Anodized Nanotubular TiO2 Structures Significantly Improve Titanium Implant Materials In Vitro and In Vivo

2012 ◽  
Vol 03 (04) ◽  
Author(s):  
Kim M. Kummer
Keyword(s):  
Titanium Implant ◽  
Implant Materials

The Use of the Chorioallantoic Membrane (CAM) of the Embryonic Chick for the Direct Assessment of Implant Toxicity

1985 ◽  
Vol 13 (4) ◽  
pp. 261-266
Author(s):  
P.P. Monro ◽  
D.P. Knight ◽  
W.S. Pringle ◽  
D.M. Fyfe ◽  
J.R. Shearer
Keyword(s):  
Chorioallantoic Membrane ◽  
In Vitro Techniques ◽  
Implant Materials ◽  
Metal Foils ◽  
Complex Interactions ◽  
Cobalt Nickel ◽  
Histological Criteria ◽  
Fluid Environment

The toxicity of implant materials requires investigation prior to clinical use. We have developed a method where materials are directly applied to the chorioallantoic membrane (CAM) of 9-day-old chick embryos and toxicity is assessed using histological criteria. We evaluated the method using metal foils. The number and organisation of fibroblasts seemed to be the most useful criteria for assessing metal toxicity. Differences were greatest after 10 days of culture on the CAM. The method is sensitive enough to enable us to discriminate between the less toxic aluminium and titanium and the highly toxic cobalt, nickel and tungsten. The proposed method has advantages over in vitro techniques which provide an abnormal fluid environment and in which the more complex interactions that are possible between implant materials and tissue in vivo cannot be modelled.

Formation and In Vitro and In Vivo Evaluation of Surface Modified Implants by HAp, Carbonated-HAp and Titanium Oxide

2007 ◽  
Vol 539-543 ◽  
pp. 687-691 ◽  
Author(s):  
Masazumi Okido ◽  
Ryoichi Ichino ◽  
Kotaro Kuroda
Keyword(s):  
Titanium Oxide ◽  
Titanium Implant ◽  
Titanium Implants ◽  
Contact Ratio ◽  
In Vivo Evaluation ◽  
Ft Ir ◽  
Mouse Osteoblast ◽  
Surface Modified

Hydroxyapatite (Ca10(PO4)6(OH)2, HAp), carbonated HAp and titanium oxide are of interest for bone-interfacing implant applications, because of their demonstrated osteoconductive properties. They were coated on the titanium implants and investigated the in vitro and in vivo performance. HAp coatings were performed by the thermal substrate method in aqueous solutions. Titanium oxide film was also formed on the titanium implants by gaseous oxidation, or by anodizing in the acidic solution. All the specimens covered with HAp, carbonated HAp or TiO2 (rutile or anatase). were characterized by XRD, EDX, FT-IR and SEM. In the in vitro testing, the mouse osteoblast-like cells (MC3T3-E1) were cultured on the coated and non-coated specimens for up to 30 days. Moreover, the osseointegration was evaluated from the rod specimens implanted in rats femoral for up to 8 weeks. In in vivo evaluations two weeks postimplantation, new bone formed on the coated and non-coated titanium rods in the cancellous bone and cortical bone, respectively. Bone-implant contact ratio, in order to evaluate of new bone formation, was significantly depended on the compound formed on the titanium implant.

Bone Formation by Human Oseoblasts on Implant Materials

1998 ◽  
Vol 4 (S2) ◽  
pp. 938-939
Author(s):  
G. Gronowicz ◽  
M. Ahmad
Keyword(s):  
In Vitro System ◽  
Implant Materials ◽  
Sequence Of Events ◽  
Ecm Proteins ◽  
Transmission Electron ◽  
Fluorescence Light ◽  
Primary Human Osteoblasts ◽  
Fetal Bovine

We have developed an in vitro system to study the process by which osteoblasts attach and secrete extracellular matrix (ECM) proteins that subsequently mineralize in response to implant materials. In vivo mineralization is characterized by the sequential expression by osteoblasts of specific ECM proteins that are capable of calcifying in an orderly manner. A similar sequence of events is shown to occur in this in vitro system. The extent and pattern of mineralization was visualized by fluorescence light microscopy and transmission electron microscopy (TEM), and was dependent on the substrate to which osteoblasts adhered.Materials and MethodsDisks (22 mm diameter) composed of Tivanium (Tiv) (Ti6AI4V, ASTM F-136) and Zimaloy (Zim)(CoCrMo, ASTM F-75), prepared identically to clinical orthopaedic implants, were supplied by Zimmer, Inc. Glass disks were used as controls. Osteoblast-like cells (Saos-2), derived from a human osteosarcoma, or primary human osteoblasts from a 41 year-old male patient were added to the disks in a-MEM medium with 10% fetal bovine serum,

scholarly journals New titanium implant surface – An in vitro and in vivo evaluation

