scholarly journals Modified Bean Seed Protein Phaseolin Did Not Accumulate Stably in Transgenic Tobacco Seeds after Methionine Enhancement Mutations

2015 ◽  
Vol 06 (05) ◽  
pp. 640-650 ◽  
Author(s):  
Eric Lasserre ◽  
T. S. Ko ◽  
John M. Dyer ◽  
Norimoto Murai
Keyword(s):  
Transgenic Tobacco ◽  
Seed Protein ◽  
Bean Seed ◽  
Tobacco Seeds

Targeting and processing of membrane-anchored YFP fusion proteins to protein storage vacuoles in transgenic tobacco seeds

2005 ◽  
Vol 15 (4) ◽  
pp. 361-364 ◽  
Author(s):  
Ka Leung Fung ◽  
Yin Fun Yim ◽  
Yu Chung Tse ◽  
Yansong Miao ◽  
Samuel S.M. Sun ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Fusion Proteins ◽  
Seed Protein ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Protein Storage ◽  
Protein Storage Vacuoles ◽  
Pharmaceutical Proteins ◽  
Tobacco Seeds ◽  
Foreign Proteins

Seeds that store proteins in protein storage vacuoles are attractive bioreactors for producing and storing large amounts of pharmaceutical proteins. However, foreign proteins expressed in transgenic plants are subjected to the delivery and modification processes present within plant cells. Here, it is demonstrated that unique membrane sequences deliver a yellow fluorescent protein (YFP) to the seed protein storage vacuoles in transgenic tobacco (Nicotiana tabacum L.) plants, where the YFP is then separated from its membrane anchors. This precise targeting and separation is required for the successful delivery of useful proteins to seed protein storage vacuoles for their stable accumulation in transgenic crops.

Increased seed cytokinin levels in transgenic tobacco influence embryo and seedling development

2002 ◽  
Vol 29 (9) ◽  
pp. 1107 ◽  
Author(s):  
Qing-Hu Ma ◽  
Zhan-Bing Lin ◽  
De-Zhi Fu
Keyword(s):  
Transgenic Tobacco ◽  
Dry Weight ◽  
Polymerase Chain Reaction Analysis ◽  
Single Copy ◽  
Ipt Gene ◽  
Specific Expression ◽  
Tobacco Seeds ◽  
Cell Layers ◽  
Polymerase Chain ◽  
Transgenic Embryos

Seed-specific expression of a heterologous vicilin-isopentenyl transferase (ipt) gene has been determined in transgenic tobacco lines 7 and 16. Genetic analysis of self-fertilized progeny showed that a single copy of the vicilin-ipt gene had been integrated, and T2 progeny had become homozygous for the transgene. Stable inheritance of the vicilin-ipt gene in T3 progeny was verified by polymerase chain reaction analysis. Seed cytokinin levels were 2-3-fold higher than control levels. Cytological analyses revealed a significant increase in the number of plerome cell layers and enlargement of diameter in transgenic embryos compared with controls. Dry weight of mature seeds and subsequent seedling growth was increased significantly. This correlated with the level of vicilin-ipt overexpression and increased cytokinin levels in transgenic tobacco seeds. The results suggest a crucial role for cytokinins in regulation of tobacco embryo development.

Analysis of kafirin promoter activity in transgenic tobacco seeds

1996 ◽  
Vol 32 (6) ◽  
pp. 1029-1035 ◽  
Author(s):  
Richard T. DeRose ◽  
Dilara Begum ◽  
Timothy C. Hall
Keyword(s):  
Transgenic Tobacco ◽  
Promoter Activity ◽  
Tobacco Seeds

2S storage protein gene of Douglas-fir: characterization and activity of promoter in transgenic tobacco seeds

2004 ◽  
Vol 42 (5) ◽  
pp. 417-423 ◽  
Author(s):  
Malinee Chatthai ◽  
Benjamin S. Forward ◽  
Dmytro Yevtushenko ◽  
Ivan Stefanov ◽  
Lubica Osuska ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Storage Protein ◽  
Protein Gene ◽  
Douglas Fir ◽  
Storage Protein Gene ◽  
Tobacco Seeds

Protective effect of oral administration of transgenic tobacco seeds against verocytotoxic Escherichia coli strain in piglets

2013 ◽  
Vol 38 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Luciana Rossi ◽  
Vittorio Dell’Orto ◽  
Simona Vagni ◽  
Vittorio Sala ◽  
Serena Reggi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Oral Administration ◽  
Protective Effect ◽  
Transgenic Tobacco ◽  
Coli Strain ◽  
Tobacco Seeds

scholarly journals CAP2 enhances germination of transgenic tobacco seeds at high temperature and promotes heat stress tolerance in yeast

FEBS Journal ◽  
2009 ◽  
Vol 276 (18) ◽  
pp. 5252-5262 ◽  
Author(s):  
Rakesh Kumar Shukla ◽  
Vineeta Tripathi ◽  
Deepti Jain ◽  
Rajiv Kumar Yadav ◽  
Debasis Chattopadhyay
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Tobacco Seeds ◽  
Heat Stress Tolerance

Developmentally regulated expression of a sunflower 11S seed protein gene in transgenic tobacco

1990 ◽  
Vol 222 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Molly A. Bogue ◽  
Raymond A. Vonder Haar ◽  
Michael L. Nuccio ◽  
Lawrence R. Griffing ◽  
Terry L. Thomas
Keyword(s):  
Transgenic Tobacco ◽  
Seed Protein ◽  
Protein Gene ◽  
Developmentally Regulated ◽  
Regulated Expression

Genetic control of bean seed protein

1988 ◽  
Vol 7 (2) ◽  
pp. 93-116 ◽  
Author(s):  
Thomas C. Osborn ◽  
John W.S. Brown
Keyword(s):  
Genetic Control ◽  
Seed Protein ◽  
Bean Seed
Sign in / Sign up

Export Citation Format

Share Document