scholarly journals Discovery and characterization of new microsatellite loci in Dipteryx alata Vogel (Fabaceae) using next-generation sequencing data

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
R.A. Guimarães ◽  
M.P.C. Telles ◽  
A.M. Antunes ◽  
K.M. Corrêa ◽  
C.V.G. Ribeiro ◽  
...  
PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2019 ◽  
Author(s):  
Christine Ewers-Saucedo ◽  
John D. Zardus ◽  
John P. Wares

Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacleChelonibia testudinaria(Linnaeus, 1758).Chelonibia testudinariaappears to be a host generalist, and has an unusual sexual system, androdioecy. Genetic studies on host specificity and mating behavior are impeded by the lack of fine-scale, highly variable markers, such as microsatellite markers. In the present study, we discovered thousands of new microsatellite loci from next-generation sequencing data, and characterized 12 loci thoroughly. We conclude that 11 of these loci will be useful markers in future ecological and evolutionary studies onC. testudinaria.


PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0185211 ◽  
Author(s):  
Camille Tumiotto ◽  
Lionel Riviere ◽  
Pantxika Bellecave ◽  
Patricia Recordon-Pinson ◽  
Alice Vilain-Parce ◽  
...  

2016 ◽  
Author(s):  
Christine Ewers-Saucedo ◽  
John D Zardus ◽  
John P Wares

Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758). Chelonibia testudinaria appears to be a host generalist, and has a unusual sexual system, androdioecy. Genetic studies on host specificity and mating behavior are impeded by the lack of fine-scale, highly variable markers. In the present study, we discovered thousands of new microsatellite loci from next-generation sequencing data, and characterized 12 loci thoroughly. We conclude that 11 of these loci will be useful markers in future ecological and evolutionary studies on C. testudinaria.


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