2019 ◽  
Vol 30 (S19) ◽  
pp. 213-213
Author(s):  
Sergio Gehrke ◽  
Leticia Pèrez‐DÌaz ◽  
Patricia Mazon ◽  
Piedad N. de Aza
Keyword(s):  
Titanium Implant ◽  
Implant Surface ◽  
In Vivo Evaluation ◽  
Titanium Implant Surface

scholarly journals Lactoferrin Coating Improves the Antibacterial and Osteogenic Properties of Alkali-Treated Titanium with Nanonetwork Structures

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Derong Yin ◽  
Yonglong Hong ◽  
Luyuan Chen ◽  
Satoshi Komasa ◽  
Yuanyuan Yang ◽  
...  
Keyword(s):  
Dental Implant ◽  
Alkali Treatment ◽  
Pure Titanium ◽  
Antimicrobial Properties ◽  
Biological Properties ◽  
Bacterial Attachment ◽  
Physiological Concentration ◽  
Implant Materials

Titanium and its alloys are the main dental implant materials used at present. The biological properties of pure titanium can be further improved by surface treatment methods. Alkali treatment of pure titanium at room temperature can form nanonetwork structures (TNS) on the surface, which has better osteoinductive ability than pure titanium. However, TNS does not possess antimicrobial properties, and bacterial infection is one of the main reasons for the failure of dental implant therapy. Therefore, it was the focus of our research to endow TNS with certain antimicrobial properties on the premise of maintaining its osteoinductive ability. Because of its excellent broad-spectrum antimicrobial properties and because it promotes osteoblast-like cell growth, lactoferrin (LF) was considered a promising prospect as a surface biological treatment material. In this study, bovine LF of physiological concentration was successfully coated on the surface of TNS to form the TNS-LF composite material. Results from in vitro and in vivo experiments showed that TNS-LF had better osteoinductive ability than TNS. Bacterial attachment and biofilm formation were also significantly decreased on the surface of TNS-LF. Therefore, this study has provided an experimental basis for the development of osteoinduction-antimicrobial composite implant materials for dental applications.

scholarly journals In vitro cytotoxicity and surface topography evaluation of additive manufacturing titanium implant materials

2017 ◽  
Vol 28 (3) ◽  
Author(s):  
Jukka T. Tuomi ◽  
Roy V. Björkstrand ◽  
Mikael L. Pernu ◽  
Mika V. J. Salmi ◽  
Eero I. Huotilainen ◽  
...  
Keyword(s):  
Additive Manufacturing ◽  
Surface Topography ◽  
Titanium Implant ◽  
In Vitro Cytotoxicity ◽  
Implant Materials

Poly(2-hydroxyethyl methacrylate) sponges as implant materials: in vivo and in vitro evaluation of cellular invasion

Biomaterials ◽  
1993 ◽  
Vol 14 (1) ◽  
pp. 26-38 ◽  
Author(s):  
Traian V. Chirila ◽  
Ian J. Constable ◽  
Geoffrey J. Crawford ◽  
Sarojini Vijayasekaran ◽  
Dawn E. Thompson ◽  
...  
Keyword(s):  
Hydroxyethyl Methacrylate ◽  
In Vitro Evaluation ◽  
Cellular Invasion ◽  
Implant Materials

scholarly journals In-vivo Investigations of Hydroxyapatite/Co-polymeric Composites Coated Titanium Plate for Bone Regeneration

2021 ◽  
Vol 8 ◽  
Author(s):  
Weilong Diwu ◽  
Xin Dong ◽  
Omaima Nasif ◽  
Sulaiman Ali Alharbi ◽  
Jian Zhao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Chemical Nature ◽  
Bone Cells ◽  
Titanium Implant ◽  
Titanium Plate ◽  
Uv Visible ◽  
Xrd Techniques ◽  
Porous Morphology

A perfect mimic of human bone is very difficult. Still, the latest advancement in biomaterials makes it possible to design composite materials with morphologies merely the same as that of bone tissues. In the present work is the fabrication of selenium substituted Hydroxyapatite (HAP-Se) covered by lactic acid (LA)—Polyethylene glycol (PEG)—Aspartic acid (AS) composite with the loading of vincristine sulfate (VCR) drug (HAP-Se/LA-PEG-AS/VCR) for twin purposes of bone regenerations. The HAP-Se/LA-PEG-AS/VCR composite coated on titanium implant through electrophoretic deposition (EPD). The prepared composite characterized using FTIR, XRD techniques to rely on the composites' chemical nature and crystalline status. The morphology of the composite and the titanium plate with the composite coating was investigated by utilizing SEM, TEM instrument techniques, and it reveals the composite has porous morphology. The drug (VCR) load in HAP-Se/LA-PEG-AS and releasing nature were investigated through UV-Visible spectroscopy at the wavelength of 295 nm. In vitro study of SBF treatment shows excellent biocompatibility to form the HAP crystals. The viability against MG63 and toxicity against Saos- 2 cells have expressed the more exceptional biocompatibility in bone cells and toxicity with the cancer cells of prepared composites. The in-vivo study emphasizes prepared biomaterial suitable for implantation and helps accelerate bone regeneration on osteoporosis and osteosarcoma affected hard tissue.

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