THE GECHEPSIN FORTIFIED SETTLEMENT: EXPERIENCE OF COMPREHENSIVE EXCAVATIONS

Несмотря на очевидный прогресс в наших представлениях о системе расселения восточной периферии азиатского Боспора, круг известных памятников совсем невелик, а об их хронологии можно судить только на основании подъемного материала. Комплексные исследования на городище Гечепсин позволили выявить два строительных горизонта, датируемых керамическими материалами в пределах II в. до н. э. - II в. н. э. и охарактеризовать особенности топографии (фортификацию, коммуникации и т. п.). Исследованная кладка сооружения фортификационной системы крепости была сооружена на культурном слое эпохи позднего эллинизма и просуществовала не более века. Выявленные на площадке городища постройки возведены, судя по всему, в античных традициях сырцово-каменной архитектуры. В конце I тыс. до н. э. - нач. I тыс. н. э. в районе городища, согласно спорово-пыльцевому анализу, были широко распространены степные злаково-разнотравные растительные сообщества. Присутствие пыльцы водных растений в верхней части профиля отражает, вероятнее всего, использование речной глины для возведения сырцовых конструкций. Despite obvious progress in developing our concepts on the settlement system in the eastern periphery of Asian Bosporus, the group of the known sites is not that large, while their chronology can be determined only with the use of surface finds. Comprehensive excavations at the Gechepsin fortified settlement made it possible to identify two construction horizons dated by ceramic finds to 2 century BC -2 century AD and characterize specifics of topography (fortification, communications, etc.). The examined masonry of the fortress fortification construction was built in the occupation layer of the Late Hellenic period and did not function more than half a century. Constructions identified at the fortified settlement were, most likely, built in Ancient Greek traditions of adobe-stone architecture. At the end of I mill. BC - early I mill. AD steppe gramineous-mixed grass plant associations were widespread in the area around the settlement as evidenced by pollen analysis. Presence of water plant pollen in the upper part of the profile, most likely, reflects use of river clay for building adobe constructions.

PLANT POLLINATION CALENDAR AND ITS IMPORTANCE FOR OPTIMIZATION OF THE TREATMENT AND REHABILITATION OF PATIENTS WITH SENSITIZATION TO PLANT POLLEN ON THE SOUTHERN COAST OF THE CRIMEA

Air-aerosol pollen of plants can influence the results of climatotherapy and elimination therapy of respiratory allergic diseases. The diversity of the flora, the poorly studied prevalence and etiology of pollinosis on the Southern coast of the Crimea determine the need for aeropalinological studies of this region to optimize the treatment and rehabilitation of patients with respiratory sensitization. Objective To determine the content of plant pollen in the air, to develop a calendar of palination of plants in the Yalta resort, to identify periods that are potentially unfavorable for the stay of patients with pollen sensitization on the Southern coast of the Crimea. Material and methods Plant pollen in the air was determined gravimetrically. The taxonomic affiliation was identified and the amount of pollen deposited on the glass-traps in the coastal and foothill areas of Yalta city was calculated. The data obtained were averaged over the city as a whole. Results In 2011-2013, 19 pollen taxa were identified in the air of the resort, including 10 with sensitizing properties. Of these, cypress pollen accounted for 49.7% of the average annual amount, deciduous trees with allergenic pollen 4.7%, cereals and weeds 1.6% each. 79.0% of pollen was recorded in spring, 7.7% in summer, 8.6% in autumn, 4.7% in winter. In the air of the coastal area of the city, 2.2 times less pollen was detected than in the air of the foothill. Three waves of plants pollination were revealed: winter-spring pollination of trees and shrubs, late spring pollination of cereals, summer-autumn pollination of weeds. The calendar of pollination of plants of the Yalta resort has been developed. Conclusion The greatest risk of sensitization and exacerbation of respiratory allergies in the Yalta resort is created by cypress pollen in March April. The most favorable for the treatment and rehabilitation of patients with respiratory allergies is the coastal area of the resort. The developed plant pollination calendar allows predicting unfavorable aeropalinological periods, optimizing the treatment and rehabilitation of patients with pollen sensitization at the Yalta resort.

Use of 3D Laser Microscopy to Determine Plant Pollen

Pollen analysis as a part of palynology deals with the morphological determination of pollen and spores. Different technologies with different resolutions varying from simple light microscopy to highly elaborate electron microscopy are used for the examination, depending on the area of application (e.g. sedimentology, melissopalynology, forensic palynology, etc.). To answer the question of whether laser scanning microscopy (LSM) can replace scanning electron microscopy (SEM) for the determination of pollen species, 168 species were examined using LSM. It was concluded that LSM is both efficient and easy to handle. After preparing the fresh pollen, a 3D laser scan takes 5-10 minutes and unlike using SEM, the pollen does not have to be sputtered or processed. The 3D scans can be measured quickly and easily with the integrated software and there were no observable artifacts. At magnifications up to 8545x, the image quality is comparable to that of a sputtered SEM sample whereas at higher magnifications, the SEM method is superior. Overall, pollen display by LSM is much less time consuming and more cost effective than with the SEM method.

Honey Bees Can Taste Amino and Fatty Acids in Pollen, but Not Sterols

The nutritional composition of food is often complex as resources contain a plethora of different chemical compounds, some of them more, some less meaningful to consumers. Plant pollen, a major food source for bees, is of particular importance as it comprises nearly all macro- and micronutrients required by bees for successful development and reproduction. However, perceiving and evaluating all nutrients may be tedious and impair quick foraging decisions. It is therefore likely that nutrient perception is restricted to specific nutrients or nutrient groups. To better understand the role of taste in pollen quality assessment by bees we investigated nutrient perception in the Western honey bee, Apis mellifera. We tested if the bees were able to perceive concentration differences in amino acids, fatty acids, and sterols, three highly important nutrient groups in pollen, via antennal reception. By means of proboscis extension response (PER) experiments with chemotactile stimulation, we could show that honey bees can distinguish between pollen differing in amino and fatty acid concentration, but not in sterol concentration. Bees were also not able to perceive sterols when presented alone. Our finding suggests that assessment of pollen protein and lipid content is prioritized over sterol content.

