scholarly journals P-class pentatricopeptide repeat proteins are required for efficient 5′ end formation of plant mitochondrial transcripts

RNA Biology ◽  
2013 ◽  
Vol 10 (9) ◽  
pp. 1511-1519 ◽  
Author(s):  
Stefan Binder ◽  
Katrin Stoll ◽  
Birgit Stoll
Keyword(s):  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
Mitochondrial Transcripts ◽  
Pentatricopeptide Repeat Proteins

scholarly journals Three new pentatricopeptide repeat proteins facilitate the splicing of mitochondrial transcripts and complex I biogenesis in Arabidopsis

2018 ◽  
Vol 69 (21) ◽  
pp. 5131-5140 ◽  
Author(s):  
Chuande Wang ◽  
Fabien Aubé ◽  
Martine Quadrado ◽  
Céline Dargel-Graffin ◽  
Hakim Mireau
Keyword(s):  
Complex I ◽  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
Mitochondrial Transcripts ◽  
Pentatricopeptide Repeat Proteins

scholarly journals In Arabidopsis thaliana mitochondria 5′ end polymorphisms of nad4L-atp4 and nad3-rps12 transcripts are linked to RNA PROCESSING FACTORs 1 and 8

2021 ◽  
Author(s):  
Sarah Schleicher ◽  
Stefan Binder
Keyword(s):  
Arabidopsis Thaliana ◽  
Rna Processing ◽  
Degradation Products ◽  
Underlying Mechanism ◽  
Transcriptional Level ◽  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
P Type ◽  
Pentatricopeptide Repeat Proteins ◽  
Processing Factors

Abstract Key message RNA PROCESSING FACTORs 1 AND 8 (RPF1 and RPF8), both restorer of fertility like pentatricopeptide repeat proteins, are required for processing of dicistronic nad4L-atp4 and nad3-rps12 transcripts in Arabidopsis mitochondria. Abstract In mitochondria of Arabidopsis thaliana (Arabidopsis), the 5′ termini of many RNAs are generated on the post-transcriptional level. This process is still poorly understood in terms of both the underlying mechanism as well as proteins required. Our studies now link the generation of polymorphic 5′ extremities of the dicistronic nad3-rps12 and nad4L-atp4 transcripts to the function of the P-type pentatricopeptide repeat proteins RNA PROCESSING FACTORs 8 (RPF8) and 1 (RPF1). RPF8 is required to generate the nad3-rps12 -141 5′ end in ecotype Van-0 whereas the RPF8 allele in Col has no function in the generation of any 5′ terminus of this transcript. This observation strongly suggests the involvement of an additional factor in the generation of the -229 5′ end of nad3-rps12 transcripts in Col. RPF1, previously found to be necessary for the generation of the -228 5′ end of the major 1538 nucleotide-long nad4 mRNAs, is also important for the formation of nad4L-atp4 transcripts with a 5′ end at position -318 in Col. Many Arabidopsis ecotypes contain inactive RPF1 alleles resulting in the accumulation of various low abundant nad4L-atp4 RNAs which might represent precursor and/or degradation products. Some of these ecotypes accumulate major, but slightly smaller RNA species. The introduction of RPF1 into these lines not only establishes the formation of the major nad4L-atp4 dicistronic mRNA with the -318 5′ terminus, the presence of this gene also suppresses the accumulation of most alternative nad4L-atp4 RNAs. Beside RPF1, several other factors contribute to nad4L-atp4 transcript formation.

scholarly journals The E domains of pentatricopeptide repeat proteins from different organelles are not functionally equivalent for RNA editing

2013 ◽  
Vol 74 (6) ◽  
pp. 935-945 ◽  
Author(s):  
Anne-Laure Chateigner-Boutin ◽  
Catherine Colas des Francs-Small ◽  
Sota Fujii ◽  
Kenji Okuda ◽  
Sandra K. Tanz ◽  
...  
Keyword(s):  
Rna Editing ◽  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
Pentatricopeptide Repeat Proteins

scholarly journals A Combinatorial Amino Acid Code for RNA Recognition by Pentatricopeptide Repeat Proteins

PLoS Genetics ◽  
2012 ◽  
Vol 8 (8) ◽  
pp. e1002910 ◽  
Author(s):  
Alice Barkan ◽  
Margarita Rojas ◽  
Sota Fujii ◽  
Aaron Yap ◽  
Yee Seng Chong ◽  
...  
Keyword(s):  
Amino Acid ◽  
Rna Recognition ◽  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
Amino Acid Code ◽  
Pentatricopeptide Repeat Proteins

scholarly journals Identification of Pentatricopeptide Repeat Proteins in the Model OrganismDictyostelium discoideum

