Lactoferrin Coating Improves the Antibacterial and Osteogenic Properties of Alkali-Treated Titanium with Nanonetwork Structures

Titanium and its alloys are the main dental implant materials used at present. The biological properties of pure titanium can be further improved by surface treatment methods. Alkali treatment of pure titanium at room temperature can form nanonetwork structures (TNS) on the surface, which has better osteoinductive ability than pure titanium. However, TNS does not possess antimicrobial properties, and bacterial infection is one of the main reasons for the failure of dental implant therapy. Therefore, it was the focus of our research to endow TNS with certain antimicrobial properties on the premise of maintaining its osteoinductive ability. Because of its excellent broad-spectrum antimicrobial properties and because it promotes osteoblast-like cell growth, lactoferrin (LF) was considered a promising prospect as a surface biological treatment material. In this study, bovine LF of physiological concentration was successfully coated on the surface of TNS to form the TNS-LF composite material. Results from in vitro and in vivo experiments showed that TNS-LF had better osteoinductive ability than TNS. Bacterial attachment and biofilm formation were also significantly decreased on the surface of TNS-LF. Therefore, this study has provided an experimental basis for the development of osteoinduction-antimicrobial composite implant materials for dental applications.

Download Full-text

Studying Biomimetic Coated Niobium as an Alternative Dental Implant Material to Titanium (in vitro and in vivo study)

Baghdad Science Journal ◽

10.21123/bsj.15.3.253-261 ◽

2018 ◽

Vol 15 (3) ◽

pp. 253-261

Author(s):

Baghdad Science Journal

Keyword(s):

Dental Implant ◽

Body Fluid ◽

Pure Titanium ◽

Commercially Pure Titanium ◽

Removal Torque ◽

X Ray ◽

Commercially Pure ◽

Implant Material

Commercially pure titanium (cpTi) is widely used as dental implant material although it was found that titanium exhibited high modulus of elasticity and the lower corrosion tendency in oral environment. Niobium(Nb) was chosen for this study as an alternative to cpTi implant material due to its bioinert behavior and good elastic modulus and moderate cost in addition to corrosion resistance. This study was done to evaluate the effect of biomimetic coating on the surface properties of the commercially pure titanium and niobium implants by in vitro and in vivo experiments. The in vitro study was involved etching the samples of each material in HCl then soaking in 10M NaOH aqueous solution. These samples were then immersed in a 5 times concentrated simulated body fluid for 14 days. Scanning Electron Microscope, Energy Dispersive X-ray, and X-Ray Diffraction tests were done to analyze surface changes. The in vivo study was done by the implantation of screw-shaped implants (two from each material, uncoated and the other was biomimetically coated) in the tibias of New Zealand rabbits. After 2 and 4 weeks of healing period, 20 rabbits were sacrificed for each period. A removal torque was done for ten animals in each group, whereas the other ten were used for histological testing and histomorphometric analysis with optical microscope.The in vitro experiments showed that the use of 14 days immersion in a concentrated simulated body fluid produced a layer of calcium phosphate on metal surfaces. The removal torque values and new bone formation were increased significantly in Nb than Ti, in coated than uncoated screws, and in 4 weeks than 2 weeks healing periods. The Nb implants had better biomechanical and biological properties than the commercially pure titanium implants and can be used as an alternative dental implant.

Download Full-text

Silver Nanoparticles in Alveolar Bone Surgery Devices

Journal of Nanomaterials ◽

10.1155/2012/975842 ◽

2012 ◽

Vol 2012 ◽

pp. 1-12 ◽

Cited By ~ 28

Author(s):

Stefano Sivolella ◽

Edoardo Stellini ◽

Giulia Brunello ◽

Chiara Gardin ◽

Letizia Ferroni ◽

...

Keyword(s):

Silver Nanoparticles ◽

Dental Implant ◽

Animal Studies ◽

Alveolar Bone ◽

Antimicrobial Properties ◽

Ag Nps ◽

Augmentation Techniques ◽

Dental Implant Surgery

Silver (Ag) ions have well-known antimicrobial properties and have been applied as nanostrategies in many medical and surgical fields, including dentistry. The use of silver nanoparticles (Ag NPs) may be an option for reducing bacterial adhesion to dental implant surfaces and preventing biofilm formation, containing the risk of peri-implant infections. Modifying the structure or surface of bone grafts and membranes with Ag NPs may also prevent the risk of contamination and infection that are common when alveolar bone augmentation techniques are used. On the other hand, Ag NPs have revealed some toxic effects on cellsin vitroandin vivoin animal studies. In this setting, the aim of the present paper is to summarize the principle behind Ag NP-based devices and their clinical applications in alveolar bone and dental implant surgery.

