One of the most important aspects of endemic populations conservation of modern honeybee breeds of is study in the field of drones morphological features identification in the apiaries, as the assessment of Queen bees’ purity breed and the potential for recovery of the populations. In this regard, studies of the honeybee drones’ breed in the Chuvash Republic (Chuvashia) are relevant and have scientific and prac-tical value. The raw material was a sample collection of drones (126000 pcs. from 4200 of the bee fami-lies) from 21 districts, which are covering all natural honey gathering zones of the Chuvash Republic: forest-steppe, forest and steppe. Three characteristics are identified: colour of the hairs on a scale of Gotze, cubital index and the length of the proboscis. The binocular microscope MBS-10 was used in the work. In the process of research, biogenetic potential of the Chuvash population of Central Russian breed (Apis mellifera mellifera) is established, in the conditions of hybridization, with the observed trend of an annual increase. Five subpopulation structures or administrative districts (Morgaushsky, Kras-noarmeysky, Krasnochetaysky, Shumerlinsky and Batyrevsky) are registered, where, the territory of "pure" breeding is stored and gradually created thanks to the selection-breeding works. The obtained re-sults prove the effectiveness of implemented local and regional programs and activities of breeding and distribution of Central Russian breed bees in the Chuvash Republic.