Genetic diversity of natural populations of Machilus thunbergii, an endangered tree species in eastern China, determined with ISSR analysis

Abstract Maire yew (Taxus wallichiana var. mairei (Lemée H. Léveillé.) L. K. Fu et Nan Li) is a rare and endangered tree species, and it is also a precious timber species in China. We used 13 microsatellites to assess the genetic diversity and differentiation of 665 Maire yew samples from 18 natural populations. A total of 291 alleles were detected. The average number of alleles (Na=22.39), expected heterozygosity (He=0.74), polymorphic information content (PIC = 0.86) and Shannon diversity index (I = 1.66) of the loci indicated a high level of genetic diversity in natural Maire yew populations. Moreover, gene flow was more active among populations (Nm=1.62) than within populations. Among the 18 populations, the Xinfeng population in Jiangxi Province has the highest genetic diversity. Although each of the studied populations should be protected from further deforestation and agricultural expansion, the Xinfeng population deserves the highest conservation priority. The results based on analysis of molecular variance showed that genetic variation occurred mainly within populations (84.90%; P < 0.001), which indicated that the degree of genetic differentiation of the natural populations of Maire yew was low. Based on UPGMA, the 18 populations were categorized into 4 groups. A Mantel test showed that there was no significant correlation between standard genetic distance and geographical distance or altitude differences among the populations. The genetic clustering results also indicated that the genetic relationship followed a north to south clustered trend. The information presented here forms the basis for the development of genetic guidelines for appropriate conservation programs.

Download Full-text

Genetic diversity and differentiation of an endangered tree species, Afzelia xylocarpa (Kurz) craib in Thailand revealed by nuclear microsatellite markers

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb12.1919 ◽

2014 ◽

Vol 13 (3) ◽

pp. 366-377 ◽

Cited By ~ 6

Author(s):

Pakkad Greuk ◽

Kanetani Seiichi ◽

Elliott Stephen

Keyword(s):

Genetic Diversity ◽

Microsatellite Markers ◽

Tree Species ◽

Endangered Tree ◽

Endangered Tree Species ◽

Nuclear Microsatellite

Download Full-text

Discovery of novel SSR markers from transcriptome data of Astronium fraxinifolium Schott, a threatened tree species in Brazil

10.21203/rs.3.rs-65083/v1 ◽

2020 ◽

Author(s):

Maiara Cornacini ◽

Ricardo Oliveira Manoel ◽

Marcelo Alcantara ◽

Mário Moraes ◽

Edvaldo Silva ◽

...

Keyword(s):

Ssr Markers ◽

Tree Species ◽

Natural Populations ◽

Transcriptome Data ◽

Rna Seq ◽

Endangered Tree ◽

Genetic Studies ◽

Endangered Tree Species ◽

Ssr Loci ◽

First Time

Abstract Background Astronium fraxinifolium is an endangered tree species from Brazil. Due to its high importance for environmental reforestation, as well as for the use of its wood, it is necessary to implement management programs for conservation of this species. Simple sequence repeats (SSR) or microsatellite markers have been widely used in population genetic studies across diverse organisms. In this study, we reported for the first time SSR markers for A. fraxinifolium as well as its frequency and distribution from transcriptome data. Results More than 125 thousand RNA-seq sequences derived microsatellites, with predominant distribution of trinucleotides repeats. From the initial screening, we selected 20 microsatellite loci, validated and evaluated genetic indexes in two natural populations. All loci were polymorphic, ranging from four to eleven alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively. Genetic differentiation between populations (= 0.363) showed higher diversity within than among populations. Conclusions The developed SSR loci from RNA-seq consists in a base for future studies of genetic diversity and population structure, mating system and gene flow in A. fraxinifolium populations as well as related species, aiming the conservation and management of the species.

Download Full-text

Genetic diversity and demographic analysis of an endangered tree species Diplopanax stachyanthus in subtropical China: implications for conservation and management

Conservation Genetics ◽

10.1007/s10592-018-1133-0 ◽

2018 ◽

Vol 20 (2) ◽

pp. 315-327 ◽

Cited By ~ 1

Author(s):

Li Feng ◽

Zhi-Yao Xu ◽

Li Wang

Keyword(s):

Genetic Diversity ◽

Tree Species ◽

Demographic Analysis ◽

Subtropical China ◽

Endangered Tree ◽

Endangered Tree Species ◽

Conservation And Management

Download Full-text

Genetic diversity of Dalbergia monticola (Fabaceae) an endangered tree species in the fragmented oriental forest of Madagascar

Forest Diversity and Management ◽

10.1007/978-1-4020-5208-8_4 ◽

2007 ◽

pp. 49-68 ◽

Cited By ~ 1

Author(s):

Olivarimbola Andrianoelina ◽

Hery Rakotondraoelina ◽

Lolona Ramamonjisoa ◽

Jean Maley ◽

Pascal Danthu ◽

...

Keyword(s):

Genetic Diversity ◽

Tree Species ◽

Endangered Tree ◽

Endangered Tree Species

Download Full-text

Study on the Genetic Structure Based on Geographic Populations of the Endangered Tree Species: Liriodendron chinense

Forests ◽

10.3390/f12070917 ◽

2021 ◽

Vol 12 (7) ◽

pp. 917

Author(s):

Peng-Yan Zhou ◽

Li-Xing Hui ◽

Shu-Jing Huang ◽

Zhou-Xian Ni ◽

Fa-Xin Yu ◽

...

Keyword(s):

Genetic Diversity ◽

Genetic Structure ◽

Natural Populations ◽

Systematic Research ◽

Endangered Tree ◽

Endangered Tree Species ◽

Expected Heterozygosity ◽

Liriodendron Chinense ◽

Geographic Populations ◽

Degree Of Differentiation

Liriodendron chinense (Hemsley) Sargent is a Class II protected plant in China as natural populations are on the verge of extinction. There is still a lack of systematic research on the genetic resources of its geographic populations. In this study, we used 20 pairs of SSR markers with high polymorphism to analyze a total of 808 L. chinense samples from 22 regions, and 63 Liriodendron tulipifera Linn samples from 2 regions were used as a comparison group. The results revealed a total of 78 alleles in L. chinense, and the average expected heterozygosity (He) was 0.558, showing a low level of genetic diversity. The degree of differentiation of L. chinense was high, with the differentiation coefficient (Fst) as high as 0.302, which is related to the low gene flow (Nm = 0.578). Based on the genetic structure, principal coordinate analysis (PCoA) and phylogenetic analysis of 24 Liriodendron spp. populations, L. chinense and L. tulipifera had obvious differentiation, while the differentiation between L. chinense geographic populations was very large and irregular. Inbreeding appears within the geographic populations, and the level of genetic diversity is very low. In order to protect the genetic diversity of L. chinense, in addition to protecting the existing population as much as possible, artificial cultivation should introduce materials from multiple populations.

Download Full-text