Research Article Association of MC4R, FABP3 and DGAT1 gene polymorphisms with reproductive traits in two domestic pig lines

The aim of the study was to investigateGDF9gene polymorphisms and their association with reproductive traits in chicken using DNA sequencing. A total of 279 Dongxiang blue-shelled (DX) chickens and 232 Luhua (LH) chickens were used for validation. We detected 15 single nucleotide polymorphisms (SNPs): nine SNPs were previously unreported in chicken, two were missense mutations, and only three exhibited significant associations with reproductive traits. G.17156387C>T was significantly associated with age at first egg (AFE) and weight of first egg (WFE) in both breeds. Birds carrying the CC genotype exhibited higher AFE and WFE values than those with the TT genotype. The SNP g.17156427A>G exhibited an association with egg weight at 300 days of age (EWTA) in DX but not in LH chickens. The SNP g.17156703A>C affected the AFE and EN (total number of eggs at 300 days of age) in DX chickens. In addition, certain diplotypes significantly affected AFE, BWTA (body weight at 300 days of age), and EN in both breeds. RT-PCR results showed that theGDF9gene was highly expressed in stroma with cortical follicles (STR) and prehierarchal follicles. These results provided further evidence that theGDF9gene is involved in determining reproductive traits in chicken.

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Corrigendum to “Follicle-stimulating hormone (FSHβ) gene polymorphisms and associations with reproductive traits in Rex rabbit” [Anim. Reprod. Sci. 207 (2019) 36–43]

Animal Reproduction Science ◽

10.1016/j.anireprosci.2019.106150 ◽

2019 ◽

Vol 209 ◽

pp. 106150

Author(s):

Xiaoyan Niu ◽

Graeme B. Martin ◽

Wenzhong Liu ◽

Mark A. Henryon ◽

Keliang Ren

Keyword(s):

Gene Polymorphisms ◽

Follicle Stimulating Hormone ◽

Reproductive Traits ◽

Rex Rabbit ◽

Stimulating Hormone

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Effect of candidate gene polymorphisms on reproductive traits in a Large White pig population

Animal Science Journal ◽

10.1111/asj.12580 ◽

2016 ◽

Vol 87 (12) ◽

pp. 1455-1463 ◽

Cited By ~ 2

Author(s):

Shuji Sato ◽

Takashi Kikuchi ◽

Yoshinobu Uemoto ◽

Satoshi Mikawa ◽

Keiichi Suzuki

Keyword(s):

Candidate Gene ◽

Gene Polymorphisms ◽

Reproductive Traits ◽

Large White ◽

Large White Pig

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Estrus Behaviour and Reproductive Traits of Pulawska Gilts Associated with Selected Gene Polymorphisms

Annals of Animal Science ◽

10.2478/aoas-2020-0066 ◽

2021 ◽

Vol 21 (1) ◽

pp. 159-172

Author(s):

Marek Babicz ◽

Marcin Pastwa ◽

Magdalena Szyndler-Nędza ◽

Anna Kozubska-Sobocińska ◽

Barbara Danielak-Czech ◽

...

Keyword(s):

Genetic Structure ◽

Gene Polymorphisms ◽

Rflp Analysis ◽

Reproductive Traits ◽

The Other ◽

Molecular Analyses ◽

Native Breed ◽

Hair Roots ◽

Pcr Rflp ◽

Pcr Conditions

Abstract Searching for the associations between the gene polymorphism and the reproductive traits is essential in defining the genetic native breed specificity, which distinguishes them from the other breeds. The aim of our study was to determine the associations between mutations in the PRL, PRLR, PTGS2, FUT1 genes and sexual and periparturient activity in native Pulawska gilts. The analysis included 72 animals which gave birth to the first litter. Evaluation of the productive value of gilts accounted for indicators of sexual and periparturient activity as well as reproductive traits. The biological material for molecular analyses was obtained from the hair roots of the gilts. The genotype was verified by PCR RFLP analysis. The primers and PCR conditions were determined on the basis of available literature data. Statistically significant differences (P≤0.05) were found at the PRL locus: gilts of AA genotypes (Ins/Ins) at the PRL locus were characterised by longest farrowing duration compared to gilts of AB genotype (P≤0.05). The analysis of PRLR gene showed that gilts of TT genotype revealed a tendency for later occurrence of estrus signs (first and second estrus) and for the markedly longest farrowings (P≤0.05). With regard to PTGS2 and FUT1 loci, no significant differences were found in the parameters of sexual and periparturient activity of the gilts. However, gilts of FUT1 GG genotype gave birth to and reared the largest first litters (P≤0.05). The results of the studies expand the knowledge about the genetic structure and productivity of Pulawska gilts.

