Optimization of submerged culture conditions for mycelial polysaccharide production in Cordyceps pruinosa

2004 ◽  
Vol 39 (12) ◽  
pp. 2241-2247 ◽  
Author(s):  
Jian Hui Xiao ◽  
Dai Xiong Chen ◽  
Yu Xiao ◽  
Jin Wei Liu ◽  
Zu Lin Liu ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Culture Conditions ◽  
Polysaccharide Production

Investigation of culture conditions by Response Surface Methodology and kinetic modeling for exopolysaccharide production by Klebsiella oxytoca ICCF 419 strain, using lactose as substrate

2020 ◽  
Vol 25 (6) ◽  
pp. 2033-2044
Author(s):  
CATERINA TOMULESCU ◽  
◽  
MIŞU MOSCOVICI ◽  
ROXANA MĂDĂLINA STOICA ◽  
GABRIEL BUJOR ALBU ◽  
...  
Keyword(s):  
Citric Acid ◽  
Klebsiella Oxytoca ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Biomass Growth ◽  
Initial Value ◽  
Substrate Consumption ◽  
Polysaccharide Production ◽  
Crude Polysaccharide ◽  
Central Composite

The objective of this work was to optimize the bioprocess parameters, using Klebsiella oxytoca ICCF 419 to obtain an exopolysaccharide based on lactose as substrate. A kinetic study was employed and Logistic and Gompertz models were applied to describe the polysaccharide production, in relation with biomass growth and substrate consumption. The RSM methodology based on Central Composite Rotatable Design was used to evaluate and optimize the effect of lactose, corn extract, KH2PO4 and citric acid concentrations as independent variables on the polysaccharide production, biomass growth and substrate consumption as the response functions. The interaction effects and optimal parameters were obtained using Design Expert Software (version 9.0.6.2). The significance of the variables and their interactions was tested by means of ANOVA analysis with a 99% confidence level. The optimum culture conditions were determined and the model prediction was compared with experimental results. At an initial value of 23.45 for the C/N in the fermentation medium, the strain produces 17.41 g/L of crude polysaccharide and 2.53 g/L dry biomass. The EPS production was significantly influenced by lactose, corn extract and KH2PO4, while the citric acid had no influence. The biomass growth was influenced by the corn extract, KH2PO4 and citric acid.

Optimization of submerged culture conditions for exopolysaccharide production in Sarcodon aspratus (Berk) S.lto TG-3

2004 ◽  
Vol 20 (7) ◽  
pp. 767-773 ◽  
Author(s):  
Ji Hoon Joo ◽  
Jong Min Lim ◽  
Hyun Oh Kim ◽  
Sang Woo Kim ◽  
Hye Jin Hwang ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Culture Conditions ◽  
Exopolysaccharide Production

Optimization of Culture Conditions on Mycelial Grown in Submerged Culture of Cordyceps militaris

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jian Dong Cui ◽  
Li Qiang Yuan
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Fold Increase ◽  
Culture Conditions ◽  
Statistical Experimental Design ◽  
Fermentation Time ◽  
Original Culture ◽  
Production Values ◽  
Validation Experiment ◽  
Optimal Region

Statistical experimental design strategy (SES) was employed to optimize the culture conditions for the mycelial growth of C. militaris by submerged culture. Using Plackett-Burman design (PBD), liquid volumes in flask and fermentation time were identified as the significant variables that would highly influence mycelial growth, and these variables were subsequently optimized using response surface method (RSM). The steepest ascent method was used to access the optimal region of the culture condition. The results showed that the optimum values of the tested variables were 47.3 mL of liquid volumes in flask and 7.6 days of fermentation time. Under optimized culture conditions, the mycelial production was enhanced from 10.33 to 19.97 g/L. In comparison with that of original culture conditions, 1.9-fold increase was obtained. The validation experiment showed that the experimentally determined production values were in close agreement with the statistically predicted ones.

scholarly journals Enhancement of 2,3-Butanediol Production byKlebsiella oxytocaPTCC 1402

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Maesomeh Anvari ◽  
Mohammad Reza Safari Motlagh
Keyword(s):  
Acetic Acid ◽  
Taguchi Method ◽  
Submerged Culture ◽  
Klebsiella Oxytoca ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Medium Composition ◽  
Operating Parameters ◽  
Optimal Operating ◽  
Mixing Intensity

Optimal operating parameters of 2,3-Butanediol production usingKlebsiella oxytocaunder submerged culture conditions are determined by using Taguchi method. The effect of different factors including medium composition, pH, temperature, mixing intensity, and inoculum size on 2,3-butanediol production was analyzed using the Taguchi method in three levels. Based on these analyses the optimum concentrations of glucose, acetic acid, and succinic acid were found to be 6, 0.5, and 1.0 (% w/v), respectively. Furthermore, optimum values for temperature, inoculum size, pH, and the shaking speed were determined as 37°C, 8 (g/L), 6.1, and 150 rpm, respectively. The optimal combinations of factors obtained from the proposed DOE methodology was further validated by conducting fermentation experiments and the obtained results revealed an enhanced 2,3-Butanediol yield of 44%.

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