scholarly journals Aralia cordata Extract Activates NF-κB and MAPK Signaling Pathways and Induces Pro-inflammatory Changes in RAW264.7 Macrophages

2019 ◽  
Vol 49 (4) ◽  
pp. 153
Author(s):  
Dong-Won Seo ◽  
Yong-Il Cho ◽  
Young-Joo Yi ◽  
Sang-Myeong Lee
Keyword(s):  
Signaling Pathways ◽  
Mapk Signaling ◽  
Aralia Cordata ◽  
Raw264.7 Macrophages ◽  
Inflammatory Changes ◽  
Mapk Signaling Pathways

scholarly journals Immune activation of murine RAW264.7 macrophages by sonicated and alkalized paramylon from Euglena gracilis

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Qingqing Guo ◽  
Decheng Bi ◽  
Mingcan Wu ◽  
Boming Yu ◽  
Lang Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune System ◽  
Signaling Pathways ◽  
Euglena Gracilis ◽  
Mapk Signaling ◽  
Regulation Mechanism ◽  
Dependent Manner ◽  
Raw264.7 Macrophages ◽  
Therapeutic Benefits ◽  
Mapk Signaling Pathways

Abstract Background Euglena is a new super health food resource that is rich in the natural polysaccharide paramylon, a linear β-1,3-glucan with various biological activities including activity on the immune system in different cell lines and animals. Despite these reports, the immune regulation mechanism of paramylon is still unclear. Results We investigate the signaling pathways paramylon impacts in immune macrophages. In RAW264.7 macrophages, sonicated and alkalized paramylon oligomers up-regulated inducible nitric oxide synthase (iNOS) and increased secretion of nitric oxide (NO), interleukin (IL)-6 and tumor necrosis factor (TNF)-α, in a concentration-dependent manner. In addition, paramylon activated the nuclear factor-κB(NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways and inhibiting these pathways attenuated the paramylon-induced secretion of the above immune-mediators. Conclusions These results demonstrate that Euglena gracilis paramylon modulates the immune system via activation of the NF-κB and MAPK signaling pathways and thus has potential therapeutic benefits.

scholarly journals Immune activation of murine RAW264.7 macrophages by sonicated and alkalized paramylon from Euglena gracilis

2020 ◽  
Author(s):  
Qingqing Guo ◽  
Decheng Bi ◽  
Mingcan Wu ◽  
Boming Yu ◽  
Lang Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune System ◽  
Signaling Pathways ◽  
Euglena Gracilis ◽  
Mapk Signaling ◽  
Regulation Mechanism ◽  
Dependent Manner ◽  
Raw264.7 Macrophages ◽  
Therapeutic Benefits ◽  
Mapk Signaling Pathways

Abstract Background: Euglena is a new super health food resource that is rich in the natural polysaccharide paramylon, a linear β-1,3-glucan with various biological activities including activity on the immune system in different cell lines and animals. Despite these reports, the immune regulation mechanism of paramylon is still unclear. Results: We investigate the signaling pathways paramylon impacts in immune macrophages. In RAW264.7 macrophages, sonicated and alkalized paramylon oligomers up-regulated inducible nitric oxide synthase (iNOS) and increased secretion of nitric oxide (NO), interleukin (IL)-6 and tumor necrosis factor (TNF)-α, in a concentration-dependent manner. In addition, paramylon activated the nuclear factor-κB(NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways and inhibiting these pathways attenuated the paramylon-induced secretion of the above immune-mediators. Conclusions: These results demonstrate that Euglena gracilis paramylon modulates the immune system via activation of the NF-κB and MAPK signaling pathways and thus has potential therapeutic benefits.

scholarly journals Immune activation of murine RAW264.7 macrophages by sonicated and alkalized paramylon from Euglena gracilis

2019 ◽  
Author(s):  
Qingqing Guo ◽  
Decheng Bi ◽  
Mingcan Wu ◽  
Boming Yu ◽  
Lang Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune System ◽  
Signaling Pathways ◽  
Euglena Gracilis ◽  
Mapk Signaling ◽  
Regulation Mechanism ◽  
Dependent Manner ◽  
Raw264.7 Macrophages ◽  
Therapeutic Benefits ◽  
Mapk Signaling Pathways

Abstract Background: Euglena as a new super health food resource that is rich in the natural polysaccharide paramylon, a linear β-1,3-glucan with various biological activities including activity on the immune system in different cell lines and animals. Despite these reports, the immune regulation mechanism of paramylon is still unclear. Results: We investigate the signaling pathways paramylon impacts in immune macrophages. In RAW264.f:7 macrophages, sonicated and alkalized paramylon oligomers up-regulated inducible nitric oxide synthase (iNOS) and increased secretion of nitric oxide (NO), interleukin (IL)-6 and tumor necrosis factor (TNF)-α, in a concentration-dependent manner. In addition, paramylon activated the nuclear factor-κB(NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways and inhibiting these pathways attenuated the paramylon-induced secretion of the above immune-mediators. Conclusions: These results demonstrate that Euglena gracilis paramylon modulates the immune system via activation of the NF-κB and MAPK signaling pathways and thus has potential therapeutic benefits.

