scholarly journals Destruction of <i>Escherichia coli</i> and Broad-Host-Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions

2012 ◽  
Vol 02 (01) ◽  
pp. 1-7 ◽  
Author(s):  
Kyle L. Asfahl ◽  
Mary C. Savin
Keyword(s):  
Escherichia Coli ◽  
Host Range ◽  
Plasmid Dna ◽  
Field Conditions ◽  
Treated Wastewater ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Ozone Disinfection

Multiple mechanisms for expression of incompatibility by broad-host-range plasmid RK2

1982 ◽  
Vol 152 (3) ◽  
pp. 1078-1090
Author(s):  
R Meyer ◽  
M Hinds
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Plasmid Dna ◽  
Plasmid Vector ◽  
Broad Host Range ◽  
Plasmid Maintenance ◽  
Broad Host Range Plasmid ◽  
Expression Of Genes ◽  
Plasmid Partitioning ◽  
Plasmid Rk2

By cloning fragments of plasmid DNA, we have shown that RK2 expresses incompatibility by more than one mechanism. One previously identified (R. J. Meyer, Mol. Gen, Genet. 177:155--161, 1979; Thomas et al., Mol. Gen. Genet. 181:1--7, 1981) determinant for incompatibility is linked to the origin of plasmid DNA replication. When cloned into a plasmid vector, this determinant prevents the stable inheritance of a coresident RK2. However, susceptibility to this mechanism of incompatibility requires an active RK2 replicon and is abolished if another replicator is provided. We have also cloned a second incompatibility determinant, encoded within the 54.1- to 56.4-kilobase region of RK2 DNA, which we call IncP-1(II). An RK2 derivative remains sensitive to IncP-1(II), even when it is not replicating by means of the RK2 replicon. The 54.1- to 56.4-kilobase DNA does not confer susceptibility to the IncP-1(II) mechanism, nor does it encode a detectable system for efficient plasmid partitioning. The incompatibility may be related to the expression of genes mapping in the 54.1- to 56.4-kilobase region, which are required for plasmid maintenance and suppression of plasmid-encoded killing functions.

scholarly journals Natural Transformation of Plasmid DNA in Escherichia coli in Sterilized Soil Column

1970 ◽  
Vol 25 (1) ◽  
pp. 49-52
Author(s):  
M Mahabub-Uz-Zaman ◽  
Zia Uddin Ahmed
Keyword(s):  
Escherichia Coli ◽  
Plasmid Dna ◽  
Natural Transformation ◽  
Soil Column ◽  
Limiting Factor ◽  
Broad Host Range ◽  
E Coli ◽  
Competent Cells ◽  
Broad Host Range Plasmid ◽  
Plasmid Puc18

The present study was carried out to assess transformability of natural and laboratory strains of Escherichia coli by plasmid DNA under different transformation conditions in sterilized soil column. Transformation experiments were carried out in laboratory conditions and in sterile soil columns with CaCl2-treated competent cells and non-competent cells at log phase and stationary phase of growth using the broad host range plasmid pUC18. In soil column experiments, transformants were obtained after CaCl2 induced competence in both E. coli K12 DH5α and strain BM09 in the frequency of 10-8 to 10-9. In natural transformation assays, transformants appeared only in log phase cells of strain DH5α at a lower frequency (5.0 x 10-9), and in CaCl2-competent BM09 cells, but not in fresh cells. Thus the major limiting factor for natural transformation in environmental E. coli in soil column is probably the absence of a competent state. The significance of this finding has been discussed with respect to generally observed lower antibiotic resistance in environmental E. coli isolates from aquatic sources. Keywords: Natural transformation; Plasmid DNA; Escherichia coli; Competent stateDOI: http://dx.doi.org/10.3329/bjm.v25i1.4856 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 49-52

ParE toxin encoded by the broad-host-range plasmid RK2 is an inhibitor of Escherichia coli gyrase

2002 ◽  
Vol 44 (4) ◽  
pp. 971-979 ◽  
Author(s):  
Yong Jiang ◽  
Joe Pogliano ◽  
Donald R. Helinski ◽  
Igor Konieczny
Keyword(s):  
Escherichia Coli ◽  
Host Range ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Plasmid Rk2

scholarly journals The R1162 Mob Proteins Can Promote Conjugative Transfer from Cryptic Origins in the Bacterial Chromosome

2008 ◽  
Vol 191 (5) ◽  
pp. 1574-1580 ◽  
Author(s):  
Richard Meyer
Keyword(s):  
Escherichia Coli ◽  
Host Range ◽  
Active Site ◽  
Bacterial Chromosome ◽  
Broad Host Range ◽  
Conjugative Transfer ◽  
Chromosomal Dna ◽  
Bacterial Dna ◽  
Pectobacterium Atrosepticum ◽  
Broad Host Range Plasmid

ABSTRACT The mobilization proteins of the broad-host-range plasmid R1162 can initiate conjugative transfer of a plasmid from a 19-bp locus that is partially degenerate in sequence. Such loci are likely to appear by chance in the bacterial chromosome and could act as cryptic sites for transfer of chromosomal DNA when R1162 is present. The R1162-dependent transfer of chromosomal DNA, initiated from one such potential site in Pectobacterium atrosepticum, is shown here. A second active site was identified in Escherichia coli, where it is also shown that large amounts of DNA are transferred. This transfer probably reflects the combined activity of the multiple cryptic origins in the chromosome. Transfer of chromosomal DNA due to the presence of a plasmid in the cytoplasm describes a previously unrecognized potential for the exchange of bacterial DNA.

scholarly journals Expanding the toolbox of broad host-range transcriptional terminators for Proteobacteria through metagenomics

2018 ◽  
Author(s):  
Vanesa Amarelle ◽  
Ananda Sanches-Medeiros ◽  
Rafael Silva-Rocha ◽  
María-Eugenia Guazzaroni
Keyword(s):  
Escherichia Coli ◽  
Synthetic Biology ◽  
Pseudomonas Putida ◽  
Host Range ◽  
Broad Host Range ◽  
Functional Metagenomics ◽  
Alternative Hosts ◽  
E Coli ◽  
Broad Host Range Plasmid

AbstractAs the field of synthetic biology moves towards the utilization of novel bacterial chassis, there is a growing need for biological parts with enhanced performance in a wide number of hosts. Is not unusual that biological parts (such as promoters and terminators), initially characterized in the model bacteria Escherichia coli, do not perform well when implemented in alternative hosts, such as Pseudomonas, therefore limiting the construction of synthetic circuits in industrially relevant bacteria. In order to address this limitation, we present here the mining of transcriptional terminators through functional metagenomics to identify novel parts with broad host-range activity. Using a GFP-based terminator trap strategy and a broad host-range plasmid, we identified 20 clones with potential terminator activity in Pseudomonas putida. Further characterization allowed the identification of 4 unique sequences between 58 bp and 181 bp long that efficiently terminates transcription in P. putida, E. coli, Burkholderia phymatum and two Pseudomonas strains isolated from Antarctica. Therefore, this work presents a new set of biological parts useful for the engineering of synthetic circuits in Proteobacteria.

scholarly journals Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells.

1982 ◽  
Vol 79 (6) ◽  
pp. 1935-1939 ◽  
Author(s):  
D. H. Figurski ◽  
R. F. Pohlman ◽  
D. H. Bechhofer ◽  
A. S. Prince ◽  
C. A. Kelton
Keyword(s):  
Escherichia Coli ◽  
Host Range ◽  
Host Cells ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Plasmid Rk2
Sign in / Sign up

Export Citation Format

Share Document