Bee Pollen: Current Status and Therapeutic Potential

Bee pollen is a combination of plant pollen and honeybee secretions and nectar. The Bible and ancient Egyptian texts are documented proof of its use in public health. It is considered a gold mine of nutrition due to its active components that have significant health and medicinal properties. Bee pollen contains bioactive compounds including proteins, amino acids, lipids, carbohydrates, minerals, vitamins, and polyphenols. The vital components of bee pollen enhance different bodily functions and offer protection against many diseases. It is generally marketed as a functional food with affordable and inexpensive prices with promising future industrial potentials. This review highlights the dietary properties of bee pollen and its influence on human health, and its applications in the food industry.

Plant Pollen Grains: A Move Towards Green Drug and Vaccine Delivery Systems

Pollen grains and plant spores have emerged as innovative biomaterials for various applications such as drug/vaccine delivery, catalyst support, and the removal of heavy metals. The natural microcapsules comprising spore shells and pollen grain are designed for protecting the genetic materials of plants from exterior impairments. Two layers make up the shell, the outer layer (exine) that comprised largely of sporopollenin, and the inner layer (intine) that built chiefly of cellulose. These microcapsule shells, namely hollow sporopollenin exine capsules have some salient features such as homogeneity in size, non-toxic nature, resilience to both alkalis and acids, and the potential to withstand at elevated temperatures; they have displayed promising potential for the microencapsulation and the controlled drug delivery/release. The important attribute of mucoadhesion to intestinal tissues can prolong the interaction of sporopollenin with the intestinal mucosa directing to an augmented effectiveness of nutraceutical or drug delivery. Here, current trends and prospects related to the application of plant pollen grains for the delivery of vaccines and drugs and vaccine are discussed. "Image missing"

Changes of microorganism composition in fresh and stored bee pollen from Southern Germany

Analysis of plant pollen can provide valuable insights into the existing spectrum of microorganisms in the environment. When harvesting bee-collected pollen as a dietary supplement for human consumption, timely preservation of the freshly collected pollen is fundamental for product quality. Environmental microorganisms contained in freshly collected pollen can lead to spoilage by degradation of pollen components. In this study, freshly collected bee pollen was sampled at different locations and stored under various storage conditions to examine the hypothesis that storage conditions may have an effect on the composition of microorganisms in pollen samples. The samples were analyzed using 16S and 18S amplicon sequencing and characterized by palynological analysis. Interestingly, the bacterial communities between pollen samples from different locations varied only slightly, whereas for fungal community compositions, this effect was substantially increased. Further, we noticed that fungal communities in pollen are particularly sensitive to storage conditions. The fungal genera proportion Cladosporium and Mycosphaerella decreased, while Zygosaccharomyces and Aspergillus increased during storage. Aspergillus and Zygosaccharomyces fractions increased during storage at 30 °C, which could negatively impact the pollen quality if it is used as a dietary supplement.

Amplicon Sequencing of Variable 16S rRNA from Bacteria and ITS2 Regions from Fungi and Plants, Reveals Honeybee Susceptibility to Diseases Results from Their Forage Availability under Anthropogenic Landscapes

European Apis mellifera and Asian Apis cerana honeybees are essential crop pollinators. Microbiome studies can provide complex information on health and fitness of these insects in relation to environmental changes, and plant availability. Amplicon sequencing of variable regions of the 16S rRNA from bacteria and the internally transcribed spacer (ITS) regions from fungi and plants allow identification of the metabiome. These methods provide a tool for monitoring otherwise uncultured microbes isolated from the gut of the honeybees. They also help monitor the composition of the gut fungi and, intriguingly, pollen collected by the insect. Here, we present data from amplicon sequencing of the 16S rRNA from bacteria and ITS2 regions from fungi and plants derived from honeybees collected at various time points from anthropogenic landscapes such as urban areas in Poland, UK, Spain, Greece, and Thailand. We have analysed microbial content of honeybee intestine as well as fungi and pollens. Furthermore, isolated DNA was used as the template for screening pathogens: Nosema apis, N. ceranae, N. bombi, tracheal mite (Acarapis woodi), any organism in the parasitic order Trypanosomatida, including Crithidia spp. (i.e., Crithidia mellificae), neogregarines including Mattesia and Apicystis spp. (i.e., Apicistis bombi). We conclude that differences between samples were mainly influenced by the bacteria, plant pollen and fungi, respectively. Moreover, honeybees feeding on a sugar based diet were more prone to fungal pathogens (Nosema ceranae) and neogregarines. In most samples Nosema sp. and neogregarines parasitized the host bee at the same time. A higher load of fungi, and bacteria groups such as Firmicutes (Lactobacillus); γ-proteobacteria, Neisseriaceae, and other unidentified bacteria was observed for Nosema ceranae and neogregarine infected honeybees. Healthy honeybees had a higher load of plant pollen, and bacteria groups such as: Orbales, Gilliamella, Snodgrassella, and Enterobacteriaceae. Finally, the period when honeybees switch to the winter generation (longer-lived forager honeybees) is the most sensitive to diet perturbations, and hence pathogen attack, for the whole beekeeping season. It is possible that evolutionary adaptation of bees fails to benefit them in the modern anthropomorphised environment.