scholarly journals Frutescone O from Baeckea frutescens Blocked TLR4-Mediated Myd88/NF-κB and MAPK Signaling Pathways in LPS Induced RAW264.7 Macrophages

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaobing Lin ◽  
Junhan Zhang ◽  
Decai Fan ◽  
Jiqin Hou ◽  
Hao Wang ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Molecular Mechanisms ◽  
Mapk Signaling ◽  
Raw264.7 Cells ◽  
Raw264.7 Macrophages ◽  
Anti Inflammatory ◽  
Baeckea Frutescens ◽  
Myd88 Signaling ◽  
Myeloid Differentiation Factor ◽  
Mapk Signaling Pathways

Frutescone O was isolated from the aerial parts of Baeckea frutescens L., which was commonly used as a folk medicinal material for treating anti-inflammatory disease in South East Asia. This study aimed to investigate the anti-inflammatory activity and related signaling cascade of Frutescone O (Fru) in LPS induced RAW264.7 cells. The anti-inflammation activity of Frutescone O was determined according to the inhibitory effects on the secretion of nitric oxide (NO), expression of inducible NO synthase, and pro-inflammatory cytokines. The regulation of Myeloid differentiation factor 88 (Myd88), inhibition of NF-κB, and MAPK pathways were further investigated for molecular mechanisms. Fru significantly decreased the expression of iNOS and the production of NO in LPS-stimulated RAW264.7 cells. It also dose-dependently suppressed LPS induced expression of IL-1β, IL-6, and TNF-α. Furthermore, Fru remarkably inhibited the upregulation of NF-κB (p50) expression in the nucleus and the phosphorylation ratio of p38, JNK, ERK, and Myd88 signaling protein. The molecular docking and cellular thermal shift assay (CETSA) results indicated that Fru participated in a robust and stable interaction with the active site of TLR4-MD2. Thus, Fru suppressed the LPS induced inflammation in RAW264.7 cells by blocking the TLR4 mediated signal transduction through the NF-κB and MAPK signaling pathways and inhibiting the Myd88 and iNOS expression.

scholarly journals Immune activation of murine RAW264.7 macrophages by sonicated and alkalized paramylon from Euglena gracilis

2020 ◽  
Author(s):  
Qingqing Guo ◽  
Decheng Bi ◽  
Mingcan Wu ◽  
Boming Yu ◽  
Lang Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune System ◽  
Signaling Pathways ◽  
Euglena Gracilis ◽  
Mapk Signaling ◽  
Regulation Mechanism ◽  
Dependent Manner ◽  
Raw264.7 Macrophages ◽  
Therapeutic Benefits ◽  
Mapk Signaling Pathways

Abstract Background: Euglena is a new super health food resource that is rich in the natural polysaccharide paramylon, a linear β-1,3-glucan with various biological activities including activity on the immune system in different cell lines and animals. Despite these reports, the immune regulation mechanism of paramylon is still unclear. Results: We investigate the signaling pathways paramylon impacts in immune macrophages. In RAW264.7 macrophages, sonicated and alkalized paramylon oligomers up-regulated inducible nitric oxide synthase (iNOS) and increased secretion of nitric oxide (NO) , interleukin (IL)-6 and tumor necrosis factor (TNF)- α, in a concentration-dependent manner. In addition, paramylon activated the nuclear factor-κB(NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways and inhibiting these pathways attenuated the paramylon-induced secretion of the above immune-mediators. Conclusions: These results demonstrate that Euglena gracilis paramylon modulates the immune system via activation of the NF-κB and MAPK signaling pathways and thus has potential therapeutic benefits.

scholarly journals Immune activation of murine RAW264.7 macrophages by sonicated and alkalized paramylon from Euglena gracilis

2020 ◽  
Author(s):  
Qingqing Guo ◽  
Decheng Bi ◽  
Mingcan Wu ◽  
Boming Yu ◽  
Lang Hu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune System ◽  
Signaling Pathways ◽  
Euglena Gracilis ◽  
Mapk Signaling ◽  
Regulation Mechanism ◽  
Dependent Manner ◽  
Raw264.7 Macrophages ◽  
Therapeutic Benefits ◽  
Mapk Signaling Pathways

Abstract Background: Euglena is a new super health food resource that is rich in the natural polysaccharide paramylon, a linear β-1,3-glucan with various biological activities including activity on the immune system in different cell lines and animals. Despite these reports, the immune regulation mechanism of paramylon is still unclear. Results: We investigate the signaling pathways paramylon impacts in immune macrophages. In RAW264.7 macrophages, sonicated and alkalized paramylon oligomers up-regulated inducible nitric oxide synthase (iNOS) and increased secretion of nitric oxide (NO) , interleukin (IL)-6 and tumor necrosis factor (TNF)- α, in a concentration-dependent manner. In addition, paramylon activated the nuclear factor-κB(NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways and inhibiting these pathways attenuated the paramylon-induced secretion of the above immune-mediators. Conclusions: These results demonstrate that Euglena gracilis paramylon modulates the immune system via activation of the NF-κB and MAPK signaling pathways and thus has potential therapeutic benefits.

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