scholarly journals Switchgrass (Panicum virgatum L.) Intraspecific Variation and Thermotolerance Classification Using in Vitro Seed Germination Assay

2011 ◽  
Vol 02 (02) ◽  
pp. 134-147 ◽  
Author(s):  
Ramdeo Seepaul ◽  
Bisoondat Macoon ◽  
K. Raja Reddy ◽  
Brian Baldwin
Keyword(s):  
Seed Germination ◽  
Intraspecific Variation ◽  
Panicum Virgatum ◽  
Germination Assay ◽  
Panicum Virgatum L ◽  
Seed Germination Assay

Arabidopsis Seed Germination Assay with Gibberellic Acid

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (22) ◽  
Author(s):  
Chunmei Zhong ◽  
Hao Xu ◽  
Siting Ye ◽  
Shengchun Zhang ◽  
Xiaojing Wang
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Arabidopsis Seed ◽  
Germination Assay ◽  
Seed Germination Assay

Seed Germination Assay of Hemisdesmus Indicus Ethanolic Root Extract An Invitro Analysis

2019 ◽  
Vol 10 (11) ◽  
pp. 3532
Author(s):  
T Nandhini ◽  
T. Lakshmi ◽  
S. Rajeshkumar ◽  
Anitha Roy ◽  
RV. Geetha
Keyword(s):  
Seed Germination ◽  
Root Extract ◽  
Germination Assay ◽  
Seed Germination Assay

scholarly journals Determination of swithgrass (Panicum virgatum L.) seeds quality

2021 ◽  
Author(s):  
V. A. Doronin ◽  
Yu. A. Kravchenko ◽  
V. V. Dryha ◽  
V. V. Doronin ◽  
H. S. Honcharuk
Keyword(s):  
Seed Germination ◽  
Panicum Virgatum ◽  
Production Test ◽  
Third Way ◽  
Cool Period ◽  
Germination Energy ◽  
Dormancy Period ◽  
Panicum Virgatum L

Purpose. Developing a method for determination of the laboratory seed germination that could reduce the biological dormancy period and, accordingly, increase the intensity of germination. Methods. Laboratory, measuring and weighing, mathematical and statistical. Results. Cooling switchgrass seeds at a temperature of 10°C for 7 days on average for three years did not lead to a decrease in germination energy and germination compared to cooling for 14 days. These indexes were almost the same and amounted to 74 and 76%, 73 and 75%, respectively. There were no significant deviations in germination energy and seed germination over the years of research as affected by duration of the cool period. The production test of the developed method, carried out in the accredited control and measuring laboratory, confirmed the obtained in the laboratory results. Conclusions. Seed germination by an improved method, when pre-cooling is carried out for 7 instead of 14 days and counting of germinated seeds on 15th day instead of 20th, has reduced the time to determine germination by 13 days without reducing the quality of analysis. It is advisable to determine the 1000-seed weight in one of three ways, but the most accurate is the third way, i.e. counting the seeds in 10 repetitions.

Bovine serum albumin mediated decrease in silver nanoparticle phytotoxicity: root elongation and seed germination assay

2012 ◽  
Vol 94 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Aswathy Ravindran ◽  
T.C. Prathna ◽  
Vinod Kumar Verma ◽  
N. Chandrasekaran ◽  
Amitava Mukherjee
Keyword(s):  
Seed Germination ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silver Nanoparticle ◽  
Bovine Serum ◽  
Root Elongation ◽  
Germination Assay ◽  
Seed Germination Assay

scholarly journals Evaluation of Switchgrass Genotypes for Cold-Tolerant Seed Germination from Native Populations in the Northeast USA

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 394
Author(s):  
Mayton ◽  
Amirkhani ◽  
Loos ◽  
Crawford ◽  
Crawford ◽  
...  
Keyword(s):  
Seed Germination ◽  
Panicum Virgatum ◽  
Percentage Germination ◽  
Original Population ◽  
Commercial Cultivar ◽  
Panicum Virgatum L ◽  
Native Populations ◽  
Cold Tolerant ◽  
Northeast Usa ◽  
Primary Focus

The focus of this research was to evaluate genotypes for cold-tolerant germination from wild switchgrass (Panicum virgatum L.) populations collected in the Northeast USA. Switchgrass nurseries were established in 2008 and 2009 with seed collected from native stands of switchgrass in the Northeast USA between 1991 and 2008. Switchgrass seed harvested from individual genotypes was evaluated for cold-tolerant germination in a series of laboratory experiments. Germination assays of seed of 13 switchgrass genotypes harvested in the fall of 2016 are the primary focus of this reported research. The selected genotypes were evaluated for cold-tolerant seed germination in three experiments, during the spring of 2017, fall of 2017 and spring of 2018, (with and without stratification) using a 10/15 °C regime with a 12 h photoperiod. Germination tests showed that several genotypes had significantly higher percentage germination as well as faster germination rates expressed as T50 (number of days required to reach 50% maximum germination) when compared to Cave-in-Rock, a moderately sensitive cold-tolerant commercial cultivar established in the original switchgrass nursery as a control. A final germination test was conducted to compare seed from the original population (no selection cycle 0), with one of the top performing cold-tolerant germination genotypes, and a commercial cultivar, ‘Espresso’, developed for low seed dormancy and low temperature germination. In this test, the selected genotype had significantly higher percentage germination in the stratified treatment and was not significantly different than Espresso in the non-stratified test. These data indicate successful selection for cold-tolerant germination in switchgrass genotypes from native germplasm collected in the Northeast USA.

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