Bidirectional lncRNA Transfer between Cuscuta Parasites and Their Host Plant

Dodder species (Cuscuta spp.) are holoparasites that have extensive material exchange with their host plants through vascular connections. Recent studies on cross-species transfer have provided breakthrough insights, but little is known about the interaction mechanisms of the inter-plant mobile substances in parasitic systems. We sequenced the transcriptomes of dodder growing on soybean hosts to characterize the long non-coding RNA (lncRNA) transfer between the two species, and found that lncRNAs can move in high numbers (365 dodder lncRNAs and 14 soybean lncRNAs) in a bidirectional manner. Reverse transcription-polymerase chain reaction further confirmed that individual lncRNAs were trafficked in the dodder–soybean parasitic system. To reveal the potential functions of mobile transcripts, the Gene Ontology terms of mobile lncRNA target genes were predicted, and mobile dodder target genes were found to be mainly enriched in “metabolic process”, “catalytic activity”, “signaling”, and “response to stimulus” categories, whereas mobile soybean target genes were enriched in organelle-related categories, indicating that specific mobile lncRNAs may be important in regulating dodder parasitism. Our findings reveal that lncRNAs are transferred between dodder and its host soybean plants, which may act as critical regulators to coordinate the host–dodder interaction at the whole parasitic level.

Effect of interface-active proteins on the salt crystal size in waterborne hybrid materials

Aqueous processes yielding hybrid or composite materials are widespread in natural environments and their control is fundamental for a multiplicity of living organisms. Their design and in vitro engineering require knowledge about the spatiotemporal evolution of the interactions between the involved liquid and solid phases and, especially, the interphases governing the development of adhesion during solidification. The present study illustrates the effects of distinct proteins on the precipitation of sodium chloride encompassing the size, shape and distribution of halite crystals formed during the drying of droplets containing equally concentrated saline protein solutions. The precipitates obtained from aqueous sodium chloride formulations buffered with tris(hydroxymethyl)aminomethane (Tris) contained either bovine serum albumin (BSA), fibrinogen or collagen and were characterized with respect to their structure and composition using optical and electron microscopy as well as x-ray analysis. The acquired findings highlight that depending on the protein type present during droplet drying the halite deposits predominantly exhibit cubic or polycrystalline dendritic structures. Based on the phenomenological findings, it is suggested that the formation of the interphase between the growing salt phase and the highly viscous saline aqueous jelly phase containing protein governs not only the material transport in the liquid but also the material exchange between the solid and liquid phases.

Denitrification of Permeable Sand Sediment in a Headwater River Is Mainly Influenced by Water Chemistry, Rather Than Sediment Particle Size and Heterogeneity

Sediment particle size and heterogeneity play an important role in sediment denitrification through direct and indirect effects on, for example, the material exchange rate, environmental gradients, microbial biomass, and grazing pressure. However, these effects have mostly been observed in impermeable sediments. On the other hand, the material exchange of permeable sediments is dominated by advection instead of diffusion, with the exchange or transport rates exceeding those of diffusion by two orders of magnitude relative to impermeable sediments. The impact of permeable sediment particle size and heterogeneity on denitrification remains poorly understood, especially at the millimeter scale. Here, we conducted an in situ control experiment in which we sorted sand sediment into four homogeneous-particle-sizes treatments and four heterogeneous treatments. Each treatment was deployed, in replicate, within the riffle in three different river reaches with contrasting physicochemical characteristics. After incubating for three months, sediment denitrifier communities (nirS, nirK, nosZ), denitrification gene abundances (nirS, nirK, nosZ), and denitrification rates in all treatments were measured. We found that most of the denitrifying microbes in permeable sediments were unclassified denitrifying microbes, and particle size and heterogeneity were not significantly correlated with the functional gene abundances or denitrification rates. Water chemistry was the key controlling factor for the denitrification of permeable sediments. Water NO3−-N directly regulated the denitrification rate of permeable sediments, instead of indirectly regulating the denitrification rate of sediments by affecting the chemical characteristics of the sediments. Our study fills a knowledge gap of denitrification in permeable sediment in a headwater river and highlights that particle size and heterogeneity are less important for permeable sediment denitrification.

