scholarly journals Determination of swithgrass (Panicum virgatum L.) seeds quality

Author(s):  
V. A. Doronin ◽  
Yu. A. Kravchenko ◽  
V. V. Dryha ◽  
V. V. Doronin ◽  
H. S. Honcharuk

Purpose. Developing a method for determination of the laboratory seed germination that could reduce the biological dormancy period and, accordingly, increase the intensity of germination. Methods. Laboratory, measuring and weighing, mathematical and statistical. Results. Cooling switchgrass seeds at a temperature of 10°C for 7 days on average for three years did not lead to a decrease in germination energy and germination compared to cooling for 14 days. These indexes were almost the same and amounted to 74 and 76%, 73 and 75%, respectively. There were no significant deviations in germination energy and seed germination over the years of research as affected by duration of the cool period. The production test of the developed method, carried out in the accredited control and measuring laboratory, confirmed the obtained in the laboratory results. Conclusions. Seed germination by an improved method, when pre-cooling is carried out for 7 instead of 14 days and counting of germinated seeds on 15th day instead of 20th, has reduced the time to determine germination by 13 days without reducing the quality of analysis. It is advisable to determine the 1000-seed weight in one of three ways, but the most accurate is the third way, i.e. counting the seeds in 10 repetitions.

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
M Savchuk ◽  
◽  
O Sorochinska ◽  
A Tsiko ◽  
O Pilipchuk ◽  
...  

The paper presents results of the study phytotoxicity level of soil that are contaminated due to the operation of the railway. The urgency of the work is determined by the growth of man-made load on the environment by rail. The aim of the presented work was to assess the phytotoxicity of soils near railway tracks using a bioassay for watercress to determine the level of their anthropogenic pollution. The object of the study were soil samples taken in the area adjacent to the tracks on the section of the railway Bakhmach-Hrebinka near the station of the city Pyriatyn. Soil sampling was carried out in accordance with DSTU ISO 10381-1: 2004. Assessment of soil contamination was assessed by bioindication using watercress test. According to the results of the research, it was found that the germination energy, seed germination and growth rates of watercress plants decreased on soil samples taken closer to the railway tracks. Weak levels of contamination were observed on soils selected at a distance of 5 meters from the railway, germination energy and seed germination were - 58 and 77%, respectively, the remaining samples did not show a significant effect on the sowing quality of watercress seeds. It was found that in plants grown on sample 4 (100 meters from the railway tracks) the length of the root system was 46 mm, while in samples 1, 2, 3 the indicator decreased respectively by 50, 32 and 9%, . The aerial part of watercress plants grown on 4 samples was 42 mm, while in samples 1,2,3, the figure decreased respectively by 43, 9 and 2%, . Therefore, it can be argued that the soil samples taken in the area adjacent to the tracks on the railway line Bakhmach-Grebinka, at a distance of 5 meters from the tracks, are low-contaminated, while the rest of the samples according to research are unpolluted and meet sanitary requirements on planning and development of settlements.


2018 ◽  
Vol 22 (3) ◽  
pp. 122-124
Author(s):  
Jasmina Milenković ◽  
Rade Stanisavljević ◽  
Jordan Marković ◽  
Mirjana Petrović ◽  
Tanja Vasić ◽  
...  

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 394
Author(s):  
Mayton ◽  
Amirkhani ◽  
Loos ◽  
Crawford ◽  
Crawford ◽  
...  

The focus of this research was to evaluate genotypes for cold-tolerant germination from wild switchgrass (Panicum virgatum L.) populations collected in the Northeast USA. Switchgrass nurseries were established in 2008 and 2009 with seed collected from native stands of switchgrass in the Northeast USA between 1991 and 2008. Switchgrass seed harvested from individual genotypes was evaluated for cold-tolerant germination in a series of laboratory experiments. Germination assays of seed of 13 switchgrass genotypes harvested in the fall of 2016 are the primary focus of this reported research. The selected genotypes were evaluated for cold-tolerant seed germination in three experiments, during the spring of 2017, fall of 2017 and spring of 2018, (with and without stratification) using a 10/15 °C regime with a 12 h photoperiod. Germination tests showed that several genotypes had significantly higher percentage germination as well as faster germination rates expressed as T50 (number of days required to reach 50% maximum germination) when compared to Cave-in-Rock, a moderately sensitive cold-tolerant commercial cultivar established in the original switchgrass nursery as a control. A final germination test was conducted to compare seed from the original population (no selection cycle 0), with one of the top performing cold-tolerant germination genotypes, and a commercial cultivar, ‘Espresso’, developed for low seed dormancy and low temperature germination. In this test, the selected genotype had significantly higher percentage germination in the stratified treatment and was not significantly different than Espresso in the non-stratified test. These data indicate successful selection for cold-tolerant germination in switchgrass genotypes from native germplasm collected in the Northeast USA.