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Sam Manna ◽  
Jessica Brewster ◽  
Christian Barth
Keyword(s):  
Dictyostelium Discoideum ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Gene Products ◽  
Antisense Inhibition ◽  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
Ppr Proteins ◽  
Pentatricopeptide Repeat Proteins ◽  
Encoding Genes

Pentatricopeptide repeat (PPR) proteins are RNA binding proteins with functions in organelle RNA metabolism. They are found in all eukaryotes but have been most extensively studied in plants. We report on the identification of 12 PPR-encoding genes in the genome of the protistDictyostelium discoideum, with potential homologs in other members of the same lineage and some predicted novel functions for the encoded gene products in protists. For one of the gene products, we show that it localizes to the mitochondria, and we also demonstrate that antisense inhibition of its expression leads to slower growth, a phenotype associated with mitochondrial dysfunction.

scholarly journals Pentatricopeptide Repeat Proteins in Trypanosoma brucei Function in Mitochondrial Ribosomes

2007 ◽  
Vol 27 (19) ◽  
pp. 6876-6888 ◽  
Author(s):  
Mascha Pusnik ◽  
Ian Small ◽  
Laurie K. Read ◽  
Thomas Fabbro ◽  
André Schneider
Keyword(s):  
Trypanosoma Brucei ◽  
Large Subunit ◽  
Direct Interaction ◽  
Pentatricopeptide Repeat ◽  
Amino Acid Motif ◽  
Mitochondrial Ribosomes ◽  
Repeat Proteins ◽  
Ppr Proteins ◽  
Pentatricopeptide Repeat Proteins ◽  
Large Subunit Rrna

ABSTRACT The pentatricopeptide repeat (PPR), a degenerate 35-amino-acid motif, defines a novel eukaryotic protein family. Plants have 400 to 500 distinct PPR proteins, whereas other eukaryotes generally have fewer than 5. The few PPR proteins that have been studied have roles in organellar gene expression, probably via direct interaction with RNA. Here we show that the parasitic protozoan Trypanosoma brucei encodes 28 distinct PPR proteins, an extraordinarily high number for a nonplant organism. A comparative analysis shows that seven out of eight selected PPR proteins are mitochondrially localized and essential for oxidative phosphorylation. Six of these are required for the stabilization of mitochondrial rRNAs and, like ribosomes, are associated with the mitochondrial membranes. Furthermore, one of the PPR proteins copurifies with the large subunit rRNA. Finally, ablation of all of the PPR proteins that were tested induces degradation of the other PPR proteins, indicating that they function in concert. Our results show that a significant number of trypanosomal PPR proteins are individually essential for the maintenance and/or biogenesis of mitochondrial rRNAs.

scholarly journals Pentatricopeptide Repeat Proteins Stimulate mRNA Adenylation/Uridylation to Activate Mitochondrial Translation in Trypanosomes

2011 ◽  
Vol 42 (1) ◽  
pp. 106-117 ◽  
Author(s):  
Inna Aphasizheva ◽  
Dmitri Maslov ◽  
Xiaorong Wang ◽  
Lan Huang ◽  
Ruslan Aphasizhev
Keyword(s):  
Pentatricopeptide Repeat ◽  
Mitochondrial Translation ◽  
Repeat Proteins ◽  
Pentatricopeptide Repeat Proteins

scholarly journals The Pentatricopeptide Repeat Proteins TANG2 and ORGANELLE TRANSCRIPT PROCESSING439 Are Involved in the Splicing of the Multipartite nad5 Transcript Encoding a Subunit of Mitochondrial Complex I

2014 ◽  
Vol 165 (4) ◽  
pp. 1409-1416 ◽  
Author(s):  
Catherine Colas des Francs-Small ◽  
Andéol Falcon de Longevialle ◽  
Yunhai Li ◽  
Elizabeth Lowe ◽  
Sandra K. Tanz ◽  
...  
Keyword(s):  
Complex I ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
A Subunit ◽  
Pentatricopeptide Repeat Proteins

Identification of pentatricopeptide repeat proteins in Trypanosoma brucei

2006 ◽  
Vol 150 (1) ◽  
pp. 37-45 ◽  
Author(s):  
Melissa K. Mingler ◽  
Andrea M. Hingst ◽  
Sandra L. Clement ◽  
Laura E. Yu ◽  
Larissa Reifur ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Pentatricopeptide Repeat ◽  
Repeat Proteins ◽  
Pentatricopeptide Repeat Proteins
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