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Effects of follicle-stimulating hormone beta subunit and nuclear receptor coactivator 1 gene polymorphisms and expressions on pink-eyed white mink reproductive traits

Canadian Journal of Animal Science ◽

10.1139/cjas-2019-0088 ◽

2020 ◽

Vol 100 (4) ◽

pp. 683-690

Author(s):

Zongyue Liu ◽

Linling Liu ◽

Xingchao Song ◽

Zhishuang Huo ◽

Bo Cong ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Gene Polymorphisms ◽

Follicle Stimulating Hormone ◽

Reproductive Traits ◽

Beta Subunit ◽

Mrna Levels ◽

Chain Reaction ◽

Nuclear Receptor Coactivator ◽

Middle Term ◽

Polymerase Chain

The present study was designed to investigate comparative expressions of follicle-stimulating hormone beta subunit (FSHβ) and nuclear receptor coactivator 1 (NCOA1) genes by real-time polymerase chain reaction, using polymerase chain reaction single-strand conformation polymorphism methods to investigate the effects of gene polymorphisms on reproductive traits, including total number of kits born (TNB) and number of born alive (NBA) in pink-eyed white mink. Four single-nucleotide polymorphisms were identified in the FSHβ and NCOA1 genes. The g.1228G>A polymorphism of FSHβ was associated with NBA and TNB (P < 0.01). The g.151536T>C polymorphism of NCOA1 was associated with NBA and TNB (P < 0.01). The NCOA1 mRNA levels in hypothalamus, ovary, and uterus during the first half of gestation were higher than during the middle term and last half of gestation (P < 0.01). The FSHβ mRNA levels in the hypothalamus and uterus were higher during the first half of gestation than during the middle term and last half of gestation (P < 0.05). In conclusion, the g.1866T>C polymorphism of FSHβ and the g.151536T>C polymorphism of NCOA1 could be molecular markers for reproductive traits, and expressions of FSHβ and NCOA1 might be involved in the regulation of embryo attachment mechanisms in pink-eyed white mink breeding.

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Association between IGF-IR gene polymorphisms and productive and reproductive traits in Holstein cows

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352005000600011 ◽

2005 ◽

Vol 57 (6) ◽

pp. 772-777 ◽

Cited By ~ 1

Author(s):

W. Schoenau ◽

P.M. Porciuncula ◽

G. Zamberlan ◽

F.S. Mesquita ◽

V. Vieira ◽

...

Keyword(s):

Growth Factor ◽

Gene Polymorphisms ◽

Reproductive Traits ◽

Single Strand Conformation Polymorphism ◽

Calving Interval ◽

Lactation Period ◽

Chain Reaction ◽

Factor I ◽

Lactation Length ◽

Conformation Polymorphism

The association between single-strand conformation polymorphism (SSCP) in the gene of insulin-like growth factor-I receptor (IGF-IR) and age at first calving (AFC), calving interval (CI), lactation length (LL), and milk yield (MY) was studied using 106 graded Holstein females. The polimerase chain reaction (PCR) with specific initiating oligonucleotides, resulted an amplified fragment of 335pb. The population genotypes frequencies were 82.1% and 17.9%, for AA and AB genotypes, respectively. The frequency of A allele was 0.91 and 0.09 of B allele. No association between the identified polymorphism and AFC, CI, and MY was observed. The LL was positively associated (P<0.05) with the absence of B allele. Animals carrying the AA genotype presented a longer lactation period.

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