Download Full-text

Impact of surface topography and coating on osteogenesis and bacterial attachment on titanium implants

Journal of Tissue Engineering ◽

10.1177/2041731418790694 ◽

2018 ◽

Vol 9 ◽

pp. 204173141879069 ◽

Cited By ~ 45

Author(s):

Laila Damiati ◽

Marcus G Eales ◽

Angela H Nobbs ◽

Bo Su ◽

Penelope M Tsimbouri ◽

...

Keyword(s):

Surface Topography ◽

Dental Implants ◽

Antimicrobial Properties ◽

Bacterial Attachment ◽

Titanium Implants ◽

Premature Failure ◽

Biological Environment ◽

The Impact

Titanium (Ti) plays a predominant role as the material of choice in orthopaedic and dental implants. Despite the majority of Ti implants having long-term success, premature failure due to unsuccessful osseointegration leading to aseptic loosening is still too common. Recently, surface topography modification and biological/non-biological coatings have been integrated into orthopaedic/dental implants in order to mimic the surrounding biological environment as well as reduce the inflammation/infection that may occur. In this review, we summarize the impact of various Ti coatings on cell behaviour both in vivo and in vitro. First, we focus on the Ti surface properties and their effects on osteogenesis and then on bacterial adhesion and viability. We conclude from the current literature that surface modification of Ti implants can be generated that offer both osteoinductive and antimicrobial properties.

Download Full-text

Influence of Chitosan or Keratin on Titanium Implant Surface - A Systematic Review

International Journal of Regenerative Medicine ◽

10.31487/j.rgm.2020.01.04 ◽

2020 ◽

pp. 1-12

Author(s):

Robert Love ◽

Eliza Ranjit ◽

Ajay Sharma ◽

Stephen Hamlet ◽

Roy George ◽

...

Keyword(s):

Systematic Review ◽

Dental Implant ◽

Science Studies ◽

Titanium Implant ◽

Biological Properties ◽

Implant Surface ◽

Chitosan Hydrogel ◽

Chitosan Hydrogels

Purpose: This systematic review was carried out to investigate the effects of keratin and chitosan hydrogel preparations on dental implant osseointegration. Materials and Methods: The electronic search was conducted on five databases: Scopus, EBSCOhost MEDLINE, EBSCOhost Dentistry and Oral Science, PubMed, and Web of Science. Studies that determined the in vitro or in vivo efficacy of keratin and chitosan hydrogel on osseointegration were included in the review. Results: Of the 760 studies initially gathered, nine met the inclusion criteria. These studies demonstrated that dental implants coated with keratin and chitosan hydrogels resulted in improved biological properties. It was also concluded that the inclusion of chitosan in keratin hydrogels improves the mechanical strength and helps increase durability through ameliorating degradation and swelling characters. Both the polymers increased bone-implant contact and new bone formation in animal models. Conclusion: This systematic review demonstrates that keratin and chitosan hydrogel, is effective in initiating osteogenesis, reinforcing the currently available evidence that these polymers could be a substrate in dental implant treatment.

Download Full-text

In vitro Behaviour of Alumina-Hydroxiapatite Composites Coatings

Revista de Chimie ◽

10.37358/rc.18.6.6336 ◽

2018 ◽

Vol 69 (6) ◽

pp. 1416-1418

Author(s):

Alexandru Szabo ◽

Ilare Bordeasu ◽

Ion Dragos Utu ◽

Ion Mitelea

Keyword(s):

Pure Titanium ◽

Titanium Substrate ◽

High Strength ◽

Biological Properties ◽

Commercially Pure Titanium ◽

Hvof Spraying ◽

Before And After ◽

Sbf Solution ◽

Oxygen Fuel

Hydroxyapatite (HA) is a very common material used for biomedical applications. Usually, in order to improve its poor mechanical properties is combined or coated with other high-strength materials.The present paper reports the manufacturing and the biocompatibility behaviour of two different biocomposite coatings consisting of alumina (Al2O3) and hydroxyapatite (HA) using the high velocity oxygen fuel (HVOF) spraying method which were deposited onto the surface of a commercially pure titanium substrate. The biological properties of the Al2O3-HA materials were evaluated by in vitro studies. The morphology of the coatings before and after their immersing in the simulated body fluid (SBF) solution was characterized by scanning electron microscopy (SEM). The results showed an important germination of the biologic hydroxyapatite crystallite on the surface of both coatings.