Quantitative theory for the diffusive dynamics of liquid condensates

Key processes of biological condensates are diffusion and material exchange with their environment. Experimentally, diffusive dynamics are typically probed via fluorescent labels. However, to date, a physics-based, quantitative framework for the dynamics of labeled condensate components is lacking. Here we derive the corresponding dynamic equations, building on the physics of phase separation, and quantitatively validate the related framework via experiments. We show that by using our framework we can precisely determine diffusion coefficients inside liquid condensates via a spatio-temporal analysis of fluorescence recovery after photobleaching (FRAP) experiments. We showcase the accuracy and precision of our approach by considering space- and time-resolved data of protein condensates and two different polyelectrolyte-coacervate systems. Interestingly, our theory can also be used to determine a relationship between the diffusion coefficient in the dilute phase and the partition coefficient, without relying on fluorescence measurements in the dilute phase. This enables us to investigate the effect of salt addition on partitioning and bypasses recently described quenching artifacts in the dense phase. Our approach opens new avenues for theoretically describing molecule dynamics in condensates, measuring concentrations based on the dynamics of fluorescence intensities, and quantifying rates of biochemical reactions in liquid condensates.

Accumulator, Transporter, Substrate, and Reactor: Multidimensional Perspectives and Approaches to the Study of Bark

Woody ecosystems have a relatively thin but aerially extensive and dynamic layer of bark that, like leaves, regulates material exchange at the interface of air, water, and biota. Through interception, retention, and leaching of materials and interactions with epiphytic communities, bark alters the chemistry and composition of water draining over its surface during precipitation. This mini-review explores different perspectives and approaches to the study of bark and what they reveal about the myriad ways bark surfaces influence the quality of sub-canopy precipitation. Observational studies conducted over the past five decades in the fields of environmental science, ecohydrology, epiphyte ecology, and microbiology demonstrate that bark is an accumulator, transporter, substrate, and reactor. Bark passively accumulates materials from the atmosphere, water, and canopies, and also serves as an active transport surface, exchanging materials laterally and longitudinally. In addition, bark substrates influence epiphyte diversity, composition, and distribution, which, in turn, affect material cycling. Bark surfaces are dynamic over time, changing in response to disturbances (e.g., insect outbreaks, aging, and tree death)—how such changes influence the chemical and elemental composition of throughfall and stemflow merits further study. Moving forward, integration of diverse perspectives and approaches is needed to elucidate the influence of bark surfaces on solute and particulate transport and cycling within woody ecosystems.

Mongolian-Chinese Exchanges in History Studies during the Socialist Period: Mutual Understanding and Divergence (the 1950s)

This paper studies the attempts of the MPR and the PRC to promote historical exchanges in the 1950s based on their shared ideology of proletarian internationalism and historical materialism. Specifically, this research analyzes the initial historical exchanges between the two countries in the form of material exchange in the early 1950s, and further discusses about the cooperation between Soviet, Mongolian and Chinese scholars to compile a three-volume Mongolian history in the second half of the 1950s, which pushed the Mongolian-Chinese historical exchanges to a new level. Meanwhile, the research also takes into account the influence of Sino-Soviet relations and historical differences between Mongolia and China on their historical exchanges, especially the cooperation in compiling three-volume Mongolian history. Archival documents in Mongolian and Chinese languages preserved in Mongolia are used as the main research materials for this paper. Социализмын үеийн Монгол, Хятадын түүх судлалын харилцаа: Ойлголцол ба зөрчил (1950-иад он) Хураангуй: Энэхүү өгүүлэлд БНМАУ, БНХАУ-ын хооронд дипломат харилцаа тогтоосны дараах 10 орчим жилийн хугацаанд хоёр улс социалист орнуудын хувиар түүхэн материализмын онол хийгээд пролетарийн интернационализмын зарчмыг үзэл баримтлал болгосон ойлголцлын үндсэн дээр хамтран түүх судлалыг хөгжүүлэх гэсэн оролдлогын тухайд тусгайлан өгүүлэх болно. Чингэхдээ, 1950-иад оны эхэн хагаст материал солилцох байдлаар түүх судлалын харилцааг эхлүүлснийг тодруулан, улмаар 1950-иад оны хоёрдугаар хагаст “БНМАУ-ын түүхийн гурван боть”-ийг Монгол, Хятад, Зөвлөлтийн эрдэмтэд хамтран бичих төлөвлөгөөний хүрээнд Монгол-Хятадын түүх судлалын харилцаа эрчимжсэн ч тухайн цагийн олон улсын харилцааны нөхцөл байдал хийгээд хоёр талын түүхэн ойлголтын талаарх зарчмын зөрөө нь энэхүү харилцааг хөгжүүлэх явцад хэрхэн сорилт болж байсан, тэрхүү сорилтыг даван туулах гэсэн эрмэлзлэлийн тухайд архивын баримтуудад голлон тулгуурлаж, задлан шинжлэл хийсэн болно. Түлхүүр үгс: БНМАУ, БНХАУ, түүх судлалын солилцоо, БНМАУ-ын түүхийн гурван боть, түүхэн материализм, социалист лагер

Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood

As the medium of material exchange between mother and fetus, umbilical cord blood is closely connected with fetal development. microRNA (miRNA) has a wide range of biological functions and has high flow characteristics. Small RNA sequencing of pig umbilical venous blood (UVB) and umbilical arterial blood (UAB) revealed that a total of 302 miRNAs were identified, and 106 and 22 miRNAs were specifically expressed in the UVB and UAB, respectively. Using the two methods of differential expression multiple and differential expression percentage, it is found that only 35% of the highly expressed miRNAs in the UVB by the two analysis modes overlap, but 56.25% of the enriched signal pathways are the same. Only 20% of the highly expressed miRNAs in the UAB overlap, but 62.07% of the signal pathways are the same. Further analysis revealed that miR-423 can be used as a characteristic miRNA of UVB and has the potential to treat muscle-related diseases. miR-122-5p can be used as a characteristic miRNA of UAB and may help to improve liver- and brain-related diseases. In summary, these results enrich understanding of miRNA in mother–fetal communication and provide a reference for the development and application of porcine cord blood products.

Franco-British responses to chemical warfare 1915–8, with special reference to the medical services, casualty statistics and the threat to civilians

This dual-focussed examination will critically compare and contrast the British Royal Army Medical Corps and the French Medical Service’s involvement in the Western Front chemical campaign between 1915 and 1918. Because the Anglophone historiography has tended to marginalise the French contribution to the allied chemical war, this article will attempt to re-balance the historical narrative by emphasising the collective nature and importance of this joint Franco-British enterprise. By interrogating a raft of under-utilised primary evidence in the French and British archives, this investigation will contribute to the ‘alliance literature’ by arguing that when it comes to aspects of the Franco-British chemical war such as the co-operation of the medical services, the appellation ‘together but alone’ does not fully hold. The article will explore avenues of the two national armies evolving process of mutual medical assistance, material exchange and scientific collaboration. The striking similarity of French and British gas casualty statistics is highlighted with reference to the overall congruence of their anti-gas strategies – notwithstanding the problematic nature of these statistics. In addition to enhancing soldiers’ resistance to the poison gas threat on the battlefield, the Medical Services were also responsible for the protection of local civilians in the war zones. With reference mainly to France, this investigation will discuss the significance and implications of the poison gas threat to the home front. Finally, the impact of chemical weapon production upon civilian war workers in France and Britain will be commented upon.

Treasuring Yemen: Notes on Exchange and Collection in Rasūlid Material Culture

Often distinguished by their characteristic five-petalled rosette emblems, objects dedicated to the Rasūlid sultans of Yemen in Egypt or Syria have long been identified as a distinct corpus in histories of Islamic art. Whether treated singly or as a group, these objects have usually been positioned in the periphery of discussions about Mamlūk luxury arts or cited briefly as evidence of diplomatic relations between the Mamlūk and Rasūlid leadership. Perhaps reflecting a general marginalization of South Arabia in the historiographic traditions of Islamic art scholarship, narratives centered on the imperial Mamlūk enterprise tend to overshadow both the Rasūlid context for these objects and the complexities of their global material histories. This essay explores these two themes together, drawing broadly on visual culture connected with the Rasūlid court to analyze a selection of case studies. It reviews art historical literature on the Rasūlid-Mamlūk corpus as reflected in European and North American scholarship, outlines the various modalities by which this material exchange is understood to have taken place, explores the setting for Rasūlid patronage and collection of such objects, and examines their material afterlives.