Author(s):  
А. О. Семенов ◽  
Г. М. Кожушко ◽  
Т. В. Сахно

У роботі досліджено вплив трьох різних областей С (200–280 нм), В (280–320 нм) та А (320м400 нм) ультрафіолетового опромінення насіння. Встановлено, що УФ-випромінювання, незалежно від спектрального діапазону, позитивно впливає на біологічні процеси: енергія проростання, здатність до проростання та схожість насіння для області С більша на 5–11%, у порівнянні з УФ областями А і В, за однакових доз УФ-опромінювання. One of the main tasks of the agricultural complex is to increase the quantity and quality of crop production. Great interest in stimulating growth and increasing the resistance of plants to external factors and increasing the productivity of agricultural crops is the use of optical radiation – pre-sowing processing of seeds of crops by ultraviolet radiation. The effect of three different regions C (200–280 nm), B (280–320 nm) and A (320–400 nm) of ultraviolet irradiation of seeds was investigated. It is established that UV irradiation irrespective of the spectral range positively affects biological processes: germination energy, germination capacity and seed germination. The energy of germination, the capacity to germinate and the germination of seeds were determined in laboratory conditions. These indices were compared for seeds irradiated in different energy regions A, B, C of ultraviolet radiation at the same radiation dose of 120 J/m2 with control samples (without irradiation). Investigations of germination energy, germination capacity and seed germination showed that UV irradiation in different energy regions A, B, C at doses of 120 J/m2 positively affects rape seeds, as the germination energy increases by 23–31 %, the capacity to sprouting by 14–25 %, and the similarity increases by 8–17 %. In addition, studies at similar doses of UV irradiation show that the germination energy for the C region is 5–8 % higher in comparison with the UV regions A and B, while the germination capacity for the C region is greater by 9–11 %, and the similarity for region C is large by 9.2 % compared with region B, and by 6.6 in comparison with region A. Comparing the effect of the energy regions of different UV ranges on pre-sowing seed irradiation, it can be stated that UV irradiation irrespective of the spectral range positively affects the germination energy, germination capacity and seed germination.


2020 ◽  
pp. 35-41
Author(s):  
V. Dryga

The aim of the research is determination of millet seed scarification efficiency depending on the modes – the amount of seeds surface removal. Scarification implies mechanical damage to the solid seed coat surface and its partial removal is applied to increase the intensity of seed germination. Studies oo implying this method on the millet seeds of the rod-shaped millet have shown that the seeds germination energy and sprouting significantly increase. On average for 15 experiments, these indicators increased by 7 % compared to the control – without scarification. A comprehensive study of the effect of scarification and vegetation years on germination energy and seed germination revealed that both the vegetation years and scarification influence these parameters. The share of factors influence on the seeds germination showed that the "vegetation year" factor was the dominant and made 70.9 %, the impact of scarification factor made only 6.9 %. The seeds quality in both control and scarification variants varied significantly depending on the vegetation years of the millet. The highest germination energy and sprouting were in the seeds of the seventh year, the lowest – the fourth year of the crop vegetation. To investigate the impact of scarification on the seeds quality, a series of one-factor experiments were conducted in which the seeds of one batch were used. It is found that scarification with removing 2.8–8.8 % of the seed coat, provided a significant increase in seed germination compared to the control (without scarification). Removal of 8.8 % of the seed coat surface provided 9 % increase in germination energy and 6 % in sprouting increase; removal of 2.8 % of the surface provided germination energy increased by 11 % and sprouting increase by 8 %. The impact of the scarification factor on the germination energy and sprouting was significant and amounted to 50.0 and 84.0 %, respectively. The application of scarification of the millet seed provides a reliable increase in its germination energy and sprouting, but this method does not solve the problem of reducing the seeds biological dormancy, so it is advisable to continue the study on the nature of the seed dormancy in order to clarify tme possibility of managing this phenomenon and improve the technology of obtaining sufficient amount of high germination seeds. Key words: sprouting, germination energy, scarification, seed coat, seed mass.


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