Download Full-text

Nanodrugs as a new approach in therapy of cardiovascular diseases and cancer with tumor-associated angiogenesis

Current Medicinal Chemistry ◽

10.2174/0929867328666201231121704 ◽

2020 ◽

Vol 28 ◽

Author(s):

Justyna Hajtuch ◽

Karolina Niska ◽

Iwona Inkielewicz-Stepniak

Keyword(s):

Cardiovascular Disease ◽

Cardiovascular Diseases ◽

Controlled Drug Release ◽

Biological Properties ◽

Comprehensive Information ◽

Conventional Drug ◽

Key Factor ◽

Functionalized Materials

Background: Cancer along with cardiovascular diseases are globally defined as leading causes of death. Importantly, some risk factors are common to these diseases. The process of angiogenesis and platelets aggregation are observed in cancer development and progression. In recent years, studies have been conducted on nanodrugs in these diseases that have provided important information on the biological and physicochemical properties of nanoparticles. Their attractive features are that they are made of biocompatible, well-characterized and easily functionalized materials. Unlike conventional drug delivery, sustained and controlled drug release can be obtained by using nanomaterials. Methods: In this article, we review the latest research to provide comprehensive information on nanoparticle-based drugs for the treatment of cancer, cardiovascular disease associated with abnormal haemostasis, and the inhibition of tumorassociated angiogenesis. Results: The results of the analysis of data based on nanoparticles with drugs confirm their improved pharmaceutical and biological properties, which gives promising antiplatelet, anticoagulant and antiangiogenic effects. Moreover, the review included in vitro, in vivo research and presented nanodrugs with chemotherapeutics approved by Food and Drug Administration. Conclusion: By the optimization of nanoparticles size and surface properties, nanotechnology are able to deliver drugs with enhanced bioavailability in treatment of cardiovascular disease, cancer and inhibition of cancer-related angiogenesis. Thus, nanotechnology can improve the therapeutic efficacy of the drug, but there is a need for a better understanding of the nanodrugs interaction in the human body, because this is a key factor in the success of potential nanotherapeutics.

Download Full-text

Tamarix articulata (T. articulata) - An Important Halophytic Medicinal Plant with Potential Pharmacological Properties

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190318120103 ◽

2019 ◽

Vol 20 (4) ◽

pp. 285-292 ◽

Cited By ~ 5

Author(s):

Abdullah M. Alnuqaydan ◽

Bilal Rah

Keyword(s):

Medicinal Plant ◽

Biological Activities ◽

Wide Spectrum ◽

Biological Properties ◽

In Vivo Model ◽

Drug Candidate ◽

Phytochemical Analysis ◽

Pharmacological Properties

Background:Tamarix Articulata (T. articulata), commonly known as Tamarisk or Athal in Arabic region, belongs to the Tamaricaece species. It is an important halophytic medicinal plant and a good source of polyphenolic phytochemical(s). In traditional medicines, T. articulata extract is commonly used, either singly or in combination with other plant extracts against different ailments since ancient times.Methods:Electronic database survey via Pubmed, Google Scholar, Researchgate, Scopus and Science Direct were used to review the scientific inputs until October 2018, by searching appropriate keywords. Literature related to pharmacological activities of T. articulata, Tamarix species, phytochemical analysis of T. articulata, biological activities of T. articulata extracts. All of these terms were used to search the scientific literature associated with T. articulata; the dosage of extract, route of administration, extract type, and in-vitro and in-vivo model.Results:Numerous reports revealed that T. articulata contains a wide spectrum of phytochemical(s), which enables it to have a wide window of biological properties. Owing to the presence of high content of phytochemical compounds like polyphenolics and flavonoids, T. articulata is a potential source of antioxidant, anti-inflammatory and antiproliferative properties. In view of these pharmacological properties, T. articulata could be a potential drug candidate to treat various clinical conditions including cancer in the near future.Conclusion:In this review, the spectrum of phytochemical(s) has been summarized for their pharmacological properties and the mechanisms of action, and the possible potential therapeutic applications of this plant against various diseases discussed.

Download Full-text

99mTc Labelling Strategies for the Development of Potential Nitroimidazolic Hypoxia Imaging Agents

Inorganics ◽

10.3390/inorganics7110128 ◽

2019 ◽

Vol 7 (11) ◽

pp. 128 ◽

Cited By ~ 3

Author(s):

Giglio ◽

Rey

Keyword(s):

Rational Design ◽

Biological Properties ◽

Fine Tuning ◽

Oxidation States ◽

Hypoxia Imaging ◽

Biological Target ◽

99Mtc Radiopharmaceuticals ◽

99Mtc Labelling

Technetium-99m has a rich coordination chemistry that offers many possibilities in terms of oxidation states and donor atom sets. Modifications in the structure of the technetium complexes could be very useful for fine tuning the physicochemical and biological properties of potential 99mTc radiopharmaceuticals. However, systematic study of the influence of the labelling strategy on the “in vitro” and “in vivo” behaviour is necessary for a rational design of radiopharmaceuticals. Herein we present a review of the influence of the Tc complexes’ molecular structure on the biodistribution and the interaction with the biological target of potential nitroimidazolic hypoxia imaging radiopharmaceuticals presented in the literature from 2010 to the present. Comparison with the gold standard [18F]Fluoromisonidazole (FMISO) is also presented.

Download Full-text