CRISPR-Cas9-Mediated Editing of the CYP82E4-Nicotine N-Demethylase (nnd) Gene in Tobacco Protoplasts

Ankita Shrestha; Ahamed Khan; Nrisingha Dey

doi:10.4172/2329-9029.1000230

CRISPR-Cas9-Mediated Editing of the CYP82E4-Nicotine N-Demethylase (nnd) Gene in Tobacco Protoplasts

Journal of Plant Biochemistry & Physiology ◽

10.4172/2329-9029.1000230 ◽

2019 ◽

Vol 07 (01) ◽

Author(s):

Ankita Shrestha ◽

Ahamed Khan ◽

Nrisingha Dey

Keyword(s):

Tobacco Protoplasts

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Faculty Opinions recommendation of The thylakoid delta pH/delta psi are not required for the initial stages of Tat-dependent protein transport in tobacco protoplasts.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1028592.341186 ◽

2005 ◽

Author(s):

Steven Theg

Keyword(s):

Protein Transport ◽

Tobacco Protoplasts ◽

Dependent Protein

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Glucose and Glycine Metabolism in Regenerating Tobacco Protoplasts

PLANT PHYSIOLOGY ◽

10.1104/pp.77.2.374 ◽

1985 ◽

Vol 77 (2) ◽

pp. 374-378 ◽

Cited By ~ 14

Author(s):

Michal Neeman ◽

Dvora Aviv ◽

Hadassa Degani ◽

Esra Galun

Keyword(s):

Tobacco Protoplasts ◽

Glycine Metabolism

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Auxin Effect on the Transmembrane Potential Difference of Wild-Type and Mutant Tobacco Protoplasts Exhibiting a Differential Sensitiity to Auxin

PLANT PHYSIOLOGY ◽

10.1104/pp.83.4.801 ◽

1987 ◽

Vol 83 (4) ◽

pp. 801-804 ◽

Cited By ~ 61

Author(s):

Genevieve Ephritikhine ◽

Helene Barbier-Brygoo ◽

Jean-François Muller ◽

Jean Guern

Keyword(s):

Transmembrane Potential ◽

Potential Difference ◽

Wild Type ◽

Tobacco Protoplasts ◽

Transmembrane Potential Difference ◽

Auxin Effect

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A Reproducible Method for the Introduction of Nucleic Acids in Tobacco Protoplasts

Protoplasts 1983 ◽

10.1007/978-3-0348-6556-2_65 ◽

1983 ◽

pp. 140-141

Author(s):

F. A. Krens ◽

G. J. Wullems ◽

R. A. Schilperoort

Keyword(s):

Nucleic Acids ◽

Tobacco Protoplasts ◽

Reproducible Method

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Auxin inducibility and developmental expression of axi 1: a gene directing auxin independent growth in tobacco protoplasts

The EMBO Journal ◽

10.1038/sj.emboj.7591711b ◽

1999 ◽

Vol 18 (10) ◽

pp. 2908-2908

Keyword(s):

Developmental Expression ◽

Tobacco Protoplasts

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Salt stress-induced programmed cell death in tobacco protoplasts is mediated by reactive oxygen species and mitochondrial permeability transition pore status

Journal of Plant Physiology ◽

10.1016/j.jplph.2005.06.016 ◽

2006 ◽

Vol 163 (7) ◽

pp. 731-739 ◽

Cited By ~ 69

Author(s):

Jiusheng Lin ◽

Yuan Wang ◽

Genxuan Wang

Keyword(s):

Reactive Oxygen Species ◽

Cell Death ◽

Salt Stress ◽

Mitochondrial Permeability Transition Pore ◽

Mitochondrial Permeability Transition ◽

Permeability Transition Pore ◽

Permeability Transition ◽

Oxygen Species ◽

Tobacco Protoplasts ◽

Mitochondrial Permeability

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Evidence that nucleocapsid disassembly and a later step in virus replication are inhibited in transgenic tobacco protoplasts expressing TMV coat protein

Virology ◽

10.1016/0042-6822(89)90143-8 ◽

1989 ◽

Vol 172 (1) ◽

pp. 370-373 ◽

Cited By ~ 40

Author(s):

Jane K. Osbourn ◽

John W. Watts ◽

Roger N. Beachy ◽

T.Michael A. Wilson

Keyword(s):

Coat Protein ◽

Transgenic Tobacco ◽

Virus Replication ◽

Tobacco Protoplasts

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Transient Expression Assays in Tobacco Protoplasts

Methods in Molecular Biology - Jasmonate Signaling ◽

10.1007/978-1-62703-414-2_18 ◽

2013 ◽

pp. 227-239 ◽

Cited By ~ 24

Author(s):

Robin Vanden Bossche ◽

Brecht Demedts ◽

Rudy Vanderhaeghen ◽

Alain Goossens

Keyword(s):

Transient Expression ◽

Tobacco Protoplasts

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Ultrastructural Observations of Cell Wall Regeneration Around Isolated Tobacco Protoplasts

Journal of Cell Science ◽

10.1242/jcs.14.2.439 ◽

1974 ◽

Vol 14 (2) ◽

pp. 439-449

Author(s):

J. BURGESS ◽

E. N. FLEMING

Keyword(s):

Electron Microscopy ◽

Heavy Metal ◽

Endoplasmic Reticulum ◽

Cell Wall ◽

Cell Wall Regeneration ◽

Membrane Systems ◽

Tobacco Protoplasts ◽

Wall Formation ◽

Wall Regeneration ◽

Wall Growth

The process of cell wall regeneration around cultured protoplasts isolated from tobacco mesophyll has been examined by electron microscopy. The initially formed wall contains 2 components which stain with conventional heavy metal stains. The first consists of un-branched fibres, at first oriented at right angles to the plasmalemma surface. As wall growth proceeds the fibres lengthen and assume an orientation parallel to the plasmalemma. It seems probable that this component is cellulose. The second component of the wall is more amorphous and more densely stained. It is most frequently seen in situations where leaching of materials into the medium would be expected to be minimal. The endoplasmic reticulum and the plasmalemma are the only membrane systems which appear to contribute towards wall formation. No pattern of structure has been detected to explain the orientation or method of synthesis of the microfibrillar part of the wall.

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