of cytoplasmic centrosomes exhibited maximal numbers and nucleation capacity at prometaphase and anaphase of meiosis-I. Quantitative evaluation of cytoplasmic centrosomes indicated increased numbers during the transition from diakinesis to prometaphase and metaphase to anaphase and total disappearance during telophase. Colocalization studies with MPM-2 revealed that centrosomes were always phosphorylated. However, at metaphase of meiosis I and II the microtubule nucleation capacity of centrosomes was diminished. These results suggest the existence of two discrete populations of centrosomes in the mouse oocyte that are coordinately regulated to subserve aspects of microtubule organization relative to both nuclear and cytoplasmic events.

Diethylstilbestrol exposure disrupts mouse oocyte meiotic maturation in vitro through affecting spindle assembly and chromosome alignment

Chemosphere ◽  
2020 ◽  
Vol 249 ◽  
pp. 126182 ◽  
Author(s):  
Zhi-Ming Ding ◽  
Li-Ping Hua ◽  
Muhammad Jamil Ahmad ◽  
Muhammad Safdar ◽  
Fan Chen ◽  
...  
Keyword(s):  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maturation In Vitro ◽  
Chromosome Alignment ◽  
Oocyte Meiotic Maturation

JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation

2011 ◽  
Vol 17 (2) ◽  
pp. 197-205 ◽  
Author(s):  
Xin Huang ◽  
Jing-Shan Tong ◽  
Zhen-Bo Wang ◽  
Cai-Rong Yang ◽  
Shu-Tao Qi ◽  
...  
Keyword(s):  
Polar Body ◽  
Specific Inhibitor ◽  
Germinal Vesicle ◽  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Cytoplasmic Microtubule ◽  
Spindle Poles ◽  
First Polar Body ◽  
Oocyte Meiotic Maturation

AbstractIt is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. In this study, we found that no specific JNK2 signal was detected in germinal vesicle stage. JNK2 was associated with the spindles especially the spindle poles and cytoplasmic microtubule organizing centers at prometaphase I, metaphase I, and metaphase II stages. JNK2 became diffusely distributed and associated with the midbody at telophase I stage. Injection of myc-tagged JNK2α1 mRNA into oocytes also revealed its localization on spindle poles. The association of JNK2 with spindle poles was further confirmed by colocalization with the centrosomal proteins, γ-tubulin and Plk1. Nocodazole treatment showed that JNK2 may interact with Plk1 to regulate the spindle assembly. Then we investigated the possible function of JNK2 by JNK2 antibody microinjection and JNK specific inhibitor SP600125 treatment. These two manipulations caused abnormal spindle formation and decreased the rate of first polar body (PB1) extrusion. In addition, inhibition of JNK2 resulted in impaired localization of Plk1. Taken together, our results suggest that JNK2 plays an important role in spindle assembly and PB1 extrusion during mouse oocyte meiotic maturation.

Global Analysis of Maternal mRNA Translation during Mouse Oocyte Meiotic Maturation.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 327-327
Author(s):  
Jing Chen ◽  
Collin Melton ◽  
NaYoung Suh ◽  
Robert Blelloch ◽  
Marco Conti
Keyword(s):  
Global Analysis ◽  
Mrna Translation ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Maternal Mrna ◽  
Oocyte Meiotic Maturation

Actin and Myosin-II Regulate Cortical Mechanical Properties During Mouse Oocyte Meiotic Maturation.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 290-290
Author(s):  
Hyo J. Lee ◽  
Douglas N. Robinson ◽  
Janice P. Evans
Keyword(s):  
Mechanical Properties ◽  
Myosin Ii ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Oocyte Meiotic Maturation

scholarly journals Mitofusin-2 is required for mouse oocyte meiotic maturation

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Jing-Hua Zhang ◽  
Teng Zhang ◽  
Si-Hua Gao ◽  
Ke Wang ◽  
Xiu-Yan Yang ◽  
...  
Keyword(s):  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Mitofusin 2 ◽  
Oocyte Meiotic Maturation

Methoxychlor exposure induces oxidative stress and affects mouse oocyte meiotic maturation

2016 ◽  
Vol 83 (9) ◽  
pp. 768-779 ◽  
Author(s):  
Yu Liu ◽  
Ya-Long Wang ◽  
Ming-Huang Chen ◽  
Zhen Zhang ◽  
Bai-Hui Xu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mouse Oocyte ◽  
Meiotic Maturation ◽  
Oocyte Meiotic Maturation
Sign in / Sign up

Export Citation